Restriction map for
pF1KE0166
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwNI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
EclXI Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 576 CGCG CG^CG
AfaI 149 GTAC GT^AC
AhdI 516 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 65 GATCC GGATC(4/5)
AocI 57 CCTGAGG CC^T[ACGT]AGG
AspEI 516 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 91 GGGCTC G[GA]GC[CT]^C
BmyI 91 GGGCTC G[AGT]GC[ACT]^C
BseRI 60 GAGGAG GAGGAG(10/8)
BsgI 209 CTGCAC GTGCAG(16/14)
Bsp1286I 91 GGGCTC G[AGT]GC[ACT]^C
BsrDI 235 GCAATG GCAATG(2/0)
BstUI 576 CGCG CG^CG
BstYI 64 AGATCC [GA]^GATC[CT]
Bsu36I 57 CCTGAGG CC^T[ACGT]AGG
DsaI 87 CCATGG C^C[GA][CT]GG
Eam1105I 516 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 57 CCTGAGG CC^T[ACGT]AGG
EcoNI 102 CCTTTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 651 CCCGC CCCGC(4/6)
HaeIII 184 GGCC GG^CC
HapII 298 CCGG C^CGG
HpaII 298 CCGG C^CGG
MflI 64 AGATCC [GA]^GATC[CT]
MseI 494 TTAA T^TAA
MslI 717 CATCTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 652 CCGCTG C[AC]G^C[GT]G
MspI 298 CCGG C^CGG
MvnI 576 CGCG CG^CG
NcoI 87 CCATGG C^CATGG
NlaIV 52 GGAACC GG[ACGT]^[ACGT]CC
NspI 691 GCATGC [GA]CATG^[CT]
RsaI 149 GTAC GT^AC
SapI 707 GCTCTTC GCTCTTC(1/4)
SfaNI 571 GATGC GCATC(5/9)
SphI 691 GCATGC GCATG^C
StuI 183 AGGCCT AGG^CCT
TfiI 368 GATTC G^A[AT]TC
Tru9I 494 TTAA T^TAA
XhoII 64 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AluI 20 AGCT AG^CT
265 AGCT
Aor51HI 73 AGCGCT AGC^GCT
667 AGCGCT
AvaII 24 GGACC G^G[AT]CC
487 GGTCC
Cac8I 654 GCTGGC GC[ACGT]^[ACGT]GC
691 GCATGC
Cfr13I 24 GGACC G^G[ACGT]CC
487 GGTCC
EarI 538 CTCTTC CTCTTC(1/4)
708 CTCTTC
Eco47III 73 AGCGCT AGC^GCT
667 AGCGCT
EcoT14I 87 CCATGG C^C[AT][AT]GG
543 CCAAGG
HaeII 73 AGCGCT [GA]GCGC^[CT]
667 AGCGCT
Ksp632I 538 CTCTTC CTCTTC(1/4)
708 CTCTTC
Sau96I 24 GGACC G^G[ACGT]CC
487 GGTCC
SmlI 28 CTTGAG C^T[CT][GA]AG
267 CTTGAG
StyI 87 CCATGG C^C[AT][AT]GG
543 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI BbvI BpmI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI CfoI DdeI DpnII EcoRII
Fnu4HI FokI HhaI HinP1I HinfI
HphI ItaI MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
PleI Sau3AI ScrFI TseI Tsp45I
Tsp509I TspRI