Restriction map for
pF1KE0507
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
BamHI BanI BbeI BbrPI BbsI
BbvCI BciVI BclI BfrI BglII
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII Hin1I HincII HindII HpaI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MflI MluI MroI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 382 GTAC GT^AC
AviII 71 TGCGCA TGC^GCA
AvrII 627 CCTAGG C^CTAGG
BcgI 246 CGAGGAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 628 CTAG C^TAG
BlnI 627 CCTAGG C^CTAGG
BsaI 658 GAGACC GGTCTC(1/5)
BsgI 213 CTGCAC GTGCAG(16/14)
BsmAI 658 GAGAC GTCTC(1/5)
BsrBI 438 CCGCTC CCGCTC(-3/-3)
BsrFI 315 GCCGGC [GA]^CCGG[CT]
BstF5I 443 CATCC GGATG(2/0)
Cfr10I 315 GCCGGC [GA]^CCGG[CT]
DpnII 702 GATC ^GATC
EarI 729 GAAGAG CTCTTC(1/4)
FokI 443 CATCC GGATG(9/13)
FspI 71 TGCGCA TGC^GCA
HgaI 409 GACGC GACGC(5/10)
HindIII 357 AAGCTT A^AGCTT
HphI 336 GGTGA GGTGA(8/7)
Ksp632I 729 GAAGAG CTCTTC(1/4)
MaeI 628 CTAG C^TAG
MboI 702 GATC ^GATC
NaeI 315 GCCGGC GCC^GGC
NdeII 702 GATC ^GATC
NgoMIV 315 GCCGGC G^CCGGC
NruI 171 TCGCGA TCG^CGA
NspI 368 ACATGC [GA]CATG^[CT]
PflMI 417 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 91 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 382 GTAC GT^AC
Sau3AI 702 GATC ^GATC
SfiI 321 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 350 CCCGGG CCC^GGG
SmlI 139 CTCAAG C^T[CT][GA]AG
StuI 329 AGGCCT AGG^CCT
Van91I 417 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 350 CCCGGG C^CCGGG
XmaI 350 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 172 CGCG CG^CG
385 CGCG
AlwNI 418 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
462 CAGCCGCTG
AvaI 244 CCCGAG C^[CT]CG[GA]G
350 CCCGGG
AvaII 54 GGACC G^G[AT]CC
92 GGACC
BalI 513 TGGCCA TGG^CCA
693 TGGCCA
BglI 322 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
539 GCCCTGTGGGC
BpmI 281 CTGGAG CTGGAG(16/14)
505 CTGGAG
BsoBI 244 CCCGAG C^[CT]CG[GA]G
350 CCCGGG
BstUI 172 CGCG CG^CG
385 CGCG
EcoNI 522 CCTCTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
627 CCTAGGCAAGG
EcoT14I 527 CCAAGG C^C[AT][AT]GG
627 CCTAGG
FauI 499 GCGGG CCCGC(4/6)
562 GCGGG
HinfI 43 GACTC G^A[ACGT]TC
168 GAGTC
MluNI 513 TGGCCA TGG^CCA
693 TGGCCA
MscI 513 TGGCCA TGG^CCA
693 TGGCCA
MspA1I 50 CAGCGG C[AC]G^C[GT]G
465 CCGCTG
MvnI 172 CGCG CG^CG
385 CGCG
PleI 43 GACTC GAGTC(4/5)
168 GAGTC
SfaNI 125 GATGC GCATC(5/9)
274 GATGC
StyI 527 CCAAGG C^C[AT][AT]GG
627 CCTAGG
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI ApaI BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
PstI Sau96I ScrFI SfcI TseI
TspRI