################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BbrPI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaI BseAI BsiEI BsiWI BsmBI BsoBI BspEI BspLU11I BspMI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRV FbaI FseI FspI HindIII HpaI KpnI KspI MfeI MroI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### AatII 460 GACGTC GACGT^C AcsI 1359 GAATTC [GA]^AATT[CT] AflIII 147 ACGCGT A^C[GA][CT]GT AgeI 781 ACCGGT A^CCGGT AlwNI 421 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1151 AGCGCT AGC^GCT ApoI 1359 GAATTC [GA]^AATT[CT] AspHI 955 GAGCTC G[AT]GC[AT]^C AspI 99 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1026 GGATCC G^GATCC BbeI 1293 GGCGCC GGCGC^C BbsI 850 GAAGAC GAAGAC(2/6) BbvCI 154 CCTCAGC CCTCAGC(-5/-2) BcgI 368 GCAGAGTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 1092 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1092 GCTCAGC GC^T[ACGT]AGC BpuAI 850 GAAGAC GAAGAC(2/6) BsaBI 871 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 781 ACCGGT [AT]^CCGG[AT] BsgI 677 GTGCAG GTGCAG(16/14) BsiHKAI 955 GAGCTC G[AT]GC[AT]^C BsmFI 151 GTCCC GGGAC(10/14) BsmI 250 GAATGC GAATGC(1/-1) Bsp1407I 1223 TGTACA T^GTACA BspDI 487 ATCGAT AT^CGAT BspHI 1130 TCATGA T^CATGA BsrBI 1057 GAGCGG CCGCTC(-3/-3) BsrFI 781 ACCGGT [GA]^CCGG[CT] BsrGI 1223 TGTACA T^GTACA BstEII 189 GGTCACC G^GT[ACGT]ACC BstPI 189 GGTCACC G^GT[ACGT]ACC CelII 1092 GCTCAGC GC^T[ACGT]AGC Cfr10I 781 ACCGGT [GA]^CCGG[CT] ClaI 487 ATCGAT AT^CGAT DraII 1190 AGGCCCT [GA]G^G[ACGT]CC[CT] Eco47III 1151 AGCGCT AGC^GCT EcoO109I 1190 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 189 GGTCACC G^GT[ACGT]ACC EcoRI 1359 GAATTC G^AATTC EcoT22I 655 ATGCAT ATGCA^T FauI 269 GCGGG CCCGC(4/6) HincII 463 GTCAAC GT[CT]^[GA]AC HindII 463 GTCAAC GT[CT]^[GA]AC KasI 1293 GGCGCC G^GCGCC MaeII 461 ACGT A^CGT MamI 871 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 147 ACGCGT A^CGCGT MslI 195 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 981 CAGCTG C[AC]G^C[GT]G NarI 1293 GGCGCC GG^CGCC NcoI 1143 CCATGG C^CATGG NsiI 655 ATGCAT ATGCA^T PflFI 99 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 916 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 781 ACCGGT A^CCGGT PvuII 981 CAGCTG CAG^CTG RcaI 1130 TCATGA T^CATGA SacI 955 GAGCTC GAGCT^C SapI 1062 GAAGAGC GCTCTTC(1/4) SfaNI 986 GCATC GCATC(5/9) SfoI 1293 GGCGCC GGC^GCC SmlI 231 CTCAAG C^T[CT][GA]AG SphI 657 GCATGC GCATG^C SspBI 1223 TGTACA T^GTACA TfiI 882 GATTC G^A[AT]TC Tth111I 99 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 916 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 460 GACGTC G[GA]^CG[CT]C 1293 GGCGCC BanI 1000 GGCACC G^G[CT][GA]CC 1293 GGCGCC BcnI 1043 CCCGG CC^[GC]GG 1209 CCCGG BsaHI 460 GACGTC G[GA]^CG[CT]C 1293 GGCGCC BsmAI 589 GAGAC GTCTC(1/5) 171 GTCTC BstF5I 558 CATCC GGATG(2/0) 302 GGATG BstXI 194 CCATCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 219 CCAGGTGAATGG EarI 596 CTCTTC CTCTTC(1/4) 1062 GAAGAG FokI 558 CATCC GGATG(9/13) 302 GGATG Hin1I 460 GACGTC G[GA]^CG[CT]C 1293 GGCGCC Ksp632I 596 CTCTTC CTCTTC(1/4) 1062 GAAGAG MseI 14 TTAA T^TAA 652 TTAA MwoI 159 GCAGGATTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 417 GCGGCAGTGGC NciI 1043 CCCGG CC^[GC]GG 1209 CCCGG NdeI 752 CATATG CA^TATG 1408 CATATG NspI 335 ACATGC [GA]CATG^[CT] 657 GCATGC PleI 591 GACTC GAGTC(4/5) 1213 GAGTC PshAI 853 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1134 GACCAAGGTC Tru9I 14 TTAA T^TAA 652 TTAA Tsp509I 729 AATT ^AATT 1360 AATT XcmI 480 CCAGAACATCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1345 CCAAACCCATACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BalI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MspI MvaI MvnI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII