##################################
# NO CUT ENZYMES (  185 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AfaI        AflII       AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DpnII       DraI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HinfI       HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MamI        MboI        MfeI        MflI        MluNI       
MroI        MscI        MseI        MslI        MspA1I      
MunI        MvaI        NaeI        NarI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI          314  AAATTT               [GA]^AATT[CT]
AcyI           84  GACGCC               G[GA]^CG[CT]C
AflIII        156  ACGCGT               A^C[GA][CT]GT
AluI          137  AGCT                 AG^CT
Aor51HI       106  AGCGCT               AGC^GCT
ApaI           94  GGGCCC               GGGCC^C
ApoI          314  AAATTT               [GA]^AATT[CT]
AspHI         222  GTGCTC               G[AT]GC[AT]^C
AvaI           33  CCCGGG               C^[CT]CG[GA]G
BglI           87  GCCGCCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI          84  GACGCC               G[GA]^CG[CT]C
BsiHKAI       222  GTGCTC               G[AT]GC[AT]^C
BsiYI          27  CCGTGTCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           27  CCGTGTCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         118  GTCTC                GTCTC(1/5)
BsmFI          31  GTCCC                GGGAC(10/14)
BsoBI          33  CCCGGG               C^[CT]CG[GA]G
BspMI         198  GCAGGT               ACCTGC(4/8)
BssHII         56  GCGCGC               G^CGCGC
BstF5I        306  CATCC                GGATG(2/0)
DdeI          166  CTGAG                C^T[ACGT]AG
Eco47III      106  AGCGCT               AGC^GCT
EcoT14I       227  CCAAGG               C^C[AT][AT]GG
FokI          306  CATCC                GGATG(9/13)
HaeII         106  AGCGCT               [GA]GCGC^[CT]
HgaI           84  GACGC                GACGC(5/10)
Hin1I          84  GACGCC               G[GA]^CG[CT]C
MaeIII        160  GTTAC                ^GT[ACGT]AC
MboII         252  GAAGA                GAAGA(8/7)
MluI          156  ACGCGT               A^CGCGT
PpuMI          36  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          196  CTGCAG               CTGCA^G
SfcI          196  CTGCAG               C^T[GA][CT]AG
SmaI           33  CCCGGG               CCC^GGG
StyI          227  CCAAGG               C^C[AT][AT]GG
Tsp509I       315  AATT                 ^AATT
XcmI          227  CCAAGGTGGAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          33  CCCGGG               C^CCGGG
XmaI           33  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (    3 enzymes) #
###################################

BanII          45  GGGCTC               G[GA]GC[CT]^C
               94  GGGCCC              
FauI          208  CCCGC                CCCGC(4/6)
              216  CCCGC               
TspRI         220  CAGTG                CA[GC]TG[ACGT][ACGT]^
              324  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AccII       AciI        AvaII       BbvI        BcnI        
BmyI        BsaJI       Bsp1286I    BssKI       BstUI       
Cac8I       CfoI        Cfr13I      DraII       EcoO109I    
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MnlI        MspI        MvnI        
MwoI        NciI        NlaIV       Sau96I      ScrFI       
TseI