Restriction map for
pF1KE0177
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MroI MseI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AluI 239 AGCT AG^CT
BalI 365 TGGCCA TGG^CCA
BbvCI 273 CCTCAGC CCTCAGC(-5/-2)
BcgI 278 GCATGCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 113 TGATCA T^GATCA
BcnI 176 CCCGG CC^[GC]GG
BfaI 539 CTAG C^TAG
BsgI 386 GTGCAG GTGCAG(16/14)
BsiYI 396 CCACCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 396 CCACCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 79 GGGAC GGGAC(10/14)
Bsp1407I 589 TGTACA T^GTACA
BsrGI 589 TGTACA T^GTACA
BstAPI 191 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 397 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 244 GACCTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 290 CCATGG C^C[GA][CT]GG
EaeI 365 TGGCCA [CT]^GGCC[GA]
EcoT14I 290 CCATGG C^C[AT][AT]GG
EcoT22I 649 ATGCAT ATGCA^T
FbaI 113 TGATCA T^GATCA
HapII 177 CCGG C^CGG
HincII 232 GTTGAC GT[CT]^[GA]AC
HindII 232 GTTGAC GT[CT]^[GA]AC
HpaII 177 CCGG C^CGG
HphI 165 GGTGA GGTGA(8/7)
MaeI 539 CTAG C^TAG
MboII 135 GAAGA GAAGA(8/7)
MluNI 365 TGGCCA TGG^CCA
MscI 365 TGGCCA TGG^CCA
MspA1I 238 CAGCTG C[AC]G^C[GT]G
MspI 177 CCGG C^CGG
NciI 176 CCCGG CC^[GC]GG
NcoI 290 CCATGG C^CATGG
NsiI 649 ATGCAT ATGCA^T
PflMI 396 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 81 GACTC GAGTC(4/5)
PpuMI 340 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 238 CAGCTG CAG^CTG
SexAI 398 ACCTGGT A^CC[AT]GGT
SphI 278 GCATGC GCATG^C
SspBI 589 TGTACA T^GTACA
StyI 290 CCATGG C^C[AT][AT]GG
TfiI 50 GAATC G^A[AT]TC
Van91I 396 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwNI 192 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
428 CAGGTACTG
AvaII 243 GGACC G^G[AT]CC
341 GGTCC
BanI 295 GGTGCC G^G[CT][GA]CC
348 GGTGCC
BpmI 256 CTCCAG CTGGAG(16/14)
96 CTGGAG
BsaJI 290 CCATGG C^C[ACGT][ACGT]GG
344 CCTCGG
BstNI 399 CCTGG CC^[AT]GG
423 CCTGG
BstXI 368 CCAAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
448 CCATCTATCTGG
DdeI 57 CTCAG C^T[ACGT]AG
274 CTCAG
DpnII 114 GATC ^GATC
285 GATC
DraII 340 AGGTCCT [GA]G^G[ACGT]CC[CT]
548 GGGGCCT
EcoO109I 340 AGGTCCT [GA]G^G[ACGT]CC[CT]
548 GGGGCCT
EcoRII 399 CCTGG ^CC[AT]GG
423 CCTGG
HinfI 50 GAATC G^A[ACGT]TC
81 GACTC
MaeIII 194 GTGAC ^GT[ACGT]AC
529 GTCAC
MboI 114 GATC ^GATC
285 GATC
MvaI 399 CCTGG CC^[AT]GG
423 CCTGG
NdeII 114 GATC ^GATC
285 GATC
NspI 278 GCATGC [GA]CATG^[CT]
592 ACATGC
Sau3AI 114 GATC ^GATC
285 GATC
StuI 66 AGGCCT AGG^CCT
156 AGGCCT
Tsp45I 194 GTGAC ^GT[GC]AC
529 GTCAC
Tsp509I 104 AATT ^AATT
702 AATT
XcmI 353 CCAACATCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
396 CCACCTGGTGGCTGG
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AfaI BbvI BmyI Bsp1286I BspMI
BsrI BssKI BstF5I Cac8I Cfr13I
Fnu4HI FokI HaeIII ItaI MnlI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI TseI TspRI