Restriction map for
pF1KE0114
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# NO CUT ENZYMES ( 136 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 157 GACGTC GACGT^C
AccI 46 GTAGAC GT^[AC][GT]AC
Alw44I 441 GTGCAC G^TGCAC
AlwI 462 GATCC GGATC(4/5)
AocI 799 CCTGAGG CC^T[ACGT]AGG
ApaLI 441 GTGCAC G^TGCAC
Asp700I 69 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 251 TGCGCA TGC^GCA
BalI 572 TGGCCA TGG^CCA
BanI 3 GGCGCC G^G[CT][GA]CC
BanII 238 GAGCTC G[GA]GC[CT]^C
BbeI 3 GGCGCC GGCGC^C
BbsI 160 GTCTTC GAAGAC(2/6)
BbvCI 703 GCTGAGG CCTCAGC(-5/-2)
BfaI 727 CTAG C^TAG
BpuAI 160 GTCTTC GAAGAC(2/6)
BseRI 225 CTCCTC GAGGAG(10/8)
BsmI 512 GAATGC GAATGC(1/-1)
BssSI 277 CACGAG CACGAG(-5/-1)
BstAPI 459 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 461 AGATCC [GA]^GATC[CT]
Bsu36I 799 CCTGAGG CC^T[ACGT]AGG
DsaI 575 CCACGG C^C[GA][CT]GG
EarI 507 GAAGAG CTCTTC(1/4)
Eco81I 799 CCTGAGG CC^T[ACGT]AGG
EcoT14I 875 CCAAGG C^C[AT][AT]GG
FauI 117 GCGGG CCCGC(4/6)
FspI 251 TGCGCA TGC^GCA
HgaI 753 GACGC GACGC(5/10)
HincII 760 GTCAAC GT[CT]^[GA]AC
HindII 760 GTCAAC GT[CT]^[GA]AC
HinfI 282 GAGTC G^A[ACGT]TC
KasI 3 GGCGCC G^GCGCC
Ksp632I 507 GAAGAG CTCTTC(1/4)
MaeI 727 CTAG C^TAG
MflI 461 AGATCC [GA]^GATC[CT]
MluNI 572 TGGCCA TGG^CCA
MscI 572 TGGCCA TGG^CCA
NarI 3 GGCGCC GG^CGCC
NspI 254 GCATGT [GA]CATG^[CT]
PleI 282 GAGTC GAGTC(4/5)
PshAI 753 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 826 CAGCTG CAG^CTG
SacI 238 GAGCTC GAGCT^C
SfoI 3 GGCGCC GGC^GCC
StyI 875 CCAAGG C^C[AT][AT]GG
TaqI 59 TCGA T^CGA
TthHB8I 59 TCGA T^CGA
XhoII 461 AGATCC [GA]^GATC[CT]
XmnI 69 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcyI 3 GGCGCC G[GA]^CG[CT]C
157 GACGTC
BpmI 241 CTCCAG CTGGAG(16/14)
436 CTGGAG
BsaHI 3 GGCGCC G[GA]^CG[CT]C
157 GACGTC
BsgI 418 CTGCAC GTGCAG(16/14)
457 GTGCAG
BsmAI 143 GAGAC GTCTC(1/5)
751 GAGAC
BsmBI 143 GAGACG CGTCTC(1/5)
751 GAGACG
DpnII 216 GATC ^GATC
462 GATC
EcoNI 522 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
711 CCTGCTGCAGG
Hin1I 3 GGCGCC G[GA]^CG[CT]C
157 GACGTC
MaeIII 340 GTGAC ^GT[ACGT]AC
805 GTGAC
MboI 216 GATC ^GATC
462 GATC
MslI 444 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
450 CATGACAGTG
NdeII 216 GATC ^GATC
462 GATC
PpuMI 518 AGGACCT [GA]G^G[AT]CC[CT]
844 AGGTCCC
PstI 592 CTGCAG CTGCA^G
715 CTGCAG
Sau3AI 216 GATC ^GATC
462 GATC
Tsp45I 340 GTGAC ^GT[GC]AC
805 GTGAC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeII MboII MnlI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PflMI RsaI Sau96I ScrFI
SfaNI SfcI TseI TspRI Van91I