Restriction map for pF1KE0491
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        AlwI        AlwNI       AocI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BfrI        BlnI        
BpuAI       BsaAI       BsaBI       BseRI       BsgI        
BsiWI       BsmBI       BsmI        BsoBI       BspDI       
BspHI       BsrDI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MscI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    StyI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccIII        181  TCCGGA               T^CCGGA
AcsI          213  GAATTT               [GA]^AATT[CT]
AcyI          751  GACGCC               G[GA]^CG[CT]C
AflIII        596  ACATGT               A^C[GA][CT]GT
Alw44I        592  GTGCAC               G^TGCAC
ApaI          548  GGGCCC               GGGCC^C
ApaLI         592  GTGCAC               G^TGCAC
ApoI          213  GAATTT               [GA]^AATT[CT]
AspHI         592  GTGCAC               G[AT]GC[AT]^C
BcgI          416  GCATCTTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         905  GTATCC               GTATCC(6/5)
BclI          240  TGATCA               T^GATCA
BcnI          294  CCGGG                CC^[GC]GG
BfaI          205  CTAG                 C^TAG
BglI          298  GCCGTGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         800  AGATCT               A^GATCT
BlpI          816  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      816  GCTCAGC              GC^T[ACGT]AGC
BsaHI         751  GACGCC               G[GA]^CG[CT]C
BsaI          485  GAGACC               GGTCTC(1/5)
BsaWI         181  TCCGGA               [AT]^CCGG[AT]
BseAI         181  TCCGGA               T^CCGGA
BsiEI         606  CGACCG               CG[GA][CT]^CG
BsiHKAI       592  GTGCAC               G[AT]GC[AT]^C
BsmAI         485  GAGAC                GTCTC(1/5)
BspEI         181  TCCGGA               T^CCGGA
BspLU11I      596  ACATGT               A^CATGT
BsrBI         711  CCGCTC               CCGCTC(-3/-3)
BstXI          74  CCATAGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         800  AGATCT               [GA]^GATC[CT]
CelII         816  GCTCAGC              GC^T[ACGT]AGC
FbaI          240  TGATCA               T^GATCA
HaeII         701  AGCGCC               [GA]GCGC^[CT]
HgaI          751  GACGC                GACGC(5/10)
Hin1I         751  GACGCC               G[GA]^CG[CT]C
HphI          668  TCACC                GGTGA(8/7)
MaeI          205  CTAG                 C^TAG
MflI          800  AGATCT               [GA]^GATC[CT]
MroI          181  TCCGGA               T^CCGGA
MseI           34  TTAA                 T^TAA
MspA1I        619  CAGCTG               C[AC]G^C[GT]G
NaeI          412  GCCGGC               GCC^GGC
NciI          294  CCGGG                CC^[GC]GG
NgoMIV        412  GCCGGC               G^CCGGC
NspI          596  ACATGT               [GA]CATG^[CT]
PpuMI         824  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          431  CTGCAG               CTGCA^G
PvuII         619  CAGCTG               CAG^CTG
SfcI          431  CTGCAG               C^T[GA][CT]AG
SfiI          297  GGCCGTGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI          284  AATATT               AAT^ATT
StuI          656  AGGCCT               AGG^CCT
TfiI          336  GATTC                G^A[AT]TC
Tru9I          34  TTAA                 T^TAA
TspRI         135  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         800  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccII         637  CGCG                 CG^CG
              722  CGCG                
AfaI          465  GTAC                 GT^AC
              810  GTAC                
BanII         548  GGGCCC               G[GA]GC[CT]^C
              733  GAGCCC              
BsmFI         749  GGGAC                GGGAC(10/14)
              824  GGGAC               
Bsp1407I      464  TGTACA               T^GTACA
              809  TGTACA              
BspMI         196  ACCTGC               ACCTGC(4/8)
              433  GCAGGT              
BsrFI         412  GCCGGC               [GA]^CCGG[CT]
              608  ACCGGC              
BsrGI         464  TGTACA               T^GTACA
              809  TGTACA              
BstUI         637  CGCG                 CG^CG
              722  CGCG                
Cfr10I        412  GCCGGC               [GA]^CCGG[CT]
              608  ACCGGC              
MaeIII        443  GTAAC                ^GT[ACGT]AC
              518  GTTAC               
MboII         419  TCTTC                GAAGA(8/7)
              422  TCTTC               
MslI           75  CATAGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              456  CATCATAGTG          
MvnI          637  CGCG                 CG^CG
              722  CGCG                
PleI          502  GACTC                GAGTC(4/5)
              925  GACTC               
RsaI          465  GTAC                 GT^AC
              810  GTAC                
SspBI         464  TGTACA               T^GTACA
              809  TGTACA              
Tsp509I       214  AATT                 ^AATT
              237  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BmyI        
BpmI        BsaJI       BsiYI       BslI        Bsp1286I    
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        MboI        MnlI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TseI        TthHB8I     
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