Restriction map for
pF1KF0417
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BbvCI BcgI BclI
BfrI BlnI BlpI Bpu1102I BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoRI EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MfeI MluI MseI
MunI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI ScaI SexAI SfuI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tru9I Tsp509I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 948 GGCGCC G[GA]^CG[CT]C
Alw44I 457 GTGCAC G^TGCAC
AlwNI 652 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 317 AGCGCT AGC^GCT
ApaLI 457 GTGCAC G^TGCAC
AspI 1527 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 528 TGGCCA TGG^CCA
BbeI 948 GGCGCC GGCGC^C
BbrPI 574 CACGTG CAC^GTG
BbsI 310 GTCTTC GAAGAC(2/6)
BciVI 1563 GTATCC GTATCC(6/5)
BfaI 583 CTAG C^TAG
BglII 260 AGATCT A^GATCT
BpuAI 310 GTCTTC GAAGAC(2/6)
BsaAI 574 CACGTG [CT]AC^GT[GA]
BsaBI 261 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 948 GGCGCC G[GA]^CG[CT]C
BsaI 439 GAGACC GGTCTC(1/5)
BsrI 1170 ACTGG ACTGG(1/-1)
BstEII 1502 GGTCACC G^GT[ACGT]ACC
BstPI 1502 GGTCACC G^GT[ACGT]ACC
Eco47III 317 AGCGCT AGC^GCT
EcoO65I 1502 GGTCACC G^GT[ACGT]ACC
EcoT22I 1038 ATGCAT ATGCA^T
HgaI 537 GACGC GACGC(5/10)
Hin1I 948 GGCGCC G[GA]^CG[CT]C
KasI 948 GGCGCC G^GCGCC
MaeI 583 CTAG C^TAG
MaeII 575 ACGT A^CGT
MamI 261 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 528 TGGCCA TGG^CCA
MscI 528 TGGCCA TGG^CCA
NarI 948 GGCGCC GG^CGCC
NsiI 1038 ATGCAT ATGCA^T
PflFI 1527 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 574 CACGTG CAC^GTG
PmlI 574 CACGTG CAC^GTG
PvuII 953 CAGCTG CAG^CTG
SfiI 661 GGCCTAAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 948 GGCGCC GGC^GCC
SgrAI 1065 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 90 CTTGAG C^T[CT][GA]AG
Tth111I 1527 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 501 CCACGATCCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 400 CGCG CG^CG
474 CGCG
AccIII 69 TCCGGA T^CCGGA
897 TCCGGA
AfaI 579 GTAC GT^AC
833 GTAC
AflIII 573 ACACGT A^C[GA][CT]GT
575 ACGTGT
AocI 49 CCTGAGG CC^T[ACGT]AGG
1339 CCTGAGG
BsaWI 69 TCCGGA [AT]^CCGG[AT]
897 TCCGGA
BseAI 69 TCCGGA T^CCGGA
897 TCCGGA
BseRI 436 GAGGAG GAGGAG(10/8)
1408 GAGGAG
BsmAI 439 GAGAC GTCTC(1/5)
1577 GTCTC
BspEI 69 TCCGGA T^CCGGA
897 TCCGGA
BstUI 400 CGCG CG^CG
474 CGCG
BstXI 176 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
851 CCAAGGGGGTGG
Bsu36I 49 CCTGAGG CC^T[ACGT]AGG
1339 CCTGAGG
DdeI 50 CTGAG C^T[ACGT]AG
1340 CTGAG
Eco81I 49 CCTGAGG CC^T[ACGT]AGG
1339 CCTGAGG
MroI 69 TCCGGA T^CCGGA
897 TCCGGA
MslI 606 CATCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1035 CACATGCATG
MvnI 400 CGCG CG^CG
474 CGCG
NaeI 19 GCCGGC GCC^GGC
1066 GCCGGC
NgoMIV 19 GCCGGC G^CCGGC
1066 GCCGGC
RsaI 579 GTAC GT^AC
833 GTAC
SapI 969 GAAGAGC GCTCTTC(1/4)
40 GCTCTTC
SbfI 1349 CCTGCAGG CCTGCA^GG
1368 CCTGCAGG
Sse8387I 1349 CCTGCAGG CCTGCA^GG
1368 CCTGCAGG
StuI 599 AGGCCT AGG^CCT
669 AGGCCT
TaqI 269 TCGA T^CGA
338 TCGA
TthHB8I 269 TCGA T^CGA
338 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AluI AlwI ApaI AspHI
AvaI AvaII BamHI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BssKI BssSI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TseI
Tsp45I TspRI XhoII XmaCI XmaI