Restriction map for
pF1KF0156
##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AclI AflII AflIII AgeI Aor51HI
AscI AseI AsnI AviII AvrII
BbeI BfrI BlnI BsiEI BsiWI
BspHI BspLU11I BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FseI FspI
HaeII HincII HindII HpaI KasI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI TfiI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 296 GGTACC G^GTACC
AccII 3720 CGCG CG^CG
AccIII 3983 TCCGGA T^CCGGA
AhdI 1792 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1332 GTGCAC G^TGCAC
ApaLI 1332 GTGCAC G^TGCAC
Asp718I 296 GGTACC G^GTACC
AspEI 1792 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 4952 CGAGGGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5063 GTATCC GTATCC(6/5)
BclI 3012 TGATCA T^GATCA
BsaBI 5301 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3983 TCCGGA [AT]^CCGG[AT]
BseAI 3983 TCCGGA T^CCGGA
BspDI 2473 ATCGAT AT^CGAT
BspEI 3983 TCCGGA T^CCGGA
BssSI 3420 CTCGTG CACGAG(-5/-1)
BstUI 3720 CGCG CG^CG
ClaI 2473 ATCGAT AT^CGAT
Eam1105I 1792 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3012 TGATCA T^GATCA
KpnI 296 GGTACC GGTAC^C
KspI 3719 CCGCGG CCGC^GG
MamI 5301 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3983 TCCGGA T^CCGGA
MvnI 3720 CGCG CG^CG
SacII 3719 CCGCGG CCGC^GG
SmaI 2671 CCCGGG CCC^GGG
XmaCI 2671 CCCGGG C^CCGGG
XmaI 2671 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 4105 GACGTC GACGT^C
5146 GACGTC
AcyI 4105 GACGTC G[GA]^CG[CT]C
5146 GACGTC
AocI 318 CCTGAGG CC^T[ACGT]AGG
1588 CCTGAGG
Asp700I 511 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3816 GAAGATTTTC
AvaI 2671 CCCGGG C^[CT]CG[GA]G
4950 CCCGAG
BamHI 1045 GGATCC G^GATCC
2831 GGATCC
BlpI 486 GCTCAGC GC^T[ACGT]AGC
4929 GCTCAGC
Bpu1102I 486 GCTCAGC GC^T[ACGT]AGC
4929 GCTCAGC
BsaHI 4105 GACGTC G[GA]^CG[CT]C
5146 GACGTC
BsaI 71 GGTCTC GGTCTC(1/5)
1624 GGTCTC
BsmI 543 GAATGC GAATGC(1/-1)
2137 GAATGC
BsoBI 2671 CCCGGG C^[CT]CG[GA]G
4950 CCCGAG
Bsp1407I 710 TGTACA T^GTACA
1961 TGTACA
BsrBI 2527 CCGCTC CCGCTC(-3/-3)
5040 GAGCGG
BsrDI 2239 CATTGC GCAATG(2/0)
3737 GCAATG
BsrGI 710 TGTACA T^GTACA
1961 TGTACA
Bsu36I 318 CCTGAGG CC^T[ACGT]AGG
1588 CCTGAGG
CelII 486 GCTCAGC GC^T[ACGT]AGC
4929 GCTCAGC
DrdI 3916 GACATGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5323 GACGGCATGGTC
Eco81I 318 CCTGAGG CC^T[ACGT]AGG
1588 CCTGAGG
EcoT22I 2236 ATGCAT ATGCA^T
2759 ATGCAT
HgaI 3216 GACGC GACGC(5/10)
5014 GCGTC
Hin1I 4105 GACGTC G[GA]^CG[CT]C
5146 GACGTC
HindIII 3567 AAGCTT A^AGCTT
4029 AAGCTT
NsiI 2236 ATGCAT ATGCA^T
2759 ATGCAT
SapI 1098 GAAGAGC GCTCTTC(1/4)
4065 GAAGAGC
SbfI 936 CCTGCAGG CCTGCA^GG
3477 CCTGCAGG
SexAI 3251 ACCTGGT A^CC[AT]GGT
3977 ACCTGGT
SphI 951 GCATGC GCATG^C
4181 GCATGC
Sse8387I 936 CCTGCAGG CCTGCA^GG
3477 CCTGCAGG
SspBI 710 TGTACA T^GTACA
1961 TGTACA
XmnI 511 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3816 GAAGATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AspI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII