Restriction map for
pF1KE0562
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvrII BamHI
BbrPI BbvCI BciVI BcnI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI HapII HgaI HincII HindII
HpaI HpaII KpnI Ksp632I KspI
MaeII MamI MfeI MluI MroI
MseI MspA1I MspI MunI MvnI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tru9I Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 100 GTCTAC GT^[AC][GT]AC
AcyI 331 GGCGCC G[GA]^CG[CT]C
Asp700I 372 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 611 TGCGCA TGC^GCA
BanI 331 GGCGCC G^G[CT][GA]CC
BbeI 331 GGCGCC GGCGC^C
BcgI 599 GCAAGGGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 678 TGATCA T^GATCA
BfaI 20 CTAG C^TAG
BlpI 498 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 498 GCTGAGC GC^T[ACGT]AGC
BsaHI 331 GGCGCC G[GA]^CG[CT]C
BsaI 961 GAGACC GGTCTC(1/5)
BseRI 392 CTCCTC GAGGAG(10/8)
BsmAI 961 GAGAC GTCTC(1/5)
Bsp1407I 710 TGTACA T^GTACA
BspMI 104 ACCTGC ACCTGC(4/8)
BsrGI 710 TGTACA T^GTACA
BssSI 520 CTCGTG CACGAG(-5/-1)
BstXI 504 CCATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 498 GCTGAGC GC^T[ACGT]AGC
FbaI 678 TGATCA T^GATCA
FspI 611 TGCGCA TGC^GCA
HaeII 331 GGCGCC [GA]GCGC^[CT]
Hin1I 331 GGCGCC G[GA]^CG[CT]C
HindIII 774 AAGCTT A^AGCTT
HinfI 33 GAGTC G^A[ACGT]TC
KasI 331 GGCGCC G^GCGCC
MaeI 20 CTAG C^TAG
NarI 331 GGCGCC GG^CGCC
PleI 33 GAGTC GAGTC(4/5)
PpuMI 269 AGGACCT [GA]G^G[AT]CC[CT]
PstI 46 CTGCAG CTGCA^G
SfcI 46 CTGCAG C^T[GA][CT]AG
SfoI 331 GGCGCC GGC^GCC
SspBI 710 TGTACA T^GTACA
StuI 814 AGGCCT AGG^CCT
Tsp509I 858 AATT ^AATT
XmnI 372 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 127 CCGC CCGC(-3/-1)
732 CCGC
AfaI 444 GTAC GT^AC
711 GTAC
AlwNI 31 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
682 CAGGTGCTG
AspHI 283 GAGCAC G[AT]GC[AT]^C
465 GTGCTC
BalI 401 TGGCCA TGG^CCA
452 TGGCCA
BanII 632 GGGCTC G[GA]GC[CT]^C
796 GGGCTC
BsgI 348 CTGCAC GTGCAG(16/14)
916 CTGCAC
BsiHKAI 283 GAGCAC G[AT]GC[AT]^C
465 GTGCTC
BsmFI 236 GGGAC GGGAC(10/14)
643 GTCCC
BstAPI 341 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
558 GCAAAGTGTGC
BstYI 80 GGATCT [GA]^GATC[CT]
425 AGATCC
DraII 269 AGGACCT [GA]G^G[ACGT]CC[CT]
551 AGGCCCT
DsaI 312 CCATGG C^C[GA][CT]GG
504 CCATGG
EcoNI 200 CCTGGGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
431 CCTTTGTTAGG
EcoO109I 269 AGGACCT [GA]G^G[ACGT]CC[CT]
551 AGGCCCT
EcoT14I 312 CCATGG C^C[AT][AT]GG
504 CCATGG
MaeIII 23 GTCAC ^GT[ACGT]AC
823 GTCAC
MflI 80 GGATCT [GA]^GATC[CT]
425 AGATCC
MluNI 401 TGGCCA TGG^CCA
452 TGGCCA
MscI 401 TGGCCA TGG^CCA
452 TGGCCA
NcoI 312 CCATGG C^CATGG
504 CCATGG
PflMI 71 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
873 CCAGGAGATGG
RsaI 444 GTAC GT^AC
711 GTAC
StyI 312 CCATGG C^C[AT][AT]GG
504 CCATGG
TaqI 370 TCGA T^CGA
929 TCGA
Tsp45I 23 GTCAC ^GT[GC]AC
823 GTCAC
TthHB8I 370 TCGA T^CGA
929 TCGA
Van91I 71 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
873 CCAGGAGATGG
XhoII 80 GGATCT [GA]^GATC[CT]
425 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AluI AlwI AvaII BbsI BbvI
BmyI BpmI BpuAI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HhaI HinP1I HphI ItaI
MboI MboII MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TseI TspRI