Restriction map for
pF1KB7713
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AlwI AocI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
Bst1107I BstBI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfoI SfuI SgrAI SnaBI Sse8387I
SspBI SspI SwaI TfiI Tru9I
XbaI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 457 CGCG CG^CG
AhdI 766 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1018 GTGCAC G^TGCAC
Aor51HI 824 AGCGCT AGC^GCT
ApaLI 1018 GTGCAC G^TGCAC
AspEI 766 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 564 GAAGAC GAAGAC(2/6)
BbvCI 859 GCTGAGG CCTCAGC(-5/-2)
BfaI 934 CTAG C^TAG
BpuAI 564 GAAGAC GAAGAC(2/6)
BsaI 88 GAGACC GGTCTC(1/5)
BsgI 1063 CTGCAC GTGCAG(16/14)
BsmBI 378 GAGACG CGTCTC(1/5)
BsmFI 248 GGGAC GGGAC(10/14)
BsmI 582 GAATGC GAATGC(1/-1)
BssSI 1066 CACGAG CACGAG(-5/-1)
BstEII 711 GGTCACC G^GT[ACGT]ACC
BstPI 711 GGTCACC G^GT[ACGT]ACC
BstUI 457 CGCG CG^CG
DpnII 366 GATC ^GATC
DraIII 408 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 671 CCGTGG C^C[GA][CT]GG
Eam1105I 766 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 824 AGCGCT AGC^GCT
EcoO65I 711 GGTCACC G^GT[ACGT]ACC
EcoT14I 992 CCTTGG C^C[AT][AT]GG
HinfI 1012 GACTC G^A[ACGT]TC
MaeI 934 CTAG C^TAG
MaeII 251 ACGT A^CGT
MboI 366 GATC ^GATC
MvnI 457 CGCG CG^CG
NdeII 366 GATC ^GATC
NspI 428 GCATGC [GA]CATG^[CT]
PaeR7I 1252 CTCGAG C^TCGAG
PleI 1012 GACTC GAGTC(4/5)
Sau3AI 366 GATC ^GATC
ScaI 777 AGTACT AGT^ACT
SmaI 516 CCCGGG CCC^GGG
SpeI 933 ACTAGT A^CTAGT
SphI 428 GCATGC GCATG^C
StyI 992 CCTTGG C^C[AT][AT]GG
Tsp509I 1006 AATT ^AATT
XcmI 5 CCAGCCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1252 CTCGAG C^TCGAG
XmaCI 516 CCCGGG C^CCGGG
XmaI 516 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
ApaI 519 GGGCCC GGGCC^C
535 GGGCCC
AspHI 208 GTGCTC G[AT]GC[AT]^C
1018 GTGCAC
AspI 454 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
865 GACTTAGTC
AvaI 516 CCCGGG C^[CT]CG[GA]G
1252 CTCGAG
BanI 659 GGCACC G^G[CT][GA]CC
1221 GGTGCC
BanII 519 GGGCCC G[GA]GC[CT]^C
535 GGGCCC
BlpI 210 GCTCAGC GC^T[ACGT]AGC
1117 GCTGAGC
Bpu1102I 210 GCTCAGC GC^T[ACGT]AGC
1117 GCTGAGC
BsiEI 490 CGGCCG CG[GA][CT]^CG
1092 CGGCCG
BsiHKAI 208 GTGCTC G[AT]GC[AT]^C
1018 GTGCAC
BsoBI 516 CCCGGG C^[CT]CG[GA]G
1252 CTCGAG
BsrBI 1156 CCGCTC CCGCTC(-3/-3)
1120 GAGCGG
BsrFI 48 GCCGGC [GA]^CCGG[CT]
1090 GCCGGC
BstXI 5 CCAGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1191 CCATTTCTATGG
CelII 210 GCTCAGC GC^T[ACGT]AGC
1117 GCTGAGC
Cfr10I 48 GCCGGC [GA]^CCGG[CT]
1090 GCCGGC
EagI 490 CGGCCG C^GGCCG
1092 CGGCCG
EarI 154 GAAGAG CTCTTC(1/4)
880 GAAGAG
EclXI 490 CGGCCG C^GGCCG
1092 CGGCCG
Eco52I 490 CGGCCG C^GGCCG
1092 CGGCCG
HaeII 227 AGCGCC [GA]GCGC^[CT]
824 AGCGCT
HgaI 380 GACGC GACGC(5/10)
458 GCGTC
Ksp632I 154 GAAGAG CTCTTC(1/4)
880 GAAGAG
MaeIII 686 GTGAC ^GT[ACGT]AC
712 GTCAC
MslI 268 CACTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1192 CATTTCTATG
NaeI 48 GCCGGC GCC^GGC
1090 GCCGGC
NgoMIV 48 GCCGGC G^CCGGC
1090 GCCGGC
PflFI 454 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
865 GACTTAGTC
PflMI 9 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
716 CCATCAGCTGG
SfiI 387 GGCCTGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1093 GGCCGGGCTGGCC
SmlI 433 CTCAAG C^T[CT][GA]AG
1252 CTCGAG
Tsp45I 686 GTGAC ^GT[GC]AC
712 GTCAC
Tth111I 454 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
865 GACTTAGTC
Van91I 9 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
716 CCATCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PstI PvuII
RsaI Sau96I ScrFI SfaNI SfcI
StuI TaqI TseI TspRI TthHB8I