################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI Aor51HI ApaI AscI Asp700I Asp718I AvaI AviII AvrII BamHI BbrPI BcgI BlnI BsaAI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII BstEII BstPI Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HpaI KpnI KspI MfeI MluI MroI MunI NaeI NgoMIV NotI PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PstI PvuI RsrII SacII SalI SbfI SexAI SfiI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcyI 3623 GGCGCC G[GA]^CG[CT]C AhdI 3101 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2709 GTGCAC G^TGCAC ApaLI 2709 GTGCAC G^TGCAC AspEI 3101 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 5413 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 3623 GGCGCC GGCGC^C BbvCI 6115 CCTCAGC CCTCAGC(-5/-2) BcnI 3822 CCCGG CC^[GC]GG BglI 6460 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 4823 GCTTAGC GC^T[ACGT]AGC Bpu1102I 4823 GCTTAGC GC^T[ACGT]AGC BsaHI 3623 GGCGCC G[GA]^CG[CT]C BsaWI 3794 TCCGGT [AT]^CCGG[AT] BsgI 5365 GTGCAG GTGCAG(16/14) BssSI 2339 CACGAG CACGAG(-5/-1) BstXI 5450 CCAGTTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 4823 GCTTAGC GC^T[ACGT]AGC DraIII 3156 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 3101 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 640 GAATTC G^AATTC HaeII 3623 GGCGCC [GA]GCGC^[CT] Hin1I 3623 GGCGCC G[GA]^CG[CT]C KasI 3623 GGCGCC G^GCGCC NarI 3623 GGCGCC GG^CGCC NciI 3822 CCCGG CC^[GC]GG NdeI 4046 CATATG CA^TATG NheI 4272 GCTAGC G^CTAGC NruI 1353 TCGCGA TCG^CGA PflFI 5413 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3020 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 3227 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 438 AGTACT AGT^ACT SfoI 3623 GGCGCC GGC^GCC Tth111I 5413 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3020 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### AflII 1470 CTTAAG C^TTAAG 3076 CTTAAG AocI 1074 CCTCAGG CC^T[ACGT]AGG 5869 CCTGAGG AseI 418 ATTAAT AT^TAAT 6337 ATTAAT AsnI 418 ATTAAT AT^TAAT 6337 ATTAAT BalI 3808 TGGCCA TGG^CCA 5186 TGGCCA BfrI 1470 CTTAAG C^TTAAG 3076 CTTAAG BglII 480 AGATCT A^GATCT 2001 AGATCT BsaBI 2020 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4000 GATAAGAATC BsaI 6268 GAGACC GGTCTC(1/5) 1180 GGTCTC BseRI 1296 GAGGAG GAGGAG(10/8) 5942 GAGGAG Bsp1407I 3389 TGTACA T^GTACA 5745 TGTACA BspMI 6680 ACCTGC ACCTGC(4/8) 6652 GCAGGT BsrGI 3389 TGTACA T^GTACA 5745 TGTACA Bst1107I 509 GTATAC GTA^TAC 3497 GTATAC BstAPI 5125 GCATTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5554 GCAAAGGGTGC BstBI 2234 TTCGAA TT^CGAA 5675 TTCGAA BstZ17I 509 GTATAC GTA^TAC 3497 GTATAC Bsu36I 1074 CCTCAGG CC^T[ACGT]AGG 5869 CCTGAGG CfoI 3624 GCGC GCG^C 6107 GCGC DsaI 1721 CCATGG C^C[GA][CT]GG 6254 CCATGG EaeI 3808 TGGCCA [CT]^GGCC[GA] 5186 TGGCCA Eco81I 1074 CCTCAGG CC^T[ACGT]AGG 5869 CCTGAGG EcoNI 2460 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4611 CCTATTTCAGG HgaI 547 GCGTC GACGC(5/10) 1359 GCGTC HhaI 3624 GCGC GCG^C 6107 GCGC HinP1I 3624 GCGC G^CGC 6107 GCGC HincII 4288 GTCAAC GT[CT]^[GA]AC 5419 GTCAAC HindII 4288 GTCAAC GT[CT]^[GA]AC 5419 GTCAAC MaeII 792 ACGT A^CGT 5683 ACGT MamI 2020 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4000 GATAAGAATC MluNI 3808 TGGCCA TGG^CCA 5186 TGGCCA MscI 3808 TGGCCA TGG^CCA 5186 TGGCCA MspA1I 1925 CAGCTG C[AC]G^C[GT]G 6118 CAGCTG NcoI 1721 CCATGG C^CATGG 6254 CCATGG NspV 2234 TTCGAA TT^CGAA 5675 TTCGAA PshBI 418 ATTAAT AT^TAAT 6337 ATTAAT PvuII 1925 CAGCTG CAG^CTG 6118 CAGCTG SacI 4315 GAGCTC GAGCT^C 6034 GAGCTC SapI 4312 GAAGAGC GCTCTTC(1/4) 5461 GCTCTTC SfuI 2234 TTCGAA TT^CGAA 5675 TTCGAA SphI 1040 GCATGC GCATG^C 4832 GCATGC SspBI 3389 TGTACA T^GTACA 5745 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BciVI BclI BfaI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII