Restriction map for
pF1KB7851
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AclI AflII AflIII AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BalI BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MluNI MscI MunI
NarI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 935 GGTACC G^GTACC
AccI 961 GTCTAC GT^[AC][GT]AC
AccIII 633 TCCGGA T^CCGGA
AcsI 160 GAATTC [GA]^AATT[CT]
AgeI 1074 ACCGGT A^CCGGT
AlwNI 558 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 919 CCTGAGG CC^T[ACGT]AGG
ApoI 160 GAATTC [GA]^AATT[CT]
Asp700I 297 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 935 GGTACC G^GTACC
BglI 536 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1066 GAGACC GGTCTC(1/5)
BseAI 633 TCCGGA T^CCGGA
BsmI 275 GCATTC GAATGC(1/-1)
BspEI 633 TCCGGA T^CCGGA
BsrDI 135 CATTGC GCAATG(2/0)
Bsu36I 919 CCTGAGG CC^T[ACGT]AGG
DrdI 682 GACCCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 758 TGGCCG [CT]^GGCC[GA]
Eco81I 919 CCTGAGG CC^T[ACGT]AGG
EcoRI 160 GAATTC G^AATTC
KpnI 935 GGTACC GGTAC^C
MaeII 577 ACGT A^CGT
MroI 633 TCCGGA T^CCGGA
NaeI 1157 GCCGGC GCC^GGC
NcoI 1055 CCATGG C^CATGG
NgoMIV 1157 GCCGGC G^CCGGC
NheI 1245 GCTAGC G^CTAGC
PinAI 1074 ACCGGT A^CCGGT
PpuMI 470 AGGACCC [GA]G^G[AT]CC[CT]
PstI 940 CTGCAG CTGCA^G
SacI 1590 GAGCTC GAGCT^C
SapI 494 GCTCTTC GCTCTTC(1/4)
SbfI 939 CCTGCAGG CCTGCA^GG
SfaNI 1420 GCATC GCATC(5/9)
SmaI 1414 CCCGGG CCC^GGG
Sse8387I 939 CCTGCAGG CCTGCA^GG
TaqI 1481 TCGA T^CGA
TthHB8I 1481 TCGA T^CGA
XmaCI 1414 CCCGGG C^CCGGG
XmaI 1414 CCCGGG C^CCGGG
XmnI 297 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 613 CGCG CG^CG
985 CGCG
AspHI 1590 GAGCTC G[AT]GC[AT]^C
1630 GAGCAC
BamHI 606 GGATCC G^GATCC
769 GGATCC
BfaI 640 CTAG C^TAG
1246 CTAG
BlpI 240 GCTGAGC GC^T[ACGT]AGC
846 GCTGAGC
Bpu1102I 240 GCTGAGC GC^T[ACGT]AGC
846 GCTGAGC
BsaWI 633 TCCGGA [AT]^CCGG[AT]
1074 ACCGGT
BsiHKAI 1590 GAGCTC G[AT]GC[AT]^C
1630 GAGCAC
BsmAI 1066 GAGAC GTCTC(1/5)
1637 GAGAC
BspMI 938 ACCTGC ACCTGC(4/8)
1380 ACCTGC
BsrBI 477 CCGCTC CCGCTC(-3/-3)
1203 GAGCGG
BstUI 613 CGCG CG^CG
985 CGCG
BstXI 678 CCATGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
815 CCACCCACCTGG
CelII 240 GCTGAGC GC^T[ACGT]AGC
846 GCTGAGC
DsaI 1055 CCATGG C^C[GA][CT]GG
1610 CCACGG
MaeI 640 CTAG C^TAG
1246 CTAG
MaeIII 560 GTGAC ^GT[ACGT]AC
1318 GTGAC
MseI 1130 TTAA T^TAA
1451 TTAA
MslI 354 CATTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1421 CATCCCAGTG
MvnI 613 CGCG CG^CG
985 CGCG
NspI 1430 GCATGC [GA]CATG^[CT]
1605 GCATGC
PvuII 82 CAGCTG CAG^CTG
1169 CAGCTG
SfcI 564 CTGTAG C^T[GA][CT]AG
940 CTGCAG
SmlI 69 CTCAAG C^T[CT][GA]AG
1438 CTCAAG
SphI 1430 GCATGC GCATG^C
1605 GCATGC
Tru9I 1130 TTAA T^TAA
1451 TTAA
Tsp45I 560 GTGAC ^GT[GC]AC
1318 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
ApaI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
StyI TseI Tsp509I TspRI XhoII