Restriction map for
pF1KB6358
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MfeI MluI MluNI
MroI MscI MunI NcoI NdeI
NheI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SexAI SfaNI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI StyI SwaI
TfiI Tsp509I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 829 GTCTAC GT^[AC][GT]AC
ApaI 851 GGGCCC GGGCC^C
Asp700I 498 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 823 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 99 GGACC G^G[AT]CC
BglII 755 AGATCT A^GATCT
BsaBI 750 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 202 GTGCAG GTGCAG(16/14)
BsmBI 8 GAGACG CGTCTC(1/5)
BsmFI 428 GGGAC GGGAC(10/14)
BsrBI 88 GAGCGG CCGCTC(-3/-3)
BssSI 679 CACGAG CACGAG(-5/-1)
BstF5I 749 GGATG GGATG(2/0)
BstYI 755 AGATCT [GA]^GATC[CT]
DpnII 756 GATC ^GATC
DraII 850 GGGGCCC [GA]G^G[ACGT]CC[CT]
EarI 388 GAAGAG CTCTTC(1/4)
EcoO109I 850 GGGGCCC [GA]G^G[ACGT]CC[CT]
FokI 749 GGATG GGATG(9/13)
Ksp632I 388 GAAGAG CTCTTC(1/4)
MamI 750 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 756 GATC ^GATC
MboII 388 GAAGA GAAGA(8/7)
MflI 755 AGATCT [GA]^GATC[CT]
MseI 662 TTAA T^TAA
MslI 600 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 756 GATC ^GATC
NlaIII 421 CATG CATG^
NotI 121 GCGGCCGC GC^GGCCGC
PflFI 823 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 550 CTGCAG CTGCA^G
SacI 652 GAGCTC GAGCT^C
Sau3AI 756 GATC ^GATC
SbfI 549 CCTGCAGG CCTGCA^GG
ScaI 500 AGTACT AGT^ACT
SfcI 550 CTGCAG C^T[GA][CT]AG
SmaI 854 CCCGGG CCC^GGG
SmlI 268 CTCAAG C^T[CT][GA]AG
Sse8387I 549 CCTGCAGG CCTGCA^GG
StuI 206 AGGCCT AGG^CCT
TaqI 806 TCGA T^CGA
Tru9I 662 TTAA T^TAA
Tth111I 823 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 806 TCGA T^CGA
XhoII 755 AGATCT [GA]^GATC[CT]
XmaCI 854 CCCGGG C^CCGGG
XmaI 854 CCCGGG C^CCGGG
XmnI 498 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BanII 652 GAGCTC G[GA]GC[CT]^C
851 GGGCCC
BbeI 250 GGCGCC GGCGC^C
356 GGCGCC
BbvCI 79 GCTGAGG CCTCAGC(-5/-2)
556 GCTGAGG
BpmI 435 CTGGAG CTGGAG(16/14)
455 CTGGAG
BsmAI 8 GAGAC GTCTC(1/5)
458 GAGAC
BspMI 548 ACCTGC ACCTGC(4/8)
711 GCAGGT
BsrFI 186 GCCGGC [GA]^CCGG[CT]
594 GCCGGC
BstEII 13 GGTGACC G^GT[ACGT]ACC
858 GGTGACC
BstPI 13 GGTGACC G^GT[ACGT]ACC
858 GGTGACC
Cfr10I 186 GCCGGC [GA]^CCGG[CT]
594 GCCGGC
EcoO65I 13 GGTGACC G^GT[ACGT]ACC
858 GGTGACC
HaeII 250 GGCGCC [GA]GCGC^[CT]
356 GGCGCC
HinfI 588 GACTC G^A[ACGT]TC
823 GACTC
KasI 250 GGCGCC G^GCGCC
356 GGCGCC
MaeII 413 ACGT A^CGT
686 ACGT
NaeI 186 GCCGGC GCC^GGC
594 GCCGGC
NarI 250 GGCGCC GG^CGCC
356 GGCGCC
NgoMIV 186 GCCGGC G^CCGGC
594 GCCGGC
PleI 588 GACTC GAGTC(4/5)
823 GACTC
SfoI 250 GGCGCC GGC^GCC
356 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI BanI BbvI BcnI
BmyI BsaHI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI EaeI EagI
EclXI Eco52I EcoRII FauI Fnu4HI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeIII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV RsaI Sau96I
ScrFI TseI Tsp45I TspRI