################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MflI MluI MnlI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AciI 11 CCGC CCGC(-3/-1) AlwNI 74 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 264 GGACC G^G[AT]CC BanII 61 GGGCTC G[GA]GC[CT]^C BfaI 136 CTAG C^TAG BmyI 61 GGGCTC G[AGT]GC[ACT]^C BsaI 6 GGTCTC GGTCTC(1/5) BsgI 241 GTGCAG GTGCAG(16/14) BsmAI 7 GTCTC GTCTC(1/5) Bsp1286I 61 GGGCTC G[AGT]GC[ACT]^C BspMI 91 ACCTGC ACCTGC(4/8) DpnII 186 GATC ^GATC EcoRV 211 GATATC GAT^ATC EcoT14I 206 CCAAGG C^C[AT][AT]GG HphI 155 TCACC GGTGA(8/7) MaeI 136 CTAG C^TAG MboI 186 GATC ^GATC MseI 101 TTAA T^TAA MslI 17 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 41 CAGCTG C[AC]G^C[GT]G NdeII 186 GATC ^GATC NlaIII 252 CATG CATG^ PvuII 41 CAGCTG CAG^CTG Sau3AI 186 GATC ^GATC SfcI 144 CTACAG C^T[GA][CT]AG StyI 206 CCAAGG C^C[AT][AT]GG Tru9I 101 TTAA T^TAA TspRI 159 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BalI 107 TGGCCA TGG^CCA 203 TGGCCA BsmFI 82 GTCCC GGGAC(10/14) 170 GTCCC BsrI 201 ACTGG ACTGG(1/-1) 158 CCAGT BssKI 57 CCAGG ^CC[ACGT]GG 105 CCTGG BstNI 57 CCAGG CC^[AT]GG 105 CCTGG Cac8I 63 GCTCGC GC[ACGT]^[ACGT]GC 72 GCCAGC Cfr13I 70 GGGCC G^G[ACGT]CC 264 GGACC EaeI 107 TGGCCA [CT]^GGCC[GA] 203 TGGCCA EcoRII 57 CCAGG ^CC[AT]GG 105 CCTGG HinfI 152 GAATC G^A[ACGT]TC 247 GATTC MluNI 107 TGGCCA TGG^CCA 203 TGGCCA MscI 107 TGGCCA TGG^CCA 203 TGGCCA MvaI 57 CCAGG CC^[AT]GG 105 CCTGG MwoI 63 GCTCGCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 67 GCTGGGCCAGC Sau96I 70 GGGCC G^G[ACGT]CC 264 GGACC ScrFI 57 CCAGG CC^[ACGT]GG 105 CCTGG TfiI 152 GAATC G^A[AT]TC 247 GATTC XcmI 57 CCAGGGGCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 251 CCATGAAGTATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AluI BbvI BsaJI Fnu4HI HaeIII ItaI MboII TseI