Restriction map for pF1KB6741
##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BcnI        BfaI        BfrI        BglI        
BlnI        BmyI        BpmI        BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FokI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        KspI        MaeI        MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AlwNI         136  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         243  GGTCC                G^G[AT]CC
BbsI          288  GAAGAC               GAAGAC(2/6)
BclI          155  TGATCA               T^GATCA
BlpI           67  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       67  GCTCAGC              GC^T[ACGT]AGC
BpuAI         288  GAAGAC               GAAGAC(2/6)
BsmAI           7  GTCTC                GTCTC(1/5)
BspHI          35  TCATGA               T^CATGA
BspMI         167  GCAGGT               ACCTGC(4/8)
BsrI          204  ACTGG                ACTGG(1/-1)
Cac8I          26  GCCTGC               GC[ACGT]^[ACGT]GC
CelII          67  GCTCAGC              GC^T[ACGT]AGC
DdeI           68  CTCAG                C^T[ACGT]AG
EarI          144  GAAGAG               CTCTTC(1/4)
EcoNI         131  CCTTGCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          155  TGATCA               T^GATCA
HaeIII        268  GGCC                 GG^CC
HapII         270  CCGG                 C^CGG
HpaII         270  CCGG                 C^CGG
HphI          158  TCACC                GGTGA(8/7)
Ksp632I       144  GAAGAG               CTCTTC(1/4)
MaeII          86  ACGT                 A^CGT
MnlI          138  GAGG                 CCTC(7/6)
MspI          270  CCGG                 C^CGG
NlaIII         36  CATG                 CATG^
NlaIV         242  GGGTCC               GG[ACGT]^[ACGT]CC
PstI           11  CTGCAG               CTGCA^G
RcaI           35  TCATGA               T^CATGA
SapI          144  GAAGAGC              GCTCTTC(1/4)
SfaNI          76  GATGC                GCATC(5/9)
SfcI           11  CTGCAG               C^T[GA][CT]AG
Tsp509I       250  AATT                 ^AATT
TspRI          14  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI          229  CCAAAGGAGAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BglII         125  AGATCT               A^GATCT
              215  AGATCT              
BsiYI         131  CCTTGCAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              161  CCACCAGCAGG         
BslI          131  CCTTGCAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              161  CCACCAGCAGG         
BssKI          57  CCAGG                ^CC[ACGT]GG
              264  CCTGG               
BstNI          57  CCAGG                CC^[AT]GG
              264  CCTGG               
BstYI         125  AGATCT               [GA]^GATC[CT]
              215  AGATCT              
Cfr13I        243  GGTCC                G^G[ACGT]CC
              267  GGGCC               
EcoRII         57  CCAGG                ^CC[AT]GG
              264  CCTGG               
MflI          125  AGATCT               [GA]^GATC[CT]
              215  AGATCT              
MvaI           57  CCAGG                CC^[AT]GG
              264  CCTGG               
Sau96I        243  GGTCC                G^G[ACGT]CC
              267  GGGCC               
ScrFI          57  CCAGG                CC^[ACGT]GG
              264  CCTGG               
XhoII         125  AGATCT               [GA]^GATC[CT]
              215  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsmFI       DpnII       
Fnu4HI      ItaI        MboI        MboII       NdeII       
Sau3AI      TseI        
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