Restriction map for
pF1KE0407
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HphI KasI KpnI Ksp632I
MaeII MamI MflI MluI MluNI
MroI MscI NarI NciI NcoI
NdeI NheI NotI NruI NspI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 70 CGCG CG^CG
AfaI 334 GTAC GT^AC
AspI 373 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvI 706 GCAGC GCAGC(8/12)
BfaI 385 CTAG C^TAG
BglI 66 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 622 GAGACC GGTCTC(1/5)
BseRI 44 GAGGAG GAGGAG(10/8)
BsiYI 189 CCATGCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 189 CCATGCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrFI 84 GCCGGC [GA]^CCGG[CT]
BstUI 70 CGCG CG^CG
Cfr10I 84 GCCGGC [GA]^CCGG[CT]
DraII 64 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 69 CCGCGG C^C[GA][CT]GG
EaeI 139 CGGCCA [CT]^GGCC[GA]
EcoO109I 64 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 505 GATATC GAT^ATC
HapII 85 CCGG C^CGG
HgaI 640 GACGC GACGC(5/10)
HincII 258 GTTAAC GT[CT]^[GA]AC
HindII 258 GTTAAC GT[CT]^[GA]AC
HpaI 258 GTTAAC GTT^AAC
HpaII 85 CCGG C^CGG
KspI 69 CCGCGG CCGC^GG
MaeI 385 CTAG C^TAG
MboII 104 TCTTC GAAGA(8/7)
MfeI 245 CAATTG C^AATTG
MslI 352 CATTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 69 CCGCGG C[AC]G^C[GT]G
MspI 85 CCGG C^CGG
MunI 245 CAATTG C^AATTG
MvnI 70 CGCG CG^CG
NaeI 84 GCCGGC GCC^GGC
NgoMIV 84 GCCGGC G^CCGGC
NlaIV 65 GGCCCC GG[ACGT]^[ACGT]CC
PflFI 373 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 189 CCATGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 334 GTAC GT^AC
SacII 69 CCGCGG CCGC^GG
SfaNI 358 GATGC GCATC(5/9)
SfcI 575 CTACAG C^T[GA][CT]AG
TaqI 480 TCGA T^CGA
TseI 706 GCAGC G^C[AT]GC
Tsp45I 307 GTCAC ^GT[GC]AC
Tth111I 373 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 480 TCGA T^CGA
Van91I 189 CCATGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 397 GAATTT [GA]^AATT[CT]
580 GAATTT
ApoI 397 GAATTT [GA]^AATT[CT]
580 GAATTT
BciVI 285 GGATAC GTATCC(6/5)
594 GGATAC
BsmAI 622 GAGAC GTCTC(1/5)
688 GAGAC
BstF5I 381 CATCC GGATG(2/0)
357 GGATG
Cac8I 71 GCGGGC GC[ACGT]^[ACGT]GC
84 GCCGGC
Cfr13I 65 GGCCC G^G[ACGT]CC
87 GGCCC
DdeI 409 CTGAG C^T[ACGT]AG
513 CTTAG
DpnII 49 GATC ^GATC
547 GATC
EcoT14I 36 CCAAGG C^C[AT][AT]GG
119 CCAAGG
EcoT22I 191 ATGCAT ATGCA^T
466 ATGCAT
FauI 68 CCCGC CCCGC(4/6)
71 GCGGG
FokI 381 CATCC GGATG(9/13)
357 GGATG
MaeIII 307 GTCAC ^GT[ACGT]AC
529 GTAAC
MboI 49 GATC ^GATC
547 GATC
MnlI 44 GAGG CCTC(7/6)
124 GAGG
NdeII 49 GATC ^GATC
547 GATC
NsiI 191 ATGCAT ATGCA^T
466 ATGCAT
Sau3AI 49 GATC ^GATC
547 GATC
Sau96I 65 GGCCC G^G[ACGT]CC
87 GGCCC
SmlI 311 CTCAAG C^T[CT][GA]AG
685 CTTGAG
StyI 36 CCAAGG C^C[AT][AT]GG
119 CCAAGG
TspRI 143 CAGTG CA[GC]TG[ACGT][ACGT]^
665 CACTG
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AluI BsaJI BsrI BssKI
BstNI EcoRII Fnu4HI HaeIII HinfI
ItaI MseI MvaI MwoI NlaIII
ScrFI TfiI Tru9I Tsp509I