################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BfrI BlnI BpuAI BsaAI BsaBI BseRI BsgI BsiWI BsmBI BsmI BsoBI BspDI BspHI BsrDI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccIII 181 TCCGGA T^CCGGA AcsI 213 GAATTT [GA]^AATT[CT] AcyI 751 GACGCC G[GA]^CG[CT]C AflIII 596 ACATGT A^C[GA][CT]GT Alw44I 592 GTGCAC G^TGCAC ApaI 548 GGGCCC GGGCC^C ApaLI 592 GTGCAC G^TGCAC ApoI 213 GAATTT [GA]^AATT[CT] AspHI 592 GTGCAC G[AT]GC[AT]^C BcgI 416 GCATCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 905 GTATCC GTATCC(6/5) BclI 240 TGATCA T^GATCA BcnI 294 CCGGG CC^[GC]GG BfaI 205 CTAG C^TAG BglI 298 GCCGTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 800 AGATCT A^GATCT BlpI 816 GCTCAGC GC^T[ACGT]AGC Bpu1102I 816 GCTCAGC GC^T[ACGT]AGC BsaHI 751 GACGCC G[GA]^CG[CT]C BsaI 485 GAGACC GGTCTC(1/5) BsaWI 181 TCCGGA [AT]^CCGG[AT] BseAI 181 TCCGGA T^CCGGA BsiEI 606 CGACCG CG[GA][CT]^CG BsiHKAI 592 GTGCAC G[AT]GC[AT]^C BsmAI 485 GAGAC GTCTC(1/5) BspEI 181 TCCGGA T^CCGGA BspLU11I 596 ACATGT A^CATGT BsrBI 711 CCGCTC CCGCTC(-3/-3) BstXI 74 CCATAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 800 AGATCT [GA]^GATC[CT] CelII 816 GCTCAGC GC^T[ACGT]AGC FbaI 240 TGATCA T^GATCA HaeII 701 AGCGCC [GA]GCGC^[CT] HgaI 751 GACGC GACGC(5/10) Hin1I 751 GACGCC G[GA]^CG[CT]C HphI 668 TCACC GGTGA(8/7) MaeI 205 CTAG C^TAG MflI 800 AGATCT [GA]^GATC[CT] MroI 181 TCCGGA T^CCGGA MseI 34 TTAA T^TAA MspA1I 619 CAGCTG C[AC]G^C[GT]G NaeI 412 GCCGGC GCC^GGC NciI 294 CCGGG CC^[GC]GG NgoMIV 412 GCCGGC G^CCGGC NspI 596 ACATGT [GA]CATG^[CT] PpuMI 824 GGGACCC [GA]G^G[AT]CC[CT] PstI 431 CTGCAG CTGCA^G PvuII 619 CAGCTG CAG^CTG SfcI 431 CTGCAG C^T[GA][CT]AG SfiI 297 GGCCGTGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspI 284 AATATT AAT^ATT StuI 656 AGGCCT AGG^CCT TfiI 336 GATTC G^A[AT]TC Tru9I 34 TTAA T^TAA TspRI 135 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 800 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccII 637 CGCG CG^CG 722 CGCG AfaI 465 GTAC GT^AC 810 GTAC BanII 548 GGGCCC G[GA]GC[CT]^C 733 GAGCCC BsmFI 749 GGGAC GGGAC(10/14) 824 GGGAC Bsp1407I 464 TGTACA T^GTACA 809 TGTACA BspMI 196 ACCTGC ACCTGC(4/8) 433 GCAGGT BsrFI 412 GCCGGC [GA]^CCGG[CT] 608 ACCGGC BsrGI 464 TGTACA T^GTACA 809 TGTACA BstUI 637 CGCG CG^CG 722 CGCG Cfr10I 412 GCCGGC [GA]^CCGG[CT] 608 ACCGGC MaeIII 443 GTAAC ^GT[ACGT]AC 518 GTTAC MboII 419 TCTTC GAAGA(8/7) 422 TCTTC MslI 75 CATAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 456 CATCATAGTG MvnI 637 CGCG CG^CG 722 CGCG PleI 502 GACTC GAGTC(4/5) 925 GACTC RsaI 465 GTAC GT^AC 810 GTAC SspBI 464 TGTACA T^GTACA 809 TGTACA Tsp509I 214 AATT ^AATT 237 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AvaII BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TaqI TseI TthHB8I