Restriction map for
pF1KE0135
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbrPI
BbsI BcgI BciVI BcnI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaWI BseAI BsgI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BspMI BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII Eam1105I Eco47III Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I HpaI
KpnI KspI MaeI MfeI MluI
MroI MseI MunI NciI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tru9I
Tsp509I Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Asp700I 705 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 175 GAGCTC G[AT]GC[AT]^C
AvaI 691 CTCGAG C^[CT]CG[GA]G
AviII 150 TGCGCA TGC^GCA
BalI 263 TGGCCA TGG^CCA
BanII 175 GAGCTC G[GA]GC[CT]^C
BbeI 16 GGCGCC GGCGC^C
BclI 767 TGATCA T^GATCA
BglII 347 AGATCT A^GATCT
BsaBI 594 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 33 CGGCCG CG[GA][CT]^CG
BsiHKAI 175 GAGCTC G[AT]GC[AT]^C
BsoBI 691 CTCGAG C^[CT]CG[GA]G
Bsp1407I 749 TGTACA T^GTACA
BsrBI 247 GAGCGG CCGCTC(-3/-3)
BsrGI 749 TGTACA T^GTACA
BssKI 369 CCTGG ^CC[ACGT]GG
BssSI 199 CTCGTG CACGAG(-5/-1)
BstAPI 672 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 369 CCTGG CC^[AT]GG
BstXI 185 CCAGCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 796 GACTTCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 191 CCGTGG C^C[GA][CT]GG
EagI 33 CGGCCG C^GGCCG
EclXI 33 CGGCCG C^GGCCG
Eco52I 33 CGGCCG C^GGCCG
EcoRII 369 CCTGG ^CC[AT]GG
FbaI 767 TGATCA T^GATCA
FseI 30 GGCCGGCC GGCCGG^CC
FspI 150 TGCGCA TGC^GCA
HincII 484 GTTGAC GT[CT]^[GA]AC
HindII 484 GTTGAC GT[CT]^[GA]AC
KasI 16 GGCGCC G^GCGCC
MaeII 482 ACGT A^CGT
MamI 594 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 263 TGGCCA TGG^CCA
MscI 263 TGGCCA TGG^CCA
MslI 853 CATGACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 369 CCTGG CC^[AT]GG
NaeI 31 GCCGGC GCC^GGC
NarI 16 GGCGCC GG^CGCC
NgoMIV 31 GCCGGC G^CCGGC
PaeR7I 691 CTCGAG C^TCGAG
PflMI 962 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 898 GAGTC GAGTC(4/5)
PvuII 259 CAGCTG CAG^CTG
SacI 175 GAGCTC GAGCT^C
SapI 541 GAAGAGC GCTCTTC(1/4)
ScrFI 369 CCTGG CC^[ACGT]GG
SfaNI 101 GCATC GCATC(5/9)
SfoI 16 GGCGCC GGC^GCC
SgrAI 631 CACCGGCG C[GA]^CCGG[CT]G
SspBI 749 TGTACA T^GTACA
TfiI 528 GAATC G^A[AT]TC
Van91I 962 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 691 CTCGAG C^TCGAG
XmnI 705 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 16 GGCGCC G[GA]^CG[CT]C
877 GACGCC
AlwI 696 GATCC GGATC(4/5)
1011 GATCC
AvaII 725 GGTCC G^G[AT]CC
829 GGACC
BanI 16 GGCGCC G^G[CT][GA]CC
629 GGCACC
BbvCI 720 GCTGAGG CCTCAGC(-5/-2)
921 GCTGAGG
BmyI 175 GAGCTC G[AGT]GC[ACT]^C
628 GGGCAC
BsaHI 16 GGCGCC G[GA]^CG[CT]C
877 GACGCC
BsaI 509 GAGACC GGTCTC(1/5)
196 GGTCTC
BsmAI 509 GAGAC GTCTC(1/5)
197 GTCTC
BsmFI 998 GGGAC GGGAC(10/14)
900 GTCCC
Bsp1286I 175 GAGCTC G[AGT]GC[ACT]^C
628 GGGCAC
BspHI 119 TCATGA T^CATGA
852 TCATGA
BsrFI 31 GCCGGC [GA]^CCGG[CT]
632 ACCGGC
BsrI 465 ACTGG ACTGG(1/-1)
614 ACTGG
BstYI 347 AGATCT [GA]^GATC[CT]
695 AGATCC
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
632 ACCGGC
Cfr13I 725 GGTCC G^G[ACGT]CC
829 GGACC
EcoNI 369 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
678 CCTGCAGAAGG
EcoT14I 281 CCAAGG C^C[AT][AT]GG
809 CCAAGG
HaeII 16 GGCGCC [GA]GCGC^[CT]
687 GGCGCT
HapII 32 CCGG C^CGG
633 CCGG
HgaI 160 GACGC GACGC(5/10)
877 GACGC
Hin1I 16 GGCGCC G[GA]^CG[CT]C
877 GACGCC
HindIII 307 AAGCTT A^AGCTT
492 AAGCTT
HinfI 528 GAATC G^A[ACGT]TC
898 GAGTC
HpaII 32 CCGG C^CGG
633 CCGG
MflI 347 AGATCT [GA]^GATC[CT]
695 AGATCC
MspA1I 259 CAGCTG C[AC]G^C[GT]G
966 CCGCTG
MspI 32 CCGG C^CGG
633 CCGG
PstI 289 CTGCAG CTGCA^G
679 CTGCAG
RcaI 119 TCATGA T^CATGA
852 TCATGA
Sau96I 725 GGTCC G^G[ACGT]CC
829 GGACC
SfcI 289 CTGCAG C^T[GA][CT]AG
679 CTGCAG
SmlI 178 CTCAAG C^T[CT][GA]AG
691 CTCGAG
StyI 281 CCAAGG C^C[AT][AT]GG
809 CCAAGG
XhoII 347 AGATCT [GA]^GATC[CT]
695 AGATCC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
BbvI BpmI BsaJI BseRI BsiYI
BslI BstF5I BstUI Cac8I CfoI
DdeI DpnII EaeI EarI FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MvnI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI TaqI
TseI Tsp45I TspRI TthHB8I