Restriction map for
pF1KE0365
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpmI BsaAI BsaBI BsaI
BseAI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BssHII BssSI Bst1107I BstBI
BstYI BstZ17I ClaI CpoI DraI
DraIII Eam1105I Eco47III EcoNI EcoRI
EcoRV FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MflI MluI MroI NdeI NheI
NotI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI SwaI TfiI
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Alw44I 787 GTGCAC G^TGCAC
ApaLI 787 GTGCAC G^TGCAC
Asp700I 171 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 821 GGCGCC GGCGC^C
BbsI 367 GTCTTC GAAGAC(2/6)
BcnI 161 CCCGG CC^[GC]GG
BfaI 836 CTAG C^TAG
BpuAI 367 GTCTTC GAAGAC(2/6)
BseRI 211 GAGGAG GAGGAG(10/8)
BsgI 1203 CTGCAC GTGCAG(16/14)
BsiEI 132 CGGCCG CG[GA][CT]^CG
BsmAI 432 GAGAC GTCTC(1/5)
BsmFI 1115 GGGAC GGGAC(10/14)
Bsp1407I 1022 TGTACA T^GTACA
BsrBI 817 GAGCGG CCGCTC(-3/-3)
BsrGI 1022 TGTACA T^GTACA
BstAPI 1065 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 878 CCAAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 132 CGGCCG C^GGCCG
EarI 1353 GAAGAG CTCTTC(1/4)
EclXI 132 CGGCCG C^GGCCG
Eco52I 132 CGGCCG C^GGCCG
HinfI 1235 GAGTC G^A[ACGT]TC
KasI 821 GGCGCC G^GCGCC
Ksp632I 1353 GAAGAG CTCTTC(1/4)
KspI 1197 CCGCGG CCGC^GG
MaeI 836 CTAG C^TAG
MaeII 1214 ACGT A^CGT
MfeI 636 CAATTG C^AATTG
MseI 412 TTAA T^TAA
MunI 636 CAATTG C^AATTG
NaeI 1061 GCCGGC GCC^GGC
NarI 821 GGCGCC GG^CGCC
NciI 161 CCCGG CC^[GC]GG
NcoI 545 CCATGG C^CATGG
NgoMIV 1061 GCCGGC G^CCGGC
NspI 522 GCATGC [GA]CATG^[CT]
PleI 1235 GAGTC GAGTC(4/5)
PpuMI 224 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 235 CAGCTG CAG^CTG
SacI 679 GAGCTC GAGCT^C
SacII 1197 CCGCGG CCGC^GG
SfoI 821 GGCGCC GGC^GCC
SmlI 219 CTTGAG C^T[CT][GA]AG
SphI 522 GCATGC GCATG^C
SspBI 1022 TGTACA T^GTACA
TaqI 1334 TCGA T^CGA
Tru9I 412 TTAA T^TAA
Tsp509I 637 AATT ^AATT
TthHB8I 1334 TCGA T^CGA
XmnI 171 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AocI 30 CCTCAGG CC^T[ACGT]AGG
1309 CCTGAGG
ApaI 276 GGGCCC GGGCC^C
1390 GGGCCC
AspI 313 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
361 GACATAGTC
BanI 821 GGCGCC G^G[CT][GA]CC
1037 GGCACC
BlpI 9 GCTGAGC GC^T[ACGT]AGC
909 GCTGAGC
Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC
909 GCTGAGC
BsaWI 143 TCCGGT [AT]^CCGG[AT]
719 TCCGGT
BsrFI 36 GCCGGT [GA]^CCGG[CT]
1061 GCCGGC
BstEII 184 GGTCACC G^GT[ACGT]ACC
1358 GGTCACC
BstPI 184 GGTCACC G^GT[ACGT]ACC
1358 GGTCACC
Bsu36I 30 CCTCAGG CC^T[ACGT]AGG
1309 CCTGAGG
CelII 9 GCTGAGC GC^T[ACGT]AGC
909 GCTGAGC
Cfr10I 36 GCCGGT [GA]^CCGG[CT]
1061 GCCGGC
DpnII 323 GATC ^GATC
672 GATC
Eco81I 30 CCTCAGG CC^T[ACGT]AGG
1309 CCTGAGG
EcoO65I 184 GGTCACC G^GT[ACGT]ACC
1358 GGTCACC
EcoT14I 545 CCATGG C^C[AT][AT]GG
866 CCAAGG
EcoT22I 520 ATGCAT ATGCA^T
615 ATGCAT
HgaI 453 GACGC GACGC(5/10)
1500 GACGC
MboI 323 GATC ^GATC
672 GATC
MslI 47 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
969 CACTTTCATG
MspA1I 235 CAGCTG C[AC]G^C[GT]G
1197 CCGCGG
NdeII 323 GATC ^GATC
672 GATC
NruI 1031 TCGCGA TCG^CGA
1418 TCGCGA
NsiI 520 ATGCAT ATGCA^T
615 ATGCAT
PflFI 313 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
361 GACATAGTC
PflMI 234 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1209 CCAGGACGTGG
PstI 607 CTGCAG CTGCA^G
777 CTGCAG
Sau3AI 323 GATC ^GATC
672 GATC
SfcI 607 CTGCAG C^T[GA][CT]AG
777 CTGCAG
StyI 545 CCATGG C^C[AT][AT]GG
866 CCAAGG
Tth111I 313 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
361 GACATAGTC
Van91I 234 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1209 CCAGGACGTGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaII BalI BanII
BbvI BmyI BsaHI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII DrdI DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeIII MboII MluNI MnlI MscI
MspI MvaI MvnI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI