Restriction map for
pF1KE0341
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV EcoT14I FbaI FseI
FspI HpaI MamI MfeI MluI
MroI MunI NaeI NcoI NgoMIV
NotI NruI NspI NspV PacI
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RcaI RsrII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 1525 GACGTC GACGT^C
Acc65I 1853 GGTACC G^GTACC
AgeI 1541 ACCGGT A^CCGGT
AlwNI 1423 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 69 AGCGCT AGC^GCT
Asp700I 900 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1853 GGTACC G^GTACC
BbeI 1946 GGCGCC GGCGC^C
BciVI 801 GGATAC GTATCC(6/5)
BsaI 770 GAGACC GGTCTC(1/5)
BsiEI 1721 CGATCG CG[GA][CT]^CG
BsmI 1075 GCATTC GAATGC(1/-1)
BssHII 150 GCGCGC G^CGCGC
BssSI 1873 CTCGTG CACGAG(-5/-1)
BstYI 852 GGATCT [GA]^GATC[CT]
DrdI 1519 GACAAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 69 AGCGCT AGC^GCT
EcoRI 1383 GAATTC G^AATTC
EcoT22I 1073 ATGCAT ATGCA^T
HincII 568 GTTGAC GT[CT]^[GA]AC
HindII 568 GTTGAC GT[CT]^[GA]AC
HindIII 1782 AAGCTT A^AGCTT
KasI 1946 GGCGCC G^GCGCC
KpnI 1853 GGTACC GGTAC^C
MflI 852 GGATCT [GA]^GATC[CT]
NarI 1946 GGCGCC GG^CGCC
NdeI 718 CATATG CA^TATG
NheI 1105 GCTAGC G^CTAGC
NsiI 1073 ATGCAT ATGCA^T
PaeR7I 835 CTCGAG C^TCGAG
PflMI 1158 CCACTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1541 ACCGGT A^CCGGT
PpuMI 30 GGGTCCC [GA]G^G[AT]CC[CT]
PvuI 1721 CGATCG CGAT^CG
SexAI 563 ACCAGGT A^CC[AT]GGT
SfoI 1946 GGCGCC GGC^GCC
StuI 467 AGGCCT AGG^CCT
Van91I 1158 CCACTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 835 CTCGAG C^TCGAG
XhoII 852 GGATCT [GA]^GATC[CT]
XmnI 900 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 651 GATCC GGATC(4/5)
852 GGATC
AocI 622 CCTGAGG CC^T[ACGT]AGG
1857 CCTGAGG
ApaI 115 GGGCCC GGGCC^C
182 GGGCCC
BbsI 1693 GAAGAC GAAGAC(2/6)
1528 GTCTTC
BfaI 1106 CTAG C^TAG
1990 CTAG
BlpI 306 GCTCAGC GC^T[ACGT]AGC
723 GCTCAGC
Bpu1102I 306 GCTCAGC GC^T[ACGT]AGC
723 GCTCAGC
BpuAI 1693 GAAGAC GAAGAC(2/6)
1528 GTCTTC
BsaWI 1172 ACCGGA [AT]^CCGG[AT]
1541 ACCGGT
BseRI 1408 GAGGAG GAGGAG(10/8)
2044 GAGGAG
BsrBI 729 CCGCTC CCGCTC(-3/-3)
178 GAGCGG
BsrFI 1229 ACCGGC [GA]^CCGG[CT]
1541 ACCGGT
BstXI 759 CCACTACGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1965 CCAGCAGCTTGG
Bsu36I 622 CCTGAGG CC^T[ACGT]AGG
1857 CCTGAGG
CelII 306 GCTCAGC GC^T[ACGT]AGC
723 GCTCAGC
Cfr10I 1229 ACCGGC [GA]^CCGG[CT]
1541 ACCGGT
EarI 328 CTCTTC CTCTTC(1/4)
1474 CTCTTC
Eco81I 622 CCTGAGG CC^T[ACGT]AGG
1857 CCTGAGG
EcoNI 951 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1371 CCTGTTGAAGG
Ksp632I 328 CTCTTC CTCTTC(1/4)
1474 CTCTTC
MaeI 1106 CTAG C^TAG
1990 CTAG
MseI 1061 TTAA T^TAA
1702 TTAA
PstI 127 CTGCAG CTGCA^G
145 CTGCAG
PvuII 1291 CAGCTG CAG^CTG
1435 CAGCTG
SacI 1276 GAGCTC GAGCT^C
2047 GAGCTC
SapI 327 GCTCTTC GCTCTTC(1/4)
1473 GCTCTTC
SmaI 36 CCCGGG CCC^GGG
734 CCCGGG
SmlI 814 CTCAAG C^T[CT][GA]AG
835 CTCGAG
Tru9I 1061 TTAA T^TAA
1702 TTAA
XcmI 1154 CCAGCCACTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1418 CCATACAGATGCTGG
XmaCI 36 CCCGGG C^CCGGG
734 CCCGGG
XmaI 36 CCCGGG C^CCGGG
734 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I