##################################
# NO CUT ENZYMES (  200 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiWI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HaeIII      HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MunI        
MvnI        NarI        NdeI        NdeII       NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tru9I       Tsp45I      
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AciI           92  CCGC                 CCGC(-3/-1)
AcsI          132  GAATTT               [GA]^AATT[CT]
ApoI          132  GAATTT               [GA]^AATT[CT]
AspHI         168  GTGCTC               G[AT]GC[AT]^C
AvaII         240  GGTCC                G^G[AT]CC
BmyI          168  GTGCTC               G[AGT]GC[ACT]^C
BpmI          262  CTGGAG               CTGGAG(16/14)
BsaI            6  GGTCTC               GGTCTC(1/5)
BsaJI          41  CCATGG               C^C[ACGT][ACGT]GG
BsgI           36  CTGCAC               GTGCAG(16/14)
BsiEI          89  CGACCG               CG[GA][CT]^CG
BsiHKAI       168  GTGCTC               G[AT]GC[AT]^C
BsiYI         225  CCCCAGTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          225  CCCCAGTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI         154  GAGACG               CGTCTC(1/5)
Bsp1286I      168  GTGCTC               G[AGT]GC[ACT]^C
BsrFI         206  GCCGGC               [GA]^CCGG[CT]
BstNI         261  CCTGG                CC^[AT]GG
Cfr10I        206  GCCGGC               [GA]^CCGG[CT]
Cfr13I        240  GGTCC                G^G[ACGT]CC
DdeI          268  CTGAG                C^T[ACGT]AG
DsaI           41  CCATGG               C^C[GA][CT]GG
EcoRII        261  CCTGG                ^CC[AT]GG
EcoT14I        41  CCATGG               C^C[AT][AT]GG
HinfI         123  GATTC                G^A[ACGT]TC
MboII         188  TCTTC                GAAGA(8/7)
MvaI          261  CCTGG                CC^[AT]GG
NaeI          206  GCCGGC               GCC^GGC
NcoI           41  CCATGG               C^CATGG
NgoMIV        206  GCCGGC               G^CCGGC
NlaIII         42  CATG                 CATG^
NlaIV         239  GGGTCC               GG[ACGT]^[ACGT]CC
Sau96I        240  GGTCC                G^G[ACGT]CC
SfaNI          67  GCATC                GCATC(5/9)
StyI           41  CCATGG               C^C[AT][AT]GG
TfiI          123  GATTC                G^A[AT]TC
Tsp509I       133  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BcnI          116  CCCGG                CC^[GC]GG
              178  CCCGG               
BseRI          31  CTCCTC               GAGGAG(10/8)
              232  GAGGAG              
BsmAI         154  GAGAC                GTCTC(1/5)
                7  GTCTC               
BspMI           3  GCAGGT               ACCTGC(4/8)
              210  GCAGGT              
BstAPI         67  GCATCACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              161  GCAGCCAGTGC         
Cac8I          94  GCCTGC               GC[ACGT]^[ACGT]GC
              206  GCCGGC              
NciI          116  CCCGG                CC^[GC]GG
              178  CCCGG               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BbvI        BsrI        BssKI       Fnu4HI      
HapII       HpaII       ItaI        MnlI        MspI        
MwoI        ScrFI       TseI        TspRI