##################################
# NO CUT ENZYMES (   31 enzymes) #
##################################

AclI        AgeI        AscI        AseI        AsnI        
AvrII       BlnI        BsiWI       BssHII      Bst1107I    
BstZ17I     CpoI        FseI        KspI        MluI        
NotI        PacI        PinAI       PmeI        PshBI       
Psp1406I    RsrII       SacII       SbfI        SfiI        
SgrAI       SnaBI       SpeI        Sse8387I    SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   14 enzymes) #
###################################

AatII         259  GACGTC               GACGT^C
Acc65I       3008  GGTACC               G^GTACC
AflII        2197  CTTAAG               C^TTAAG
Asp718I      3008  GGTACC               G^GTACC
BfrI         2197  CTTAAG               C^TTAAG
BstBI       13870  TTCGAA               TT^CGAA
EcoT22I      1204  ATGCAT               ATGCA^T
KpnI         3008  GGTACC               GGTAC^C
NheI         9480  GCTAGC               G^CTAGC
NruI         1803  TCGCGA               TCG^CGA
NsiI         1204  ATGCAT               ATGCA^T
NspV        13870  TTCGAA               TT^CGAA
PvuI        11994  CGATCG               CGAT^CG
SfuI        13870  TTCGAA               TT^CGAA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

Alw44I      10874  GTGCAC               G^TGCAC
            13929  GTGCAC              
AocI         1449  CCTGAGG              CC^T[ACGT]AGG
            13549  CCTGAGG             
ApaI         6670  GGGCCC               GGGCC^C
             8217  GGGCCC              
ApaLI       10874  GTGCAC               G^TGCAC
            13929  GTGCAC              
BciVI        8358  GGATAC               GTATCC(6/5)
            10755  GTATCC              
BspDI        4027  ATCGAT               AT^CGAT
             7603  ATCGAT              
BspHI        2877  TCATGA               T^CATGA
             4247  TCATGA              
BssSI        9139  CACGAG               CACGAG(-5/-1)
             9562  CACGAG              
Bsu36I       1449  CCTGAGG              CC^T[ACGT]AGG
            13549  CCTGAGG             
ClaI         4027  ATCGAT               AT^CGAT
             7603  ATCGAT              
DraI         5056  TTTAAA               TTT^AAA
             6217  TTTAAA              
Eco81I       1449  CCTGAGG              CC^T[ACGT]AGG
            13549  CCTGAGG             
HpaI          745  GTTAAC               GTT^AAC
             2209  GTTAAC              
NdeI         2492  CATATG               CA^TATG
             6575  CATATG              
PaeR7I       6730  CTCGAG               C^TCGAG
            11615  CTCGAG              
RcaI         2877  TCATGA               T^CATGA
             4247  TCATGA              
SalI         9634  GTCGAC               G^TCGAC
            12862  GTCGAC              
XhoI         6730  CTCGAG               C^TCGAG
            11615  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  195 enzymes) #
#########################################

AccI        AccII       AccIII      AciI        AcsI        
AcyI        AfaI        AflIII      AhdI        AluI        
AlwI        AlwNI       Aor51HI     ApoI        Asp700I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BclI        BcnI        BfaI        BglI        
BglII       BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssKI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     FauI        FbaI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MfeI        MflI        MluNI       MnlI        MroI        
MscI        MseI        MslI        MspA1I      MspI        
MunI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        PmaCI       PmlI        PpuMI       PshAI       
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScaI        ScrFI       SexAI       
SfaNI       SfcI        SfoI        SmaI        SmlI        
SphI        SspBI       SspI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI        XmnI