################################## # NO CUT ENZYMES ( 195 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MroI MseI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AciI 324 GCGG CCGC(-3/-1) AhdI 223 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 270 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 223 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 157 GAGCAC G[AT]GC[AT]^C BalI 287 TGGCCA TGG^CCA BanII 84 GAGCCC G[GA]GC[CT]^C BbsI 220 GAAGAC GAAGAC(2/6) BbvCI 267 CCTCAGC CCTCAGC(-5/-2) BglI 315 GCCATTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 220 GAAGAC GAAGAC(2/6) BseRI 171 CTCCTC GAGGAG(10/8) BsiHKAI 157 GAGCAC G[AT]GC[AT]^C BstBI 151 TTCGAA TT^CGAA BstXI 290 CCAGTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 90 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 160 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 287 TGGCCA [CT]^GGCC[GA] Eam1105I 223 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 154 GAAGAG CTCTTC(1/4) EcoO109I 90 AGGCCCC [GA]G^G[ACGT]CC[CT] HinfI 138 GAATC G^A[ACGT]TC Ksp632I 154 GAAGAG CTCTTC(1/4) MluNI 287 TGGCCA TGG^CCA MscI 287 TGGCCA TGG^CCA MspA1I 242 CAGCTG C[AC]G^C[GT]G NspV 151 TTCGAA TT^CGAA PvuII 242 CAGCTG CAG^CTG SapI 154 GAAGAGC GCTCTTC(1/4) SfuI 151 TTCGAA TT^CGAA TaqI 152 TCGA T^CGA TfiI 138 GAATC G^A[AT]TC TthHB8I 152 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### BmyI 84 GAGCCC G[AGT]GC[ACT]^C 157 GAGCAC BsaJI 8 CCCTGG C^C[ACGT][ACGT]GG 87 CCCAGG Bsp1286I 84 GAGCCC G[AGT]GC[ACT]^C 157 GAGCAC BspMI 63 GCAGGT ACCTGC(4/8) 192 GCAGGT BsrI 225 CCAGT ACTGG(1/-1) 290 CCAGT HphI 66 GGTGA GGTGA(8/7) 181 GGTGA ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AluI BbvI BsiYI BslI BssKI BstNI Cfr13I DdeI EcoRII Fnu4HI HaeIII ItaI MboII MnlI MvaI MwoI NlaIII NlaIV PstI Sau96I ScrFI SfcI TseI TspRI