Restriction map for
pF1KB6363
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BbrPI
BbvCI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
ClaI CpoI DraI DraIII DrdI
DsaI Eam1105I Eco47III EcoNI EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HgaI HindIII HpaI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NotI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI StyI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 394 CGCG CG^CG
AcyI 226 GGCGCC G[GA]^CG[CT]C
ApaI 308 GGGCCC GGGCC^C
Asp700I 567 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 713 TGGCCA TGG^CCA
BamHI 98 GGATCC G^GATCC
BanI 226 GGCGCC G^G[CT][GA]CC
BbeI 226 GGCGCC GGCGC^C
BcgI 686 GCATCATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 144 CTCCAG CTGGAG(16/14)
BsaAI 424 TACGTG [CT]AC^GT[GA]
BsaHI 226 GGCGCC G[GA]^CG[CT]C
BseRI 141 CTCCTC GAGGAG(10/8)
BsiEI 302 CGGCCG CG[GA][CT]^CG
BsmAI 377 GAGAC GTCTC(1/5)
BspHI 491 TCATGA T^CATGA
BsrDI 288 CATTGC GCAATG(2/0)
BsrFI 758 GCCGGC [GA]^CCGG[CT]
BstEII 507 GGTCACC G^GT[ACGT]ACC
BstPI 507 GGTCACC G^GT[ACGT]ACC
BstUI 394 CGCG CG^CG
BstYI 98 GGATCC [GA]^GATC[CT]
Cfr10I 758 GCCGGC [GA]^CCGG[CT]
EagI 302 CGGCCG C^GGCCG
EclXI 302 CGGCCG C^GGCCG
Eco52I 302 CGGCCG C^GGCCG
EcoO65I 507 GGTCACC G^GT[ACGT]ACC
HaeII 226 GGCGCC [GA]GCGC^[CT]
Hin1I 226 GGCGCC G[GA]^CG[CT]C
HincII 232 GTCAAC GT[CT]^[GA]AC
HindII 232 GTCAAC GT[CT]^[GA]AC
HinfI 495 GAATC G^A[ACGT]TC
KasI 226 GGCGCC G^GCGCC
MflI 98 GGATCC [GA]^GATC[CT]
MluNI 713 TGGCCA TGG^CCA
MscI 713 TGGCCA TGG^CCA
MslI 177 CATCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 243 CCGCTG C[AC]G^C[GT]G
MvnI 394 CGCG CG^CG
NaeI 758 GCCGGC GCC^GGC
NarI 226 GGCGCC GG^CGCC
NgoMIV 758 GCCGGC G^CCGGC
NruI 393 TCGCGA TCG^CGA
RcaI 491 TCATGA T^CATGA
SbfI 405 CCTGCAGG CCTGCA^GG
SfoI 226 GGCGCC GGC^GCC
Sse8387I 405 CCTGCAGG CCTGCA^GG
TfiI 495 GAATC G^A[AT]TC
TspRI 74 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 98 GGATCC [GA]^GATC[CT]
XmnI 567 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 502 CCTGAGG CC^T[ACGT]AGG
616 CCTGAGG
BbsI 775 GAAGAC GAAGAC(2/6)
172 GTCTTC
BcnI 305 CCGGG CC^[GC]GG
586 CCCGG
BlpI 5 GCTCAGC GC^T[ACGT]AGC
484 GCTGAGC
Bpu1102I 5 GCTCAGC GC^T[ACGT]AGC
484 GCTGAGC
BpuAI 775 GAAGAC GAAGAC(2/6)
172 GTCTTC
BsgI 427 GTGCAG GTGCAG(16/14)
658 GTGCAG
BsmFI 419 GGGAC GGGAC(10/14)
790 GTCCC
BsrI 470 ACTGG ACTGG(1/-1)
623 ACTGG
Bsu36I 502 CCTGAGG CC^T[ACGT]AGG
616 CCTGAGG
CelII 5 GCTCAGC GC^T[ACGT]AGC
484 GCTGAGC
CfoI 71 GCGC GCG^C
227 GCGC
Eco81I 502 CCTGAGG CC^T[ACGT]AGG
616 CCTGAGG
HhaI 71 GCGC GCG^C
227 GCGC
HinP1I 71 GCGC G^CGC
227 GCGC
MaeII 425 ACGT A^CGT
764 ACGT
NciI 305 CCGGG CC^[GC]GG
586 CCCGG
PpuMI 595 AGGACCC [GA]G^G[AT]CC[CT]
788 AGGTCCC
PstI 406 CTGCAG CTGCA^G
670 CTGCAG
TaqI 375 TCGA T^CGA
722 TCGA
TthHB8I 375 TCGA T^CGA
722 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp45I