################################## # NO CUT ENZYMES ( 198 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BlnI BmyI BpmI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AlwNI 136 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 243 GGTCC G^G[AT]CC BbsI 288 GAAGAC GAAGAC(2/6) BclI 155 TGATCA T^GATCA BlpI 67 GCTCAGC GC^T[ACGT]AGC Bpu1102I 67 GCTCAGC GC^T[ACGT]AGC BpuAI 288 GAAGAC GAAGAC(2/6) BsmAI 7 GTCTC GTCTC(1/5) BspHI 35 TCATGA T^CATGA BspMI 167 GCAGGT ACCTGC(4/8) BsrI 204 ACTGG ACTGG(1/-1) Cac8I 26 GCCTGC GC[ACGT]^[ACGT]GC CelII 67 GCTCAGC GC^T[ACGT]AGC DdeI 68 CTCAG C^T[ACGT]AG EarI 144 GAAGAG CTCTTC(1/4) EcoNI 131 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 155 TGATCA T^GATCA HaeIII 268 GGCC GG^CC HapII 270 CCGG C^CGG HpaII 270 CCGG C^CGG HphI 158 TCACC GGTGA(8/7) Ksp632I 144 GAAGAG CTCTTC(1/4) MaeII 86 ACGT A^CGT MnlI 138 GAGG CCTC(7/6) MspI 270 CCGG C^CGG NlaIII 36 CATG CATG^ NlaIV 242 GGGTCC GG[ACGT]^[ACGT]CC PstI 11 CTGCAG CTGCA^G RcaI 35 TCATGA T^CATGA SapI 144 GAAGAGC GCTCTTC(1/4) SfaNI 76 GATGC GCATC(5/9) SfcI 11 CTGCAG C^T[GA][CT]AG Tsp509I 250 AATT ^AATT TspRI 14 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 229 CCAAAGGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BglII 125 AGATCT A^GATCT 215 AGATCT BsiYI 131 CCTTGCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 161 CCACCAGCAGG BslI 131 CCTTGCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 161 CCACCAGCAGG BssKI 57 CCAGG ^CC[ACGT]GG 264 CCTGG BstNI 57 CCAGG CC^[AT]GG 264 CCTGG BstYI 125 AGATCT [GA]^GATC[CT] 215 AGATCT Cfr13I 243 GGTCC G^G[ACGT]CC 267 GGGCC EcoRII 57 CCAGG ^CC[AT]GG 264 CCTGG MflI 125 AGATCT [GA]^GATC[CT] 215 AGATCT MvaI 57 CCAGG CC^[AT]GG 264 CCTGG Sau96I 243 GGTCC G^G[ACGT]CC 267 GGGCC ScrFI 57 CCAGG CC^[ACGT]GG 264 CCTGG XhoII 125 AGATCT [GA]^GATC[CT] 215 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AluI BbvI BsaJI BsmFI DpnII Fnu4HI ItaI MboI MboII NdeII Sau3AI TseI