Restriction map for
pF1KB8348
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# NO CUT ENZYMES ( 103 enzymes) #
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Acc65I AccI AccIII AclI AflII
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BbrPI
BciVI BclI BfrI BglI BglII
BlnI BsaAI BsaI BseAI BsiEI
BsiWI BsmBI BsoBI BspEI BspLU11I
BspMI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoRV
FbaI FseI FspI HindIII HpaI
KpnI KspI MfeI MroI MunI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI SwaI XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AatII 460 GACGTC GACGT^C
AcsI 1359 GAATTC [GA]^AATT[CT]
AflIII 147 ACGCGT A^C[GA][CT]GT
AgeI 781 ACCGGT A^CCGGT
AlwNI 421 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1151 AGCGCT AGC^GCT
ApoI 1359 GAATTC [GA]^AATT[CT]
AspHI 955 GAGCTC G[AT]GC[AT]^C
AspI 99 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1026 GGATCC G^GATCC
BbeI 1293 GGCGCC GGCGC^C
BbsI 850 GAAGAC GAAGAC(2/6)
BbvCI 154 CCTCAGC CCTCAGC(-5/-2)
BcgI 368 GCAGAGTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1092 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1092 GCTCAGC GC^T[ACGT]AGC
BpuAI 850 GAAGAC GAAGAC(2/6)
BsaBI 871 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 781 ACCGGT [AT]^CCGG[AT]
BsgI 677 GTGCAG GTGCAG(16/14)
BsiHKAI 955 GAGCTC G[AT]GC[AT]^C
BsmFI 151 GTCCC GGGAC(10/14)
BsmI 250 GAATGC GAATGC(1/-1)
Bsp1407I 1223 TGTACA T^GTACA
BspDI 487 ATCGAT AT^CGAT
BspHI 1130 TCATGA T^CATGA
BsrBI 1057 GAGCGG CCGCTC(-3/-3)
BsrFI 781 ACCGGT [GA]^CCGG[CT]
BsrGI 1223 TGTACA T^GTACA
BstEII 189 GGTCACC G^GT[ACGT]ACC
BstPI 189 GGTCACC G^GT[ACGT]ACC
CelII 1092 GCTCAGC GC^T[ACGT]AGC
Cfr10I 781 ACCGGT [GA]^CCGG[CT]
ClaI 487 ATCGAT AT^CGAT
DraII 1190 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 1151 AGCGCT AGC^GCT
EcoO109I 1190 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 189 GGTCACC G^GT[ACGT]ACC
EcoRI 1359 GAATTC G^AATTC
EcoT22I 655 ATGCAT ATGCA^T
FauI 269 GCGGG CCCGC(4/6)
HincII 463 GTCAAC GT[CT]^[GA]AC
HindII 463 GTCAAC GT[CT]^[GA]AC
KasI 1293 GGCGCC G^GCGCC
MaeII 461 ACGT A^CGT
MamI 871 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 147 ACGCGT A^CGCGT
MslI 195 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 981 CAGCTG C[AC]G^C[GT]G
NarI 1293 GGCGCC GG^CGCC
NcoI 1143 CCATGG C^CATGG
NsiI 655 ATGCAT ATGCA^T
PflFI 99 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 916 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 781 ACCGGT A^CCGGT
PvuII 981 CAGCTG CAG^CTG
RcaI 1130 TCATGA T^CATGA
SacI 955 GAGCTC GAGCT^C
SapI 1062 GAAGAGC GCTCTTC(1/4)
SfaNI 986 GCATC GCATC(5/9)
SfoI 1293 GGCGCC GGC^GCC
SmlI 231 CTCAAG C^T[CT][GA]AG
SphI 657 GCATGC GCATG^C
SspBI 1223 TGTACA T^GTACA
TfiI 882 GATTC G^A[AT]TC
Tth111I 99 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 916 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcyI 460 GACGTC G[GA]^CG[CT]C
1293 GGCGCC
BanI 1000 GGCACC G^G[CT][GA]CC
1293 GGCGCC
BcnI 1043 CCCGG CC^[GC]GG
1209 CCCGG
BsaHI 460 GACGTC G[GA]^CG[CT]C
1293 GGCGCC
BsmAI 589 GAGAC GTCTC(1/5)
171 GTCTC
BstF5I 558 CATCC GGATG(2/0)
302 GGATG
BstXI 194 CCATCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
219 CCAGGTGAATGG
EarI 596 CTCTTC CTCTTC(1/4)
1062 GAAGAG
FokI 558 CATCC GGATG(9/13)
302 GGATG
Hin1I 460 GACGTC G[GA]^CG[CT]C
1293 GGCGCC
Ksp632I 596 CTCTTC CTCTTC(1/4)
1062 GAAGAG
MseI 14 TTAA T^TAA
652 TTAA
MwoI 159 GCAGGATTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
417 GCGGCAGTGGC
NciI 1043 CCCGG CC^[GC]GG
1209 CCCGG
NdeI 752 CATATG CA^TATG
1408 CATATG
NspI 335 ACATGC [GA]CATG^[CT]
657 GCATGC
PleI 591 GACTC GAGTC(4/5)
1213 GAGTC
PshAI 853 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1134 GACCAAGGTC
Tru9I 14 TTAA T^TAA
652 TTAA
Tsp509I 729 AATT ^AATT
1360 AATT
XcmI 480 CCAGAACATCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1345 CCAAACCCATACTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BalI BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MspI MvaI MvnI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoII