##################################
# NO CUT ENZYMES (  190 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BsrI        
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FseI        HaeII       
HapII       HgaI        Hin1I       HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tru9I       Tth111I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI          217  GAATTT               [GA]^AATT[CT]
AfaI          281  GTAC                 GT^AC
AlwI           72  GGATC                GGATC(4/5)
ApoI          217  GAATTT               [GA]^AATT[CT]
Asp700I        35  GAAACCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         285  TGCGCA               TGC^GCA
BbvI           99  GCTGC                GCAGC(8/12)
BclI          363  TGATCA               T^GATCA
BsaJI          68  CCCTGG               C^C[ACGT][ACGT]GG
BseRI         480  CTCCTC               GAGGAG(10/8)
BsgI          126  CTGCAC               GTGCAG(16/14)
BspMI          27  ACCTGC               ACCTGC(4/8)
BsrDI         137  GCAATG               GCAATG(2/0)
BssSI         490  CACGAG               CACGAG(-5/-1)
BstF5I        345  CATCC                GGATG(2/0)
CfoI          286  GCGC                 GCG^C
Cfr13I        251  GGCCC                G^G[ACGT]CC
DraII         250  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      250  AGGCCCC              [GA]G^G[ACGT]CC[CT]
FbaI          363  TGATCA               T^GATCA
Fnu4HI         99  GCTGC                GC^[ACGT]GC
FokI          345  CATCC                GGATG(9/13)
FspI          285  TGCGCA               TGC^GCA
HaeIII        251  GGCC                 GG^CC
HhaI          286  GCGC                 GCG^C
HinP1I        286  GCGC                 G^CGC
HincII        499  GTTGAC               GT[CT]^[GA]AC
HindII        499  GTTGAC               GT[CT]^[GA]AC
ItaI           99  GCTGC                GC^[ACGT]GC
MslI          103  CATGAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
RsaI          281  GTAC                 GT^AC
SapI          223  GAAGAGC              GCTCTTC(1/4)
Sau96I        251  GGCCC                G^G[ACGT]CC
ScaI          280  AGTACT               AGT^ACT
SfaNI         262  GCATC                GCATC(5/9)
TseI           99  GCTGC                G^C[AT]GC
Tsp509I       218  AATT                 ^AATT
XcmI          396  CCATGTGAAGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI           35  GAAACCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AluI           98  AGCT                 AG^CT
              434  AGCT                
DpnII          73  GATC                 ^GATC
              364  GATC                
HphI           94  GGTGA                GGTGA(8/7)
              366  TCACC               
MboI           73  GATC                 ^GATC
              364  GATC                
NdeII          73  GATC                 ^GATC
              364  GATC                
NlaIV         251  GGCCCC               GG[ACGT]^[ACGT]CC
              412  GGCTCC              
Sau3AI         73  GATC                 ^GATC
              364  GATC                
SmlI          184  CTCAAG               C^T[CT][GA]AG
              352  CTTGAG              
TaqI           33  TCGA                 T^CGA
               75  TCGA                
TfiI          150  GAATC                G^A[AT]TC
              473  GAATC               
TthHB8I        33  TCGA                 T^CGA
               75  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

BssKI       BstAPI      BstNI       DdeI        EarI        
EcoRII      HinfI       Ksp632I     MaeIII      MboII       
MnlI        MvaI        MwoI        NlaIII      PleI        
ScrFI       Tsp45I      TspRI