Restriction map for
pF1KB8598
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BspLU11I
BsrBI BsrDI BssHII Bst1107I BstAPI
BstBI BstZ17I Bsu36I CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV FseI
FspI HaeII KasI KpnI KspI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI Tth111I Van91I
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 1809 GTCGAC GT^[AC][GT]AC
AccII 1218 CGCG CG^CG
AcyI 1806 GGCGTC G[GA]^CG[CT]C
ApaI 663 GGGCCC GGGCC^C
AvaI 2231 CTCGAG C^[CT]CG[GA]G
AvrII 996 CCTAGG C^CTAGG
BalI 110 TGGCCA TGG^CCA
BclI 2243 TGATCA T^GATCA
BglI 1718 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 996 CCTAGG C^CTAGG
BlpI 1617 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1617 GCTGAGC GC^T[ACGT]AGC
BsaAI 622 TACGTG [CT]AC^GT[GA]
BsaBI 1821 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1806 GGCGTC G[GA]^CG[CT]C
BsgI 1864 GTGCAG GTGCAG(16/14)
BsoBI 2231 CTCGAG C^[CT]CG[GA]G
BspDI 377 ATCGAT AT^CGAT
BspMI 87 GCAGGT ACCTGC(4/8)
BsrFI 76 GCCGGC [GA]^CCGG[CT]
BssSI 185 CACGAG CACGAG(-5/-1)
BstEII 1221 GGTTACC G^GT[ACGT]ACC
BstPI 1221 GGTTACC G^GT[ACGT]ACC
BstUI 1218 CGCG CG^CG
BstXI 119 CCAGATATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1617 GCTGAGC GC^T[ACGT]AGC
Cfr10I 76 GCCGGC [GA]^CCGG[CT]
ClaI 377 ATCGAT AT^CGAT
DsaI 27 CCACGG C^C[GA][CT]GG
EarI 818 GAAGAG CTCTTC(1/4)
EcoO65I 1221 GGTTACC G^GT[ACGT]ACC
EcoRI 748 GAATTC G^AATTC
EcoT14I 996 CCTAGG C^C[AT][AT]GG
FauI 1487 GCGGG CCCGC(4/6)
FbaI 2243 TGATCA T^GATCA
Hin1I 1806 GGCGTC G[GA]^CG[CT]C
HindIII 1481 AAGCTT A^AGCTT
Ksp632I 818 GAAGAG CTCTTC(1/4)
MamI 1821 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 110 TGGCCA TGG^CCA
MscI 110 TGGCCA TGG^CCA
MvnI 1218 CGCG CG^CG
NaeI 76 GCCGGC GCC^GGC
NgoMIV 76 GCCGGC G^CCGGC
PaeR7I 2231 CTCGAG C^TCGAG
SalI 1809 GTCGAC G^TCGAC
ScaI 742 AGTACT AGT^ACT
SfcI 2313 CTACAG C^T[GA][CT]AG
SpeI 160 ACTAGT A^CTAGT
SspI 2279 AATATT AAT^ATT
StyI 996 CCTAGG C^C[AT][AT]GG
XbaI 979 TCTAGA T^CTAGA
XcmI 2181 CCAGCAGTTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2231 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 274 CTTAAG C^TTAAG
310 CTTAAG
AlwNI 767 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
1867 CAGCAGCTG
BcnI 11 CCCGG CC^[GC]GG
1625 CCGGG
BfrI 274 CTTAAG C^TTAAG
310 CTTAAG
BglII 56 AGATCT A^GATCT
1545 AGATCT
BsmI 1150 GCATTC GAATGC(1/-1)
1607 GCATTC
BspHI 837 TCATGA T^CATGA
2153 TCATGA
BstYI 56 AGATCT [GA]^GATC[CT]
1545 AGATCT
CfoI 1515 GCGC GCG^C
1909 GCGC
DraI 549 TTTAAA TTT^AAA
1077 TTTAAA
EcoT22I 650 ATGCAT ATGCA^T
2253 ATGCAT
HhaI 1515 GCGC GCG^C
1909 GCGC
HinP1I 1515 GCGC G^CGC
1909 GCGC
HpaI 672 GTTAAC GTT^AAC
2044 GTTAAC
MflI 56 AGATCT [GA]^GATC[CT]
1545 AGATCT
MspA1I 1870 CAGCTG C[AC]G^C[GT]G
2209 CAGCTG
NciI 11 CCCGG CC^[GC]GG
1625 CCGGG
NsiI 650 ATGCAT ATGCA^T
2253 ATGCAT
NspI 962 GCATGT [GA]CATG^[CT]
1517 GCATGT
PleI 1279 GACTC GAGTC(4/5)
2234 GAGTC
PpuMI 1627 GGGTCCT [GA]G^G[AT]CC[CT]
2198 AGGTCCT
PvuII 1870 CAGCTG CAG^CTG
2209 CAGCTG
RcaI 837 TCATGA T^CATGA
2153 TCATGA
XhoII 56 AGATCT [GA]^GATC[CT]
1545 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SmlI SspBI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I