Restriction map for
pF1KF0414
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# NO CUT ENZYMES ( 83 enzymes) #
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Acc65I AccI AclI AcsI AflII
AgeI ApoI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BbvCI
BclI BfrI BglII BlnI BsaBI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MseI MunI NdeI NheI
NotI NruI NspV PacI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI ScaI SfuI SpeI SphI
SspBI SspI SwaI Tru9I Tsp509I
Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 733 GACGTC GACGT^C
AhdI 1216 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2896 CCTGAGG CC^T[ACGT]AGG
Aor51HI 332 AGCGCT AGC^GCT
AspEI 1216 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3084 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2505 GGCGCC GGCGC^C
BbrPI 2131 CACGTG CAC^GTG
BbsI 1392 GAAGAC GAAGAC(2/6)
BcgI 932 GCAAGATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3120 GTATCC GTATCC(6/5)
BfaI 2140 CTAG C^TAG
BlpI 534 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 534 GCTGAGC GC^T[ACGT]AGC
BpuAI 1392 GAAGAC GAAGAC(2/6)
BsgI 133 CTGCAC GTGCAG(16/14)
BsmBI 1161 CGTCTC CGTCTC(1/5)
BspLU11I 611 ACATGT A^CATGT
BsrBI 2653 GAGCGG CCGCTC(-3/-3)
BsrDI 1253 GCAATG GCAATG(2/0)
Bsu36I 2896 CCTGAGG CC^T[ACGT]AGG
CelII 534 GCTGAGC GC^T[ACGT]AGC
DraIII 505 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1216 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 332 AGCGCT AGC^GCT
Eco81I 2896 CCTGAGG CC^T[ACGT]AGG
EcoNI 939 CCTCGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 1275 AAGCTT A^AGCTT
KasI 2505 GGCGCC G^GCGCC
MaeI 2140 CTAG C^TAG
NarI 2505 GGCGCC GG^CGCC
NcoI 696 CCATGG C^CATGG
PaeR7I 373 CTCGAG C^TCGAG
PflFI 3084 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2131 CACGTG CAC^GTG
PmlI 2131 CACGTG CAC^GTG
SapI 2526 GAAGAGC GCTCTTC(1/4)
SexAI 1424 ACCTGGT A^CC[AT]GGT
SfiI 2218 GGCCTAAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2505 GGCGCC GGC^GCC
SgrAI 2622 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 1735 TACGTA TAC^GTA
Tth111I 3084 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2058 CCACGATCCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 373 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccIII 1826 TCCGGA T^CCGGA
2454 TCCGGA
BamHI 2312 GGATCC G^GATCC
2614 GGATCC
BsaAI 1735 TACGTA [CT]AC^GT[GA]
2131 CACGTG
BsaI 1507 GAGACC GGTCTC(1/5)
1996 GAGACC
BsaWI 1826 TCCGGA [AT]^CCGG[AT]
2454 TCCGGA
BseAI 1826 TCCGGA T^CCGGA
2454 TCCGGA
BspEI 1826 TCCGGA T^CCGGA
2454 TCCGGA
BstAPI 126 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1815 GCAGCTGGTGC
BstEII 580 GGTGACC G^GT[ACGT]ACC
3059 GGTCACC
BstPI 580 GGTGACC G^GT[ACGT]ACC
3059 GGTCACC
BstXI 1439 CCAAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2408 CCAAGGGGGTGG
EcoO65I 580 GGTGACC G^GT[ACGT]ACC
3059 GGTCACC
EcoT22I 1055 ATGCAT ATGCA^T
2595 ATGCAT
MroI 1826 TCCGGA T^CCGGA
2454 TCCGGA
NsiI 1055 ATGCAT ATGCA^T
2595 ATGCAT
StuI 2156 AGGCCT AGG^CCT
2226 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SmaI SmlI Sse8387I StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XhoII XmaCI XmaI