Restriction map for
pF1KB7325
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AflII AhdI AscI
Asp700I Asp718I AspEI AviII AvrII
BbeI BciVI BfrI BlnI BsaBI
BsiWI BsmBI BsmI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
DraIII Eam1105I EcoO65I EcoT22I FseI
FspI HpaI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PmeI PvuI SacI
SacII SbfI SexAI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 45 TCCGGA T^CCGGA
AclI 596 AACGTT AA^CGTT
AflIII 3420 ACGTGT A^C[GA][CT]GT
AgeI 141 ACCGGT A^CCGGT
AocI 728 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2147 AGCGCT AGC^GCT
AseI 3828 ATTAAT AT^TAAT
AsnI 3828 ATTAAT AT^TAAT
AspI 2461 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1309 GGCACC G^G[CT][GA]CC
BbrPI 385 CACGTG CAC^GTG
BsaAI 385 CACGTG [CT]AC^GT[GA]
BsaI 1152 GGTCTC GGTCTC(1/5)
BseAI 45 TCCGGA T^CCGGA
Bsp1407I 4910 TGTACA T^GTACA
BspEI 45 TCCGGA T^CCGGA
BsrGI 4910 TGTACA T^GTACA
BstAPI 3033 GCATGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1115 CCATCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 728 CCTCAGG CC^T[ACGT]AGG
CpoI 138 CGGACCG CG^G[AT]CCG
DrdI 1911 GACACTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2147 AGCGCT AGC^GCT
Eco81I 728 CCTCAGG CC^T[ACGT]AGG
EcoRI 1036 GAATTC G^AATTC
EcoRV 4140 GATATC GAT^ATC
HindIII 4505 AAGCTT A^AGCTT
MroI 45 TCCGGA T^CCGGA
NheI 4608 GCTAGC G^CTAGC
PflFI 2461 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 141 ACCGGT A^CCGGT
PmaCI 385 CACGTG CAC^GTG
PmlI 385 CACGTG CAC^GTG
PshAI 4473 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3828 ATTAAT AT^TAAT
Psp1406I 596 AACGTT AA^CGTT
RsrII 138 CGGACCG CG^G[AT]CCG
SalI 3764 GTCGAC G^TCGAC
SfiI 84 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2910 GCATGC GCATG^C
SspBI 4910 TGTACA T^GTACA
SspI 4749 AATATT AAT^ATT
Tth111I 2461 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3458 CCAAAGAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 35 enzymes) #
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Alw44I 838 GTGCAC G^TGCAC
2383 GTGCAC
ApaI 17 GGGCCC GGGCC^C
2017 GGGCCC
ApaLI 838 GTGCAC G^TGCAC
2383 GTGCAC
BamHI 3356 GGATCC G^GATCC
3641 GGATCC
BbvCI 1470 CCTCAGC CCTCAGC(-5/-2)
2112 CCTCAGC
BcgI 4512 CGATAATCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1197 GCATCAGCTTCG
BclI 825 TGATCA T^GATCA
3935 TGATCA
BglI 85 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1175 GCCACTCAGGC
BglII 3098 AGATCT A^GATCT
3804 AGATCT
BlpI 586 GCTAAGC GC^T[ACGT]AGC
1219 GCTCAGC
Bpu1102I 586 GCTAAGC GC^T[ACGT]AGC
1219 GCTCAGC
BsaWI 45 TCCGGA [AT]^CCGG[AT]
141 ACCGGT
BspDI 3127 ATCGAT AT^CGAT
3446 ATCGAT
BspHI 435 TCATGA T^CATGA
4335 TCATGA
BsrBI 1969 GAGCGG CCGCTC(-3/-3)
1978 GAGCGG
BsrDI 1087 GCAATG GCAATG(2/0)
2330 GCAATG
BssSI 73 CACGAG CACGAG(-5/-1)
1975 CACGAG
CelII 586 GCTAAGC GC^T[ACGT]AGC
1219 GCTCAGC
ClaI 3127 ATCGAT AT^CGAT
3446 ATCGAT
DraI 3223 TTTAAA TTT^AAA
3399 TTTAAA
FbaI 825 TGATCA T^GATCA
3935 TGATCA
HincII 2554 GTCAAC GT[CT]^[GA]AC
3764 GTCGAC
HindII 2554 GTCAAC GT[CT]^[GA]AC
3764 GTCGAC
NcoI 2819 CCATGG C^CATGG
4575 CCATGG
NspI 2910 GCATGC [GA]CATG^[CT]
3195 GCATGT
PflMI 2763 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3987 CCAGTTTGTGG
PstI 4326 CTGCAG CTGCA^G
4459 CTGCAG
RcaI 435 TCATGA T^CATGA
4335 TCATGA
SapI 502 GAAGAGC GCTCTTC(1/4)
2418 GAAGAGC
ScaI 2408 AGTACT AGT^ACT
4322 AGTACT
SmaI 22 CCCGGG CCC^GGG
2181 CCCGGG
SmlI 182 CTCAAG C^T[CT][GA]AG
2986 CTCAAG
Van91I 2763 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3987 CCAGTTTGTGG
XmaCI 22 CCCGGG C^CCGGG
2181 CCCGGG
XmaI 22 CCCGGG C^CCGGG
2181 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII