Restriction map for
pF1KB6822
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BanII BbeI BbrPI
BbsI BbvCI BciVI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmFI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DsaI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp45I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AspHI 338 GTGCTC G[AT]GC[AT]^C
AvaI 35 CCCGAG C^[CT]CG[GA]G
AvaII 411 GGACC G^G[AT]CC
BamHI 204 GGATCC G^GATCC
BanI 321 GGTGCC G^G[CT][GA]CC
BcgI 219 CGACCGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 191 TGATCA T^GATCA
BcnI 70 CCGGG CC^[GC]GG
BglII 186 AGATCT A^GATCT
BsiEI 219 CGACCG CG[GA][CT]^CG
BsiHKAI 338 GTGCTC G[AT]GC[AT]^C
BsmI 312 GAATGC GAATGC(1/-1)
BsoBI 35 CCCGAG C^[CT]CG[GA]G
BsrDI 225 CATTGC GCAATG(2/0)
BsrI 414 CCAGT ACTGG(1/-1)
BssHII 79 GCGCGC G^CGCGC
BstAPI 489 GCAATTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 103 GACGGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 43 GAAGAG CTCTTC(1/4)
FbaI 191 TGATCA T^GATCA
HapII 70 CCGG C^CGG
HpaII 70 CCGG C^CGG
Ksp632I 43 GAAGAG CTCTTC(1/4)
MslI 534 CACGCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 22 CAGCGG C[AC]G^C[GT]G
MspI 70 CCGG C^CGG
NciI 70 CCGGG CC^[GC]GG
NspI 363 GCATGC [GA]CATG^[CT]
PflMI 414 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 436 GAGTC GAGTC(4/5)
PstI 376 CTGCAG CTGCA^G
SapI 43 GAAGAGC GCTCTTC(1/4)
SfaNI 238 GATGC GCATC(5/9)
SphI 363 GCATGC GCATG^C
TaqI 218 TCGA T^CGA
TfiI 165 GATTC G^A[AT]TC
TthHB8I 218 TCGA T^CGA
Van91I 414 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 440 CCAAGGCTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 80 CGCG CG^CG
82 CGCG
Aor51HI 53 AGCGCT AGC^GCT
380 AGCGCT
BfaI 262 CTAG C^TAG
523 CTAG
BmyI 322 GTGCCC G[AGT]GC[ACT]^C
338 GTGCTC
BsgI 118 GTGCAG GTGCAG(16/14)
496 GTGCAG
Bsp1286I 322 GTGCCC G[AGT]GC[ACT]^C
338 GTGCTC
BspMI 120 GCAGGT ACCTGC(4/8)
306 GCAGGT
BstF5I 258 CATCC GGATG(2/0)
504 GGATG
BstUI 80 CGCG CG^CG
82 CGCG
Eco47III 53 AGCGCT AGC^GCT
380 AGCGCT
EcoT14I 317 CCTTGG C^C[AT][AT]GG
440 CCAAGG
FokI 258 CATCC GGATG(9/13)
504 GGATG
HaeII 53 AGCGCT [GA]GCGC^[CT]
380 AGCGCT
HinfI 165 GATTC G^A[ACGT]TC
436 GAGTC
HphI 159 GGTGA GGTGA(8/7)
309 GGTGA
MaeI 262 CTAG C^TAG
523 CTAG
MvnI 80 CGCG CG^CG
82 CGCG
NlaIII 327 CATG CATG^
364 CATG
NlaIV 204 GGATCC GG[ACGT]^[ACGT]CC
321 GGTGCC
SfcI 28 CTACAG C^T[GA][CT]AG
376 CTGCAG
StyI 317 CCTTGG C^C[AT][AT]GG
440 CCAAGG
Tsp509I 254 AATT ^AATT
491 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BpmI BsaJI BseRI BsiYI BslI
BssKI BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoNI EcoRII
FauI Fnu4HI HaeIII HhaI HinP1I
ItaI MboI MboII MflI MnlI
MvaI MwoI NdeII RsaI Sau3AI
Sau96I ScrFI TseI TspRI XhoII