##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AlwI AlwNI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BfrI BlnI
BpuAI BsaAI BsaBI BseRI BsgI
BsiWI BsmBI BsmI BsoBI BspDI
BspHI BsrDI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MluNI MscI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StyI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccIII 181 TCCGGA T^CCGGA
AcsI 213 GAATTT [GA]^AATT[CT]
AcyI 751 GACGCC G[GA]^CG[CT]C
AflIII 596 ACATGT A^C[GA][CT]GT
Alw44I 592 GTGCAC G^TGCAC
ApaI 548 GGGCCC GGGCC^C
ApaLI 592 GTGCAC G^TGCAC
ApoI 213 GAATTT [GA]^AATT[CT]
AspHI 592 GTGCAC G[AT]GC[AT]^C
BcgI 416 GCATCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 905 GTATCC GTATCC(6/5)
BclI 240 TGATCA T^GATCA
BcnI 294 CCGGG CC^[GC]GG
BfaI 205 CTAG C^TAG
BglI 298 GCCGTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 800 AGATCT A^GATCT
BlpI 816 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 816 GCTCAGC GC^T[ACGT]AGC
BsaHI 751 GACGCC G[GA]^CG[CT]C
BsaI 485 GAGACC GGTCTC(1/5)
BsaWI 181 TCCGGA [AT]^CCGG[AT]
BseAI 181 TCCGGA T^CCGGA
BsiEI 606 CGACCG CG[GA][CT]^CG
BsiHKAI 592 GTGCAC G[AT]GC[AT]^C
BsmAI 485 GAGAC GTCTC(1/5)
BspEI 181 TCCGGA T^CCGGA
BspLU11I 596 ACATGT A^CATGT
BsrBI 711 CCGCTC CCGCTC(-3/-3)
BstXI 74 CCATAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 800 AGATCT [GA]^GATC[CT]
CelII 816 GCTCAGC GC^T[ACGT]AGC
FbaI 240 TGATCA T^GATCA
HaeII 701 AGCGCC [GA]GCGC^[CT]
HgaI 751 GACGC GACGC(5/10)
Hin1I 751 GACGCC G[GA]^CG[CT]C
HphI 668 TCACC GGTGA(8/7)
MaeI 205 CTAG C^TAG
MflI 800 AGATCT [GA]^GATC[CT]
MroI 181 TCCGGA T^CCGGA
MseI 34 TTAA T^TAA
MspA1I 619 CAGCTG C[AC]G^C[GT]G
NaeI 412 GCCGGC GCC^GGC
NciI 294 CCGGG CC^[GC]GG
NgoMIV 412 GCCGGC G^CCGGC
NspI 596 ACATGT [GA]CATG^[CT]
PpuMI 824 GGGACCC [GA]G^G[AT]CC[CT]
PstI 431 CTGCAG CTGCA^G
PvuII 619 CAGCTG CAG^CTG
SfcI 431 CTGCAG C^T[GA][CT]AG
SfiI 297 GGCCGTGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 284 AATATT AAT^ATT
StuI 656 AGGCCT AGG^CCT
TfiI 336 GATTC G^A[AT]TC
Tru9I 34 TTAA T^TAA
TspRI 135 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 800 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 637 CGCG CG^CG
722 CGCG
AfaI 465 GTAC GT^AC
810 GTAC
BanII 548 GGGCCC G[GA]GC[CT]^C
733 GAGCCC
BsmFI 749 GGGAC GGGAC(10/14)
824 GGGAC
Bsp1407I 464 TGTACA T^GTACA
809 TGTACA
BspMI 196 ACCTGC ACCTGC(4/8)
433 GCAGGT
BsrFI 412 GCCGGC [GA]^CCGG[CT]
608 ACCGGC
BsrGI 464 TGTACA T^GTACA
809 TGTACA
BstUI 637 CGCG CG^CG
722 CGCG
Cfr10I 412 GCCGGC [GA]^CCGG[CT]
608 ACCGGC
MaeIII 443 GTAAC ^GT[ACGT]AC
518 GTTAC
MboII 419 TCTTC GAAGA(8/7)
422 TCTTC
MslI 75 CATAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
456 CATCATAGTG
MvnI 637 CGCG CG^CG
722 CGCG
PleI 502 GACTC GAGTC(4/5)
925 GACTC
RsaI 465 GTAC GT^AC
810 GTAC
SspBI 464 TGTACA T^GTACA
809 TGTACA
Tsp509I 214 AATT ^AATT
237 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI TthHB8I