Restriction map for
pF1KB6864
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BamHI BanII BbrPI BbsI
BbvCI BcgI BclI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MflI
MluI MslI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 75 enzymes) #
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AccII 144 CGCG CG^CG
AccIII 545 TCCGGA T^CCGGA
AcyI 3 GGCGCC G[GA]^CG[CT]C
AflII 283 CTTAAG C^TTAAG
AlwNI 184 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 401 CCTTAGG CC^T[ACGT]AGG
AspI 376 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 272 GGTCC G^G[AT]CC
BalI 386 TGGCCA TGG^CCA
BbeI 3 GGCGCC GGCGC^C
BciVI 482 GTATCC GTATCC(6/5)
BfaI 227 CTAG C^TAG
BfrI 283 CTTAAG C^TTAAG
BmyI 519 GTGCCC G[AGT]GC[ACT]^C
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BsaI 222 GGTCTC GGTCTC(1/5)
BsaWI 545 TCCGGA [AT]^CCGG[AT]
BseAI 545 TCCGGA T^CCGGA
BseRI 52 GAGGAG GAGGAG(10/8)
BsmAI 223 GTCTC GTCTC(1/5)
BsmFI 180 GTCCC GGGAC(10/14)
Bsp1286I 519 GTGCCC G[AGT]GC[ACT]^C
BspEI 545 TCCGGA T^CCGGA
BspMI 425 ACCTGC ACCTGC(4/8)
BsrFI 112 GCCGGC [GA]^CCGG[CT]
BssHII 143 GCGCGC G^CGCGC
BstNI 370 CCAGG CC^[AT]GG
BstUI 144 CGCG CG^CG
Bsu36I 401 CCTTAGG CC^T[ACGT]AGG
Cfr10I 112 GCCGGC [GA]^CCGG[CT]
Cfr13I 272 GGTCC G^G[ACGT]CC
DpnII 396 GATC ^GATC
DsaI 87 CCACGG C^C[GA][CT]GG
EaeI 386 TGGCCA [CT]^GGCC[GA]
EarI 126 GAAGAG CTCTTC(1/4)
Eco81I 401 CCTTAGG CC^T[ACGT]AGG
EcoRII 370 CCAGG ^CC[AT]GG
FauI 83 CCCGC CCCGC(4/6)
HaeII 3 GGCGCC [GA]GCGC^[CT]
HaeIII 387 GGCC GG^CC
Hin1I 3 GGCGCC G[GA]^CG[CT]C
KasI 3 GGCGCC G^GCGCC
Ksp632I 126 GAAGAG CTCTTC(1/4)
MaeI 227 CTAG C^TAG
MaeII 36 ACGT A^CGT
MboI 396 GATC ^GATC
MluNI 386 TGGCCA TGG^CCA
MroI 545 TCCGGA T^CCGGA
MscI 386 TGGCCA TGG^CCA
MseI 284 TTAA T^TAA
MvaI 370 CCAGG CC^[AT]GG
MvnI 144 CGCG CG^CG
NaeI 112 GCCGGC GCC^GGC
NarI 3 GGCGCC GG^CGCC
NdeII 396 GATC ^GATC
NgoMIV 112 GCCGGC G^CCGGC
NlaIII 276 CATG CATG^
PflFI 376 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 497 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 501 CAGCTG CAG^CTG
SapI 126 GAAGAGC GCTCTTC(1/4)
Sau3AI 396 GATC ^GATC
Sau96I 272 GGTCC G^G[ACGT]CC
SfcI 161 CTGTAG C^T[GA][CT]AG
SfoI 3 GGCGCC GGC^GCC
SmaI 63 CCCGGG CCC^GGG
SmlI 283 CTTAAG C^T[CT][GA]AG
TaqI 297 TCGA T^CGA
Tru9I 284 TTAA T^TAA
Tsp45I 374 GTGAC ^GT[GC]AC
Tth111I 376 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 297 TCGA T^CGA
Van91I 497 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 63 CCCGGG C^CCGGG
XmaI 63 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 253 GTAC GT^AC
562 GTAC
AvaI 63 CCCGGG C^[CT]CG[GA]G
557 CCCGAG
BanI 3 GGCGCC G^G[CT][GA]CC
73 GGCACC
BsoBI 63 CCCGGG C^[CT]CG[GA]G
557 CCCGAG
BsrI 151 CCAGT ACTGG(1/-1)
183 CCAGT
BstF5I 24 CATCC GGATG(2/0)
258 CATCC
DdeI 402 CTTAG C^T[ACGT]AG
524 CTAAG
EcoRI 541 GAATTC G^AATTC
571 GAATTC
FokI 24 CATCC GGATG(9/13)
258 CATCC
HinfI 178 GAGTC G^A[ACGT]TC
475 GAGTC
HphI 373 GGTGA GGTGA(8/7)
406 GGTGA
MboII 126 GAAGA GAAGA(8/7)
316 GAAGA
MspA1I 69 CAGCGG C[AC]G^C[GT]G
501 CAGCTG
PleI 178 GAGTC GAGTC(4/5)
475 GAGTC
RsaI 253 GTAC GT^AC
562 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BcnI BsaJI BsiYI BslI BssKI
Cac8I CfoI Fnu4HI HapII HhaI
HinP1I HpaII ItaI MaeIII MnlI
MspI MwoI NciI NlaIV ScrFI
SfaNI TseI Tsp509I TspRI