Restriction map for
pF1KB6886
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbrPI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BsaAI BsaI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BsrI BssHII
Bst1107I BstBI BstUI BstXI BstZ17I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeIII HgaI HincII HindII HindIII
HpaI KpnI KspI MaeI MaeII
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TfiI TspRI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AcsI 102 AAATTT [GA]^AATT[CT]
AcyI 21 GGCGCC G[GA]^CG[CT]C
ApoI 102 AAATTT [GA]^AATT[CT]
AspHI 61 GAGCTC G[AT]GC[AT]^C
BanI 21 GGCGCC G^G[CT][GA]CC
BbeI 21 GGCGCC GGCGC^C
BbsI 145 GAAGAC GAAGAC(2/6)
BbvCI 284 CCTCAGC CCTCAGC(-5/-2)
BglII 116 AGATCT A^GATCT
BlpI 253 GCTGAGC GC^T[ACGT]AGC
BpmI 374 CTCCAG CTGGAG(16/14)
Bpu1102I 253 GCTGAGC GC^T[ACGT]AGC
BpuAI 145 GAAGAC GAAGAC(2/6)
BsaBI 184 GATCGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 21 GGCGCC G[GA]^CG[CT]C
BsaJI 275 CCTCGG C^C[ACGT][ACGT]GG
BsaWI 236 ACCGGA [AT]^CCGG[AT]
BsgI 439 GTGCAG GTGCAG(16/14)
BsiHKAI 61 GAGCTC G[AT]GC[AT]^C
BsmFI 355 GGGAC GGGAC(10/14)
BsrBI 256 GAGCGG CCGCTC(-3/-3)
BsrFI 524 ACCGGC [GA]^CCGG[CT]
BstAPI 295 GCAGTAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 279 GGTTACC G^GT[ACGT]ACC
BstF5I 429 GGATG GGATG(2/0)
BstPI 279 GGTTACC G^GT[ACGT]ACC
CelII 253 GCTGAGC GC^T[ACGT]AGC
CfoI 22 GCGC GCG^C
Cfr10I 524 ACCGGC [GA]^CCGG[CT]
DraII 413 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 262 CTCTTC CTCTTC(1/4)
EcoO109I 413 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 279 GGTTACC G^GT[ACGT]ACC
FauI 48 GCGGG CCCGC(4/6)
FokI 429 GGATG GGATG(9/13)
HaeII 21 GGCGCC [GA]GCGC^[CT]
HhaI 22 GCGC GCG^C
Hin1I 21 GGCGCC G[GA]^CG[CT]C
HinP1I 22 GCGC G^CGC
HinfI 486 GAGTC G^A[ACGT]TC
HphI 489 TCACC GGTGA(8/7)
KasI 21 GGCGCC G^GCGCC
Ksp632I 262 CTCTTC CTCTTC(1/4)
MamI 184 GATCGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 150 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 290 CAGCTG C[AC]G^C[GT]G
NarI 21 GGCGCC GG^CGCC
PflMI 346 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 486 GAGTC GAGTC(4/5)
PpuMI 413 AGGACCC [GA]G^G[AT]CC[CT]
PstI 293 CTGCAG CTGCA^G
PvuII 290 CAGCTG CAG^CTG
SacI 61 GAGCTC GAGCT^C
SapI 261 GCTCTTC GCTCTTC(1/4)
SfaNI 6 GATGC GCATC(5/9)
SfcI 293 CTGCAG C^T[GA][CT]AG
SfoI 21 GGCGCC GGC^GCC
SspI 166 AATATT AAT^ATT
TaqI 228 TCGA T^CGA
Tsp509I 103 AATT ^AATT
TthHB8I 228 TCGA T^CGA
Van91I 346 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 79 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
287 CAGCAGCTG
AocI 418 CCTGAGG CC^T[ACGT]AGG
479 CCTCAGG
AvaII 321 GGACC G^G[AT]CC
414 GGACC
BanII 61 GAGCTC G[GA]GC[CT]^C
400 GAGCCC
BcnI 36 CCCGG CC^[GC]GG
44 CCCGG
BmyI 61 GAGCTC G[AGT]GC[ACT]^C
400 GAGCCC
BsiYI 346 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
384 CCACAATCAGG
BslI 346 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
384 CCACAATCAGG
Bsp1286I 61 GAGCTC G[AGT]GC[ACT]^C
400 GAGCCC
BssSI 560 CACGAG CACGAG(-5/-1)
64 CTCGTG
Bsu36I 418 CCTGAGG CC^T[ACGT]AGG
479 CCTCAGG
Cac8I 54 GCTGGC GC[ACGT]^[ACGT]GC
84 GCTGGC
Cfr13I 321 GGACC G^G[ACGT]CC
414 GGACC
Eco81I 418 CCTGAGG CC^T[ACGT]AGG
479 CCTCAGG
MseI 245 TTAA T^TAA
547 TTAA
NciI 36 CCCGG CC^[GC]GG
44 CCCGG
Sau96I 321 GGACC G^G[ACGT]CC
414 GGACC
Tru9I 245 TTAA T^TAA
547 TTAA
Tsp45I 365 GTGAC ^GT[GC]AC
488 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI AlwI BbvI BssKI
BstNI BstYI DdeI DpnII EcoRII
Fnu4HI HapII HpaII ItaI MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NdeII NlaIV Sau3AI
ScrFI TseI XhoII