Restriction map for
pF1KB7772
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# NO CUT ENZYMES ( 116 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BlnI BpuAI BsaAI BsaWI
BseAI BseRI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstZ17I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MseI MslI MspA1I
MunI NarI NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 1138 GACGTC GACGT^C
AccII 39 CGCG CG^CG
AcsI 18 GAATTC [GA]^AATT[CT]
ApoI 18 GAATTC [GA]^AATT[CT]
Asp700I 776 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1357 GTGCTC G[AT]GC[AT]^C
AvaI 142 CCCGAG C^[CT]CG[GA]G
BalI 1223 TGGCCA TGG^CCA
BamHI 3 GGATCC G^GATCC
BanI 673 GGTGCC G^G[CT][GA]CC
BcgI 197 CGATCCTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 437 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 437 GCTCAGC GC^T[ACGT]AGC
BsaBI 1405 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 967 GGTCTC GGTCTC(1/5)
BsiEI 227 CGGCCG CG[GA][CT]^CG
BsiHKAI 1357 GTGCTC G[AT]GC[AT]^C
BsmAI 968 GTCTC GTCTC(1/5)
BsoBI 142 CCCGAG C^[CT]CG[GA]G
BspHI 1145 TCATGA T^CATGA
BstAPI 719 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 64 TTCGAA TT^CGAA
BstEII 999 GGTTACC G^GT[ACGT]ACC
BstPI 999 GGTTACC G^GT[ACGT]ACC
BstUI 39 CGCG CG^CG
CelII 437 GCTCAGC GC^T[ACGT]AGC
EagI 227 CGGCCG C^GGCCG
EclXI 227 CGGCCG C^GGCCG
Eco52I 227 CGGCCG C^GGCCG
EcoNI 544 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 999 GGTTACC G^GT[ACGT]ACC
EcoRI 18 GAATTC G^AATTC
HgaI 1232 GCGTC GACGC(5/10)
MaeII 1139 ACGT A^CGT
MamI 1405 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1223 TGGCCA TGG^CCA
MscI 1223 TGGCCA TGG^CCA
MvnI 39 CGCG CG^CG
NaeI 225 GCCGGC GCC^GGC
NcoI 590 CCATGG C^CATGG
NdeI 314 CATATG CA^TATG
NgoMIV 225 GCCGGC G^CCGGC
NspV 64 TTCGAA TT^CGAA
PflMI 1089 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 267 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1145 TCATGA T^CATGA
SfcI 1020 CTACAG C^T[GA][CT]AG
SfuI 64 TTCGAA TT^CGAA
SmlI 819 CTTGAG C^T[CT][GA]AG
TfiI 248 GAATC G^A[AT]TC
Tsp509I 19 AATT ^AATT
Van91I 1089 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 776 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 1138 GACGTC G[GA]^CG[CT]C
1231 GGCGTC
AocI 544 CCTGAGG CC^T[ACGT]AGG
1399 CCTCAGG
ApaI 234 GGGCCC GGGCC^C
1051 GGGCCC
BsaHI 1138 GACGTC G[GA]^CG[CT]C
1231 GGCGTC
BsgI 90 CTGCAC GTGCAG(16/14)
478 CTGCAC
BsmI 294 GAATGC GAATGC(1/-1)
1394 GAATGC
BsrBI 84 CCGCTC CCGCTC(-3/-3)
558 GAGCGG
Bsu36I 544 CCTGAGG CC^T[ACGT]AGG
1399 CCTCAGG
CfoI 186 GCGC GCG^C
347 GCGC
DsaI 590 CCATGG C^C[GA][CT]GG
1226 CCACGG
EarI 939 CTCTTC CTCTTC(1/4)
1184 CTCTTC
Eco81I 544 CCTGAGG CC^T[ACGT]AGG
1399 CCTCAGG
EcoT14I 590 CCATGG C^C[AT][AT]GG
1337 CCAAGG
HaeII 185 GGCGCT [GA]GCGC^[CT]
346 AGCGCC
HhaI 186 GCGC GCG^C
347 GCGC
Hin1I 1138 GACGTC G[GA]^CG[CT]C
1231 GGCGTC
HinP1I 186 GCGC G^CGC
347 GCGC
Ksp632I 939 CTCTTC CTCTTC(1/4)
1184 CTCTTC
SfaNI 534 GATGC GCATC(5/9)
990 GCATC
StyI 590 CCATGG C^C[AT][AT]GG
1337 CCAAGG
TspRI 795 CAGTG CA[GC]TG[ACGT][ACGT]^
1461 CAGTG
XbaI 909 TCTAGA T^CTAGA
1407 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BcnI BfaI
BglI BglII BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr10I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI TaqI TseI Tsp45I
TthHB8I XhoII