Restriction map for
pF1KB6329
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AlwI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BciVI BclI BfrI BlnI
BlpI BpmI Bpu1102I BsaBI BseAI
BsgI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eco47III EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NcoI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 379 GTCTAC GT^[AC][GT]AC
AccII 333 CGCG CG^CG
AgeI 660 ACCGGT A^CCGGT
AhdI 70 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 304 GTGCAC G^TGCAC
AlwNI 482 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 241 CCTGAGG CC^T[ACGT]AGG
ApaLI 304 GTGCAC G^TGCAC
AspEI 70 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 44 CTCGGG C^[CT]CG[GA]G
BcgI 532 CGAGAGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 458 CTAG C^TAG
BglI 511 GCCTTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 269 AGATCT A^GATCT
BsaWI 660 ACCGGT [AT]^CCGG[AT]
BsiEI 11 CGGCCG CG[GA][CT]^CG
BsoBI 44 CTCGGG C^[CT]CG[GA]G
BssSI 669 CTCGTG CACGAG(-5/-1)
BstEII 67 GGTGACC G^GT[ACGT]ACC
BstPI 67 GGTGACC G^GT[ACGT]ACC
BstUI 333 CGCG CG^CG
BstYI 269 AGATCT [GA]^GATC[CT]
Bsu36I 241 CCTGAGG CC^T[ACGT]AGG
DsaI 769 CCACGG C^C[GA][CT]GG
EagI 11 CGGCCG C^GGCCG
Eam1105I 70 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 124 CTCTTC CTCTTC(1/4)
EclXI 11 CGGCCG C^GGCCG
Eco52I 11 CGGCCG C^GGCCG
Eco81I 241 CCTGAGG CC^T[ACGT]AGG
EcoO65I 67 GGTGACC G^GT[ACGT]ACC
EcoT14I 248 CCAAGG C^C[AT][AT]GG
Ksp632I 124 CTCTTC CTCTTC(1/4)
MaeI 458 CTAG C^TAG
MflI 269 AGATCT [GA]^GATC[CT]
MvnI 333 CGCG CG^CG
NheI 457 GCTAGC G^CTAGC
PinAI 660 ACCGGT A^CCGGT
PpuMI 569 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 219 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 415 CTGCAG CTGCA^G
SexAI 374 ACCAGGT A^CC[AT]GGT
SfaNI 110 GCATC GCATC(5/9)
SfcI 415 CTGCAG C^T[GA][CT]AG
SfiI 510 GGCCTTCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 248 CCAAGG C^C[AT][AT]GG
Tsp509I 619 AATT ^AATT
XhoII 269 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 177 GGCGCC G[GA]^CG[CT]C
202 GGCGCC
AflIII 383 ACGTGT A^C[GA][CT]GT
746 ACGTGT
ApaI 48 GGGCCC GGGCC^C
518 GGGCCC
AvaII 369 GGTCC G^G[AT]CC
570 GGACC
BanI 177 GGCGCC G^G[CT][GA]CC
202 GGCGCC
BbeI 177 GGCGCC GGCGC^C
202 GGCGCC
BbsI 544 GTCTTC GAAGAC(2/6)
637 GTCTTC
BpuAI 544 GTCTTC GAAGAC(2/6)
637 GTCTTC
BsaAI 382 TACGTG [CT]AC^GT[GA]
562 TACGTG
BsaHI 177 GGCGCC G[GA]^CG[CT]C
202 GGCGCC
BsaI 295 GAGACC GGTCTC(1/5)
590 GAGACC
BsmAI 295 GAGAC GTCTC(1/5)
590 GAGAC
BsrFI 578 GCCGGT [GA]^CCGG[CT]
660 ACCGGT
CfoI 178 GCGC GCG^C
203 GCGC
Cfr10I 578 GCCGGT [GA]^CCGG[CT]
660 ACCGGT
DpnII 27 GATC ^GATC
270 GATC
EaeI 11 CGGCCG [CT]^GGCC[GA]
772 CGGCCA
HaeII 177 GGCGCC [GA]GCGC^[CT]
202 GGCGCC
HgaI 393 GCGTC GACGC(5/10)
542 GCGTC
HhaI 178 GCGC GCG^C
203 GCGC
Hin1I 177 GGCGCC G[GA]^CG[CT]C
202 GGCGCC
HinP1I 178 GCGC G^CGC
203 GCGC
HinfI 259 GACTC G^A[ACGT]TC
535 GAGTC
HphI 67 GGTGA GGTGA(8/7)
622 TCACC
KasI 177 GGCGCC G^GCGCC
202 GGCGCC
MaeIII 68 GTGAC ^GT[ACGT]AC
689 GTGAC
MboI 27 GATC ^GATC
270 GATC
MslI 345 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
400 CATGTGGATG
MspA1I 52 CCGCTG C[AC]G^C[GT]G
171 CAGCGG
NarI 177 GGCGCC GG^CGCC
202 GGCGCC
NdeII 27 GATC ^GATC
270 GATC
NlaIII 400 CATG CATG^
684 CATG
PleI 259 GACTC GAGTC(4/5)
535 GAGTC
SacI 430 GAGCTC GAGCT^C
697 GAGCTC
Sau3AI 27 GATC ^GATC
270 GATC
SfoI 177 GGCGCC GGC^GCC
202 GGCGCC
Tsp45I 68 GTGAC ^GT[GC]AC
689 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AspHI BanII
BbvI BcnI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII ItaI MaeII MboII MnlI
MspI MvaI MwoI NciI NlaIV
RsaI Sau96I ScrFI TaqI TseI
TspRI TthHB8I