Restriction map for
pF1KB8567
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BcgI BclI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstNI BstPI BstUI BstYI BstZ17I
CelII Cfr10I CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRII EcoT14I EcoT22I FauI FbaI
FseI FspI HpaI KasI KpnI
KspI MfeI MflI MluI MroI
MunI MvaI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI StyI SwaI Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 14 GACGCC G[GA]^CG[CT]C
AflII 807 CTTAAG C^TTAAG
AflIII 563 ACATGT A^C[GA][CT]GT
AlwNI 926 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 283 CCTGAGG CC^T[ACGT]AGG
AspI 21 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1040 TGGCCA TGG^CCA
BbsI 691 GAAGAC GAAGAC(2/6)
BciVI 1000 GGATAC GTATCC(6/5)
BfrI 807 CTTAAG C^TTAAG
BpmI 433 CTGGAG CTGGAG(16/14)
BpuAI 691 GAAGAC GAAGAC(2/6)
BsaBI 409 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 14 GACGCC G[GA]^CG[CT]C
BsaJI 827 CCGGGG C^C[ACGT][ACGT]GG
BseRI 667 GAGGAG GAGGAG(10/8)
Bsp1407I 560 TGTACA T^GTACA
BspDI 584 ATCGAT AT^CGAT
BspLU11I 563 ACATGT A^CATGT
BsrGI 560 TGTACA T^GTACA
BstAPI 925 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 283 CCTGAGG CC^T[ACGT]AGG
CfoI 4 GCGC GCG^C
ClaI 584 ATCGAT AT^CGAT
DraII 267 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 1040 TGGCCA [CT]^GGCC[GA]
Eco81I 283 CCTGAGG CC^T[ACGT]AGG
EcoO109I 267 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 409 GATATC GAT^ATC
HaeII 3 GGCGCT [GA]GCGC^[CT]
HhaI 4 GCGC GCG^C
Hin1I 14 GACGCC G[GA]^CG[CT]C
HinP1I 4 GCGC G^CGC
HincII 202 GTCAAC GT[CT]^[GA]AC
HindII 202 GTCAAC GT[CT]^[GA]AC
HphI 1053 GGTGA GGTGA(8/7)
MaeII 401 ACGT A^CGT
MamI 409 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1040 TGGCCA TGG^CCA
MscI 1040 TGGCCA TGG^CCA
MslI 501 CATCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 21 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 267 GGGTCCT [GA]G^G[AT]CC[CT]
SmlI 807 CTTAAG C^T[CT][GA]AG
SphI 491 GCATGC GCATG^C
SspBI 560 TGTACA T^GTACA
TfiI 309 GAATC G^A[AT]TC
Tth111I 21 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 860 CCAAGTATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 772 AAATTT [GA]^AATT[CT]
1078 AAATTC
AlwI 1134 GATCC GGATC(4/5)
582 GGATC
ApoI 772 AAATTT [GA]^AATT[CT]
1078 AAATTC
AvaII 268 GGTCC G^G[AT]CC
1190 GGTCC
BbvCI 382 CCTCAGC CCTCAGC(-5/-2)
1138 CCTCAGC
BcnI 611 CCCGG CC^[GC]GG
827 CCGGG
BfaI 505 CTAG C^TAG
958 CTAG
BssKI 611 CCCGG ^CC[ACGT]GG
827 CCGGG
EarI 304 GAAGAG CTCTTC(1/4)
473 GAAGAG
HaeIII 784 GGCC GG^CC
1041 GGCC
HapII 612 CCGG C^CGG
827 CCGG
HgaI 14 GACGC GACGC(5/10)
488 GACGC
HindIII 320 AAGCTT A^AGCTT
619 AAGCTT
HpaII 612 CCGG C^CGG
827 CCGG
Ksp632I 304 GAAGAG CTCTTC(1/4)
473 GAAGAG
MaeI 505 CTAG C^TAG
958 CTAG
MspI 612 CCGG C^CGG
827 CCGG
NciI 611 CCCGG CC^[GC]GG
827 CCGGG
NlaIV 258 GGCTCC GG[ACGT]^[ACGT]CC
267 GGGTCC
NspI 491 GCATGC [GA]CATG^[CT]
563 ACATGT
PstI 390 CTGCAG CTGCA^G
547 CTGCAG
ScrFI 611 CCCGG CC^[ACGT]GG
827 CCGGG
SfaNI 331 GATGC GCATC(5/9)
817 GCATC
SfcI 390 CTGCAG C^T[GA][CT]AG
547 CTGCAG
SspI 43 AATATT AAT^ATT
685 AATATT
Tsp45I 82 GTGAC ^GT[GC]AC
395 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AfaI AluI BbvI BsiYI BslI
BsmAI BsrI BstF5I BstXI Cac8I
Cfr13I DdeI DpnII Fnu4HI FokI
HinfI ItaI MaeIII MboI MboII
MnlI MseI MspA1I MwoI NdeII
NlaIII PleI PvuII RsaI Sau3AI
Sau96I TaqI Tru9I TseI Tsp509I
TspRI TthHB8I