Restriction map for
pF1KB8597
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AvaI
AvaII AviII AvrII BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DrdI DsaI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MluI MroI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 55 AAATTT [GA]^AATT[CT]
AfaI 585 GTAC GT^AC
AflIII 608 ACATGT A^C[GA][CT]GT
AhdI 70 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 715 GGGCCC GGGCC^C
ApoI 55 AAATTT [GA]^AATT[CT]
AspEI 70 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 651 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 595 TGGCCA TGG^CCA
BamHI 340 GGATCC G^GATCC
BclI 89 TGATCA T^GATCA
BlpI 426 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 426 GCTTAGC GC^T[ACGT]AGC
BspLU11I 608 ACATGT A^CATGT
BssSI 510 CTCGTG CACGAG(-5/-1)
BstXI 228 CCAGTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 340 GGATCC [GA]^GATC[CT]
CelII 426 GCTTAGC GC^T[ACGT]AGC
DraI 58 TTTAAA TTT^AAA
DraII 715 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 507 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 595 TGGCCA [CT]^GGCC[GA]
Eam1105I 70 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 715 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 546 CCAAGG C^C[AT][AT]GG
FauI 99 CCCGC CCCGC(4/6)
FbaI 89 TGATCA T^GATCA
HphI 370 TCACC GGTGA(8/7)
MflI 340 GGATCC [GA]^GATC[CT]
MluNI 595 TGGCCA TGG^CCA
MscI 595 TGGCCA TGG^CCA
MspA1I 522 CAGCGG C[AC]G^C[GT]G
NspI 608 ACATGT [GA]CATG^[CT]
PflFI 651 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 700 CTGCAG CTGCA^G
RsaI 585 GTAC GT^AC
SacI 634 GAGCTC GAGCT^C
SfaNI 532 GCATC GCATC(5/9)
SfcI 700 CTGCAG C^T[GA][CT]AG
StyI 546 CCAAGG C^C[AT][AT]GG
TspRI 374 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 651 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 340 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 100 CCGC CCGC(-3/-1)
524 GCGG
AlwI 341 GATCC GGATC(4/5)
340 GGATC
AspHI 304 GTGCTC G[AT]GC[AT]^C
634 GAGCTC
BcnI 255 CCGGG CC^[GC]GG
300 CCCGG
BpmI 519 CTCCAG CTGGAG(16/14)
567 CTCCAG
BsiHKAI 304 GTGCTC G[AT]GC[AT]^C
634 GAGCTC
BsiYI 97 CCCCCGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
328 CCGACCATTGG
BslI 97 CCCCCGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
328 CCGACCATTGG
HapII 255 CCGG C^CGG
301 CCGG
HpaII 255 CCGG C^CGG
301 CCGG
MaeIII 369 GTCAC ^GT[ACGT]AC
376 GTGAC
MboII 174 TCTTC GAAGA(8/7)
251 TCTTC
MseI 59 TTAA T^TAA
383 TTAA
MspI 255 CCGG C^CGG
301 CCGG
NciI 255 CCGGG CC^[GC]GG
300 CCCGG
Tru9I 59 TTAA T^TAA
383 TTAA
Tsp45I 369 GTCAC ^GT[GC]AC
376 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI BanII BbvI BmyI BsaJI
BseRI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MboI MnlI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI TseI
Tsp509I