Restriction map for pF1KB6737
##################################
# NO CUT ENZYMES (  204 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeII       MaeIII      
MamI        MfeI        MflI        MluI        MnlI        
MroI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tsp45I      Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AciI           11  CCGC                 CCGC(-3/-1)
AlwNI          74  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         264  GGACC                G^G[AT]CC
BanII          61  GGGCTC               G[GA]GC[CT]^C
BfaI          136  CTAG                 C^TAG
BmyI           61  GGGCTC               G[AGT]GC[ACT]^C
BsaI            6  GGTCTC               GGTCTC(1/5)
BsgI          241  GTGCAG               GTGCAG(16/14)
BsmAI           7  GTCTC                GTCTC(1/5)
Bsp1286I       61  GGGCTC               G[AGT]GC[ACT]^C
BspMI          91  ACCTGC               ACCTGC(4/8)
DpnII         186  GATC                 ^GATC
EcoRV         211  GATATC               GAT^ATC
EcoT14I       206  CCAAGG               C^C[AT][AT]GG
HphI          155  TCACC                GGTGA(8/7)
MaeI          136  CTAG                 C^TAG
MboI          186  GATC                 ^GATC
MseI          101  TTAA                 T^TAA
MslI           17  CACTTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         41  CAGCTG               C[AC]G^C[GT]G
NdeII         186  GATC                 ^GATC
NlaIII        252  CATG                 CATG^
PvuII          41  CAGCTG               CAG^CTG
Sau3AI        186  GATC                 ^GATC
SfcI          144  CTACAG               C^T[GA][CT]AG
StyI          206  CCAAGG               C^C[AT][AT]GG
Tru9I         101  TTAA                 T^TAA
TspRI         159  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BalI          107  TGGCCA               TGG^CCA
              203  TGGCCA              
BsmFI          82  GTCCC                GGGAC(10/14)
              170  GTCCC               
BsrI          201  ACTGG                ACTGG(1/-1)
              158  CCAGT               
BssKI          57  CCAGG                ^CC[ACGT]GG
              105  CCTGG               
BstNI          57  CCAGG                CC^[AT]GG
              105  CCTGG               
Cac8I          63  GCTCGC               GC[ACGT]^[ACGT]GC
               72  GCCAGC              
Cfr13I         70  GGGCC                G^G[ACGT]CC
              264  GGACC               
EaeI          107  TGGCCA               [CT]^GGCC[GA]
              203  TGGCCA              
EcoRII         57  CCAGG                ^CC[AT]GG
              105  CCTGG               
HinfI         152  GAATC                G^A[ACGT]TC
              247  GATTC               
MluNI         107  TGGCCA               TGG^CCA
              203  TGGCCA              
MscI          107  TGGCCA               TGG^CCA
              203  TGGCCA              
MvaI           57  CCAGG                CC^[AT]GG
              105  CCTGG               
MwoI           63  GCTCGCTGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               67  GCTGGGCCAGC         
Sau96I         70  GGGCC                G^G[ACGT]CC
              264  GGACC               
ScrFI          57  CCAGG                CC^[ACGT]GG
              105  CCTGG               
TfiI          152  GAATC                G^A[AT]TC
              247  GATTC               
XcmI           57  CCAGGGGCTCGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              251  CCATGAAGTATCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AluI        BbvI        BsaJI       Fnu4HI      HaeIII      
ItaI        MboII       TseI        
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