Restriction map for
pF1KB7777
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AviII AvrII BamHI BanI
BanII BbeI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaBI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HaeII HgaI HindIII
HpaI KasI KpnI KspI MamI
MluI MroI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 941 GACGTC GACGT^C
AcyI 941 GACGTC G[GA]^CG[CT]C
AflIII 1049 ACACGT A^C[GA][CT]GT
AhdI 1089 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1089 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 938 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 605 CTCGGG C^[CT]CG[GA]G
BbrPI 1050 CACGTG CAC^GTG
BbvCI 740 CCTCAGC CCTCAGC(-5/-2)
BbvI 1232 GCAGC GCAGC(8/12)
BpmI 1324 CTGGAG CTGGAG(16/14)
BsaAI 1050 CACGTG [CT]AC^GT[GA]
BsaHI 941 GACGTC G[GA]^CG[CT]C
BseRI 121 GAGGAG GAGGAG(10/8)
BsiEI 1254 CGGCCG CG[GA][CT]^CG
BsoBI 605 CTCGGG C^[CT]CG[GA]G
BspMI 395 GCAGGT ACCTGC(4/8)
BsrDI 1181 CATTGC GCAATG(2/0)
BstXI 446 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 101 CCATGG C^C[GA][CT]GG
EagI 1254 CGGCCG C^GGCCG
Eam1105I 1089 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1254 CGGCCG C^GGCCG
Eco52I 1254 CGGCCG C^GGCCG
EcoRV 976 GATATC GAT^ATC
EcoT14I 101 CCATGG C^C[AT][AT]GG
FauI 768 GCGGG CCCGC(4/6)
Hin1I 941 GACGTC G[GA]^CG[CT]C
HincII 1064 GTCGAC GT[CT]^[GA]AC
HindII 1064 GTCGAC GT[CT]^[GA]AC
MspA1I 1257 CCGCTG C[AC]G^C[GT]G
NcoI 101 CCATGG C^CATGG
PflFI 938 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 684 CCAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1050 CACGTG CAC^GTG
PmlI 1050 CACGTG CAC^GTG
SalI 1064 GTCGAC G^TCGAC
SapI 196 GAAGAGC GCTCTTC(1/4)
SfcI 552 CTGTAG C^T[GA][CT]AG
StyI 101 CCATGG C^C[AT][AT]GG
TseI 1232 GCAGC G^C[AT]GC
Tth111I 938 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 684 CCAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 554 GTAGAC GT^[AC][GT]AC
1064 GTCGAC
AccII 794 CGCG CG^CG
821 CGCG
AcsI 209 GAATTT [GA]^AATT[CT]
871 AAATTT
AfaI 727 GTAC GT^AC
933 GTAC
AlwI 24 GATCC GGATC(4/5)
1220 GGATC
AlwNI 233 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
1233 CAGCTACTG
ApoI 209 GAATTT [GA]^AATT[CT]
871 AAATTT
BalI 455 TGGCCA TGG^CCA
1286 TGGCCA
BsaJI 101 CCATGG C^C[ACGT][ACGT]GG
1104 CCAGGG
BsmAI 235 GAGAC GTCTC(1/5)
859 GTCTC
BsmFI 783 GGGAC GGGAC(10/14)
1205 GTCCC
BstNI 618 CCAGG CC^[AT]GG
1104 CCAGG
BstUI 794 CGCG CG^CG
821 CGCG
BstYI 23 AGATCC [GA]^GATC[CT]
1220 GGATCT
EcoRII 618 CCAGG ^CC[AT]GG
1104 CCAGG
MaeII 942 ACGT A^CGT
1051 ACGT
MfeI 911 CAATTG C^AATTG
1227 CAATTG
MflI 23 AGATCC [GA]^GATC[CT]
1220 GGATCT
MluNI 455 TGGCCA TGG^CCA
1286 TGGCCA
MscI 455 TGGCCA TGG^CCA
1286 TGGCCA
MseI 50 TTAA T^TAA
1313 TTAA
MunI 911 CAATTG C^AATTG
1227 CAATTG
MvaI 618 CCAGG CC^[AT]GG
1104 CCAGG
MvnI 794 CGCG CG^CG
821 CGCG
RsaI 727 GTAC GT^AC
933 GTAC
TfiI 577 GATTC G^A[AT]TC
1271 GAATC
Tru9I 50 TTAA T^TAA
1313 TTAA
Tsp45I 944 GTCAC ^GT[GC]AC
1250 GTGAC
XbaI 400 TCTAGA T^CTAGA
843 TCTAGA
XcmI 443 CCACCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1104 CCAGGGTTGCTGTGG
XhoII 23 AGATCC [GA]^GATC[CT]
1220 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AvaII BbsI BcnI
BfaI BpuAI BsiYI BslI BsrI
BssKI BstF5I Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MspI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI TaqI Tsp509I TspRI TthHB8I