Restriction map for
pF1KB6517
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AvaI
AviII AvrII BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI ItaI
KasI KpnI KspI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI SwaI TseI
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 59 GTCTAC GT^[AC][GT]AC
AhdI 801 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 14 GTGCAC G^TGCAC
ApaLI 14 GTGCAC G^TGCAC
AspEI 801 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 14 GTGCAC G[AT]GC[AT]^C
AspI 874 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 443 GGACC G^G[AT]CC
BalI 246 TGGCCA TGG^CCA
BamHI 586 GGATCC G^GATCC
BcnI 440 CCCGG CC^[GC]GG
BsaBI 587 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 14 GTGCAC G[AT]GC[AT]^C
BsiYI 1074 CCAAAGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1074 CCAAAGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 293 GGGAC GGGAC(10/14)
BsmI 547 GAATGC GAATGC(1/-1)
BsrI 105 ACTGG ACTGG(1/-1)
BstBI 827 TTCGAA TT^CGAA
BstXI 176 CCAAGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 443 GGACC G^G[ACGT]CC
DraIII 567 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 295 GACTGCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 246 TGGCCA [CT]^GGCC[GA]
Eam1105I 801 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 651 GAAGAG CTCTTC(1/4)
HapII 441 CCGG C^CGG
HincII 760 GTTGAC GT[CT]^[GA]AC
HindII 760 GTTGAC GT[CT]^[GA]AC
HindIII 1077 AAGCTT A^AGCTT
HpaII 441 CCGG C^CGG
Ksp632I 651 GAAGAG CTCTTC(1/4)
MaeII 118 ACGT A^CGT
MamI 587 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 246 TGGCCA TGG^CCA
MscI 246 TGGCCA TGG^CCA
MslI 238 CATCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 441 CCGG C^CGG
NciI 440 CCCGG CC^[GC]GG
NspI 673 ACATGC [GA]CATG^[CT]
NspV 827 TTCGAA TT^CGAA
PflFI 874 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1074 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 443 GGACC G^G[ACGT]CC
SfcI 206 CTACAG C^T[GA][CT]AG
SfuI 827 TTCGAA TT^CGAA
SpeI 1036 ACTAGT A^CTAGT
StuI 231 AGGCCT AGG^CCT
TaqI 828 TCGA T^CGA
Tth111I 874 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 828 TCGA T^CGA
Van91I 1074 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AluI 288 AGCT AG^CT
1078 AGCT
AlwI 587 GATCC GGATC(4/5)
586 GGATC
BglII 592 AGATCT A^GATCT
969 AGATCT
BmyI 14 GTGCAC G[AGT]GC[ACT]^C
328 GTGCCC
BpmI 272 CTCCAG CTGGAG(16/14)
791 CTGGAG
BsmAI 79 GAGAC GTCTC(1/5)
214 GAGAC
Bsp1286I 14 GTGCAC G[AGT]GC[ACT]^C
328 GTGCCC
BstF5I 1020 CATCC GGATG(2/0)
312 GGATG
BstNI 33 CCAGG CC^[AT]GG
274 CCAGG
EcoRII 33 CCAGG ^CC[AT]GG
274 CCAGG
EcoT14I 176 CCAAGG C^C[AT][AT]GG
699 CCAAGG
FokI 1020 CATCC GGATG(9/13)
312 GGATG
HaeIII 232 GGCC GG^CC
247 GGCC
MvaI 33 CCAGG CC^[AT]GG
274 CCAGG
MwoI 248 GCCAATTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
326 GCGTGCCCTGC
NlaIV 443 GGACCC GG[ACGT]^[ACGT]CC
586 GGATCC
SfaNI 237 GCATC GCATC(5/9)
924 GCATC
SmlI 765 CTTGAG C^T[CT][GA]AG
1024 CTCAAG
StyI 176 CCAAGG C^C[AT][AT]GG
699 CCAAGG
TfiI 536 GAATC G^A[AT]TC
712 GAATC
TspRI 505 CACTG CA[GC]TG[ACGT][ACGT]^
737 CAGTG
XcmI 590 CCAGATCTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
634 CCACTAATTGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AcsI AfaI ApoI BbsI BfaI
BpuAI BsaJI BsgI BspHI BssKI
BstYI Cac8I DdeI DpnII HinfI
HphI MaeI MaeIII MboI MboII
MflI MnlI MseI NdeII NlaIII
PleI RcaI RsaI Sau3AI ScrFI
Tru9I Tsp45I Tsp509I XhoII