Restriction map for
pF1KB0997
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspLU11I BspMI BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I MaeI MaeII MamI MfeI
MluI MseI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tru9I Tth111I TthHB8I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 230 TCCGGA T^CCGGA
AcsI 185 GAATTT [GA]^AATT[CT]
AfaI 120 GTAC GT^AC
AlwI 523 GGATC GGATC(4/5)
AocI 604 CCTGAGG CC^T[ACGT]AGG
ApoI 185 GAATTT [GA]^AATT[CT]
Asp700I 185 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 692 TGGCCA TGG^CCA
BanI 801 GGCACC G^G[CT][GA]CC
BglII 774 AGATCT A^GATCT
BsaWI 230 TCCGGA [AT]^CCGG[AT]
BseAI 230 TCCGGA T^CCGGA
BsmAI 227 GTCTC GTCTC(1/5)
BsmBI 226 CGTCTC CGTCTC(1/5)
BspEI 230 TCCGGA T^CCGGA
BspHI 218 TCATGA T^CATGA
BsrDI 137 GCAATG GCAATG(2/0)
BsrFI 780 GCCGGT [GA]^CCGG[CT]
BssSI 270 CACGAG CACGAG(-5/-1)
BstYI 774 AGATCT [GA]^GATC[CT]
Bsu36I 604 CCTGAGG CC^T[ACGT]AGG
CfoI 504 GCGC GCG^C
Cfr10I 780 GCCGGT [GA]^CCGG[CT]
EaeI 692 TGGCCA [CT]^GGCC[GA]
Eco81I 604 CCTGAGG CC^T[ACGT]AGG
EcoRV 454 GATATC GAT^ATC
EcoT14I 319 CCATGG C^C[AT][AT]GG
HaeII 503 AGCGCC [GA]GCGC^[CT]
HgaI 155 GCGTC GACGC(5/10)
HhaI 504 GCGC GCG^C
HinP1I 504 GCGC G^CGC
KspI 43 CCGCGG CCGC^GG
MflI 774 AGATCT [GA]^GATC[CT]
MluNI 692 TGGCCA TGG^CCA
MroI 230 TCCGGA T^CCGGA
MscI 692 TGGCCA TGG^CCA
NcoI 319 CCATGG C^CATGG
NspI 366 ACATGC [GA]CATG^[CT]
PvuII 596 CAGCTG CAG^CTG
RcaI 218 TCATGA T^CATGA
RsaI 120 GTAC GT^AC
SacI 544 GAGCTC GAGCT^C
SacII 43 CCGCGG CCGC^GG
SmaI 76 CCCGGG CCC^GGG
StuI 431 AGGCCT AGG^CCT
StyI 319 CCATGG C^C[AT][AT]GG
TfiI 222 GAATC G^A[AT]TC
XhoII 774 AGATCT [GA]^GATC[CT]
XmaCI 76 CCCGGG C^CCGGG
XmaI 76 CCCGGG C^CCGGG
XmnI 185 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 44 CGCG CG^CG
85 CGCG
AspHI 88 GTGCTC G[AT]GC[AT]^C
544 GAGCTC
BanII 48 GAGCCC G[GA]GC[CT]^C
544 GAGCTC
BcnI 76 CCCGG CC^[GC]GG
77 CCGGG
BpmI 770 CTCCAG CTGGAG(16/14)
291 CTGGAG
BseRI 741 GAGGAG GAGGAG(10/8)
744 GAGGAG
BsiHKAI 88 GTGCTC G[AT]GC[AT]^C
544 GAGCTC
BstF5I 29 CATCC GGATG(2/0)
705 CATCC
BstUI 44 CGCG CG^CG
85 CGCG
Cac8I 86 GCGTGC GC[ACGT]^[ACGT]GC
580 GCGGGC
DraII 79 GGGTCCC [GA]G^G[ACGT]CC[CT]
314 AGGACCC
DsaI 43 CCGCGG C^C[GA][CT]GG
319 CCATGG
EcoO109I 79 GGGTCCC [GA]G^G[ACGT]CC[CT]
314 AGGACCC
FokI 29 CATCC GGATG(9/13)
705 CATCC
HphI 710 TCACC GGTGA(8/7)
757 TCACC
MaeIII 157 GTCAC ^GT[ACGT]AC
756 GTCAC
MspA1I 43 CCGCGG C[AC]G^C[GT]G
596 CAGCTG
MvnI 44 CGCG CG^CG
85 CGCG
NciI 76 CCCGG CC^[GC]GG
77 CCGGG
PflMI 133 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
585 CCACAGTCTGG
PpuMI 79 GGGTCCC [GA]G^G[AT]CC[CT]
314 AGGACCC
SfaNI 474 GATGC GCATC(5/9)
445 GCATC
Tsp45I 157 GTCAC ^GT[GC]AC
756 GTCAC
Van91I 133 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
585 CCACAGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AvaI AvaII BbvI
BmyI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstNI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MboI MboII MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI TseI
Tsp509I TspRI