Restriction map for
pF1KB7171
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoRV
FauI FseI FspI HindIII KasI
KpnI Ksp632I KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 3 GGCGTC G[GA]^CG[CT]C
Aor51HI 895 AGCGCT AGC^GCT
AspHI 1381 GAGCTC G[AT]GC[AT]^C
AvaII 201 GGACC G^G[AT]CC
AvrII 727 CCTAGG C^CTAGG
BclI 657 TGATCA T^GATCA
BcnI 480 CCGGG CC^[GC]GG
BlnI 727 CCTAGG C^CTAGG
BsaBI 973 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsiHKAI 1381 GAGCTC G[AT]GC[AT]^C
BspHI 1052 TCATGA T^CATGA
BspLU11I 314 ACATGT A^CATGT
BssSI 997 CTCGTG CACGAG(-5/-1)
CfoI 896 GCGC GCG^C
EaeI 1133 TGGCCG [CT]^GGCC[GA]
Eco47III 895 AGCGCT AGC^GCT
EcoNI 266 CCTACGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1355 GAATTC G^AATTC
EcoT22I 1257 ATGCAT ATGCA^T
FbaI 657 TGATCA T^GATCA
HaeII 895 AGCGCT [GA]GCGC^[CT]
HapII 480 CCGG C^CGG
HhaI 896 GCGC GCG^C
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HinP1I 896 GCGC G^CGC
HincII 744 GTTAAC GT[CT]^[GA]AC
HindII 744 GTTAAC GT[CT]^[GA]AC
HpaI 744 GTTAAC GTT^AAC
HpaII 480 CCGG C^CGG
MaeII 1389 ACGT A^CGT
MaeIII 1064 GTTAC ^GT[ACGT]AC
MamI 973 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 157 CAGCGG C[AC]G^C[GT]G
MspI 480 CCGG C^CGG
NciI 480 CCGGG CC^[GC]GG
NsiI 1257 ATGCAT ATGCA^T
NspI 314 ACATGT [GA]CATG^[CT]
PflMI 115 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 713 CTGCAG CTGCA^G
RcaI 1052 TCATGA T^CATGA
SacI 1381 GAGCTC GAGCT^C
TaqI 1128 TCGA T^CGA
TthHB8I 1128 TCGA T^CGA
Van91I 115 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 504 CCATAATAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 523 AAATTT [GA]^AATT[CT]
1355 GAATTC
AfaI 477 GTAC GT^AC
1436 GTAC
AflIII 314 ACATGT A^C[GA][CT]GT
1387 ACACGT
ApoI 523 AAATTT [GA]^AATT[CT]
1355 GAATTC
BanII 58 GAGCCC G[GA]GC[CT]^C
1381 GAGCTC
BmyI 58 GAGCCC G[AGT]GC[ACT]^C
1381 GAGCTC
BseRI 205 CTCCTC GAGGAG(10/8)
1378 GAGGAG
BsmI 1085 GAATGC GAATGC(1/-1)
461 GCATTC
Bsp1286I 58 GAGCCC G[AGT]GC[ACT]^C
1381 GAGCTC
BspMI 780 ACCTGC ACCTGC(4/8)
272 GCAGGT
BstYI 567 AGATCC [GA]^GATC[CT]
633 AGATCC
DraI 775 TTTAAA TTT^AAA
879 TTTAAA
HgaI 4 GCGTC GACGC(5/10)
1270 GCGTC
HphI 191 TCACC GGTGA(8/7)
796 TCACC
MflI 567 AGATCC [GA]^GATC[CT]
633 AGATCC
NcoI 49 CCATGG C^CATGG
77 CCATGG
RsaI 477 GTAC GT^AC
1436 GTAC
SfaNI 153 GATGC GCATC(5/9)
859 GATGC
SfcI 494 CTACAG C^T[GA][CT]AG
713 CTGCAG
TfiI 393 GAATC G^A[AT]TC
718 GATTC
XhoII 567 AGATCC [GA]^GATC[CT]
633 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI BbsI BbvI
BfaI BpmI BpuAI BsaI BsaJI
BsiYI BslI BsmAI BsmFI BsrDI
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeI MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI StyI Tru9I TseI
Tsp509I TspRI