Restriction map for
pF1KB7639
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DpnII DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HindIII
HpaI KpnI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NdeII
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII Sau3AI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tth111I
XbaI XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 15 GTCGAC GT^[AC][GT]AC
AcsI 420 AAATTC [GA]^AATT[CT]
AfaI 615 GTAC GT^AC
AflIII 689 ACGTGT A^C[GA][CT]GT
ApoI 420 AAATTC [GA]^AATT[CT]
AvaI 179 CCCGGG C^[CT]CG[GA]G
AvaII 726 GGACC G^G[AT]CC
BfaI 196 CTAG C^TAG
BlpI 173 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 173 GCTTAGC GC^T[ACGT]AGC
BsmFI 725 GGGAC GGGAC(10/14)
BsoBI 179 CCCGGG C^[CT]CG[GA]G
BssHII 307 GCGCGC G^CGCGC
CelII 173 GCTTAGC GC^T[ACGT]AGC
DsaI 222 CCGCGG C^C[GA][CT]GG
EarI 363 CTCTTC CTCTTC(1/4)
EcoT14I 635 CCTTGG C^C[AT][AT]GG
FseI 484 GGCCGGCC GGCCGG^CC
HgaI 778 GACGC GACGC(5/10)
HincII 15 GTCGAC GT[CT]^[GA]AC
HindII 15 GTCGAC GT[CT]^[GA]AC
HinfI 477 GAGTC G^A[ACGT]TC
HphI 78 TCACC GGTGA(8/7)
Ksp632I 363 CTCTTC CTCTTC(1/4)
KspI 222 CCGCGG CCGC^GG
MaeI 196 CTAG C^TAG
MaeII 689 ACGT A^CGT
MboII 364 TCTTC GAAGA(8/7)
MslI 705 CACCGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 625 CATG CATG^
PflMI 666 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 477 GAGTC GAGTC(4/5)
PpuMI 725 GGGACCT [GA]G^G[AT]CC[CT]
PstI 814 CTGCAG CTGCA^G
PvuII 682 CAGCTG CAG^CTG
RsaI 615 GTAC GT^AC
SacII 222 CCGCGG CCGC^GG
SalI 15 GTCGAC G^TCGAC
SapI 362 GCTCTTC GCTCTTC(1/4)
ScaI 614 AGTACT AGT^ACT
SmaI 179 CCCGGG CCC^GGG
SmlI 720 CTCAAG C^T[CT][GA]AG
StuI 971 AGGCCT AGG^CCT
StyI 635 CCTTGG C^C[AT][AT]GG
Tsp509I 421 AATT ^AATT
Van91I 666 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 179 CCCGGG C^CCGGG
XmaI 179 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AlwNI 667 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
817 CAGGCCCTG
ApaI 182 GGGCCC GGGCC^C
521 GGGCCC
BseRI 37 GAGGAG GAGGAG(10/8)
943 GAGGAG
BsmAI 442 GAGAC GTCTC(1/5)
330 GTCTC
BspMI 245 ACCTGC ACCTGC(4/8)
290 ACCTGC
BsrI 325 CCAGT ACTGG(1/-1)
491 CCAGT
BstAPI 261 GCAACTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
816 GCAGGCCCTGC
EaeI 322 CGGCCA [CT]^GGCC[GA]
918 TGGCCG
SfaNI 66 GATGC GCATC(5/9)
652 GCATC
SfcI 342 CTATAG C^T[GA][CT]AG
814 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
BanI BanII BbeI BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BsrFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI KasI MaeIII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NgoMIV
NlaIV SacI Sau96I ScrFI SfoI
TaqI TseI Tsp45I TspRI TthHB8I