Restriction map for
pF1KB6891
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AvaII AvrII BamHI
BanI BbeI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstF5I BstYI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoT14I
EcoT22I FbaI FokI FseI HapII
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeI MamI MfeI MflI MluI
MroI MseI MspA1I MspI MunI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsaI
RsrII SacII SalI SapI Sau96I
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tru9I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AspHI 82 GAGCTC G[AT]GC[AT]^C
AspI 6 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 467 TGCGCA TGC^GCA
BalI 362 TGGCCA TGG^CCA
BanII 82 GAGCTC G[GA]GC[CT]^C
BbrPI 454 CACGTG CAC^GTG
BbvI 216 GCTGC GCAGC(8/12)
BmyI 82 GAGCTC G[AGT]GC[ACT]^C
BpmI 79 CTGGAG CTGGAG(16/14)
BsaAI 454 CACGTG [CT]AC^GT[GA]
BseRI 184 GAGGAG GAGGAG(10/8)
BsgI 352 CTGCAC GTGCAG(16/14)
BsiEI 5 CGACCG CG[GA][CT]^CG
BsiHKAI 82 GAGCTC G[AT]GC[AT]^C
BsiYI 484 CCCGAATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 484 CCCGAATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 294 GAATGC GAATGC(1/-1)
Bsp1286I 82 GAGCTC G[AGT]GC[ACT]^C
BspHI 180 TCATGA T^CATGA
BsrBI 285 GAGCGG CCGCTC(-3/-3)
BsrDI 298 GCAATG GCAATG(2/0)
BstEII 448 GGTGACC G^GT[ACGT]ACC
BstPI 448 GGTGACC G^GT[ACGT]ACC
BstXI 225 CCAACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 287 GCGGGC GC[ACGT]^[ACGT]GC
DpnII 72 GATC ^GATC
DrdI 3 GACGACCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 448 GGTGACC G^GT[ACGT]ACC
EcoRV 253 GATATC GAT^ATC
FauI 287 GCGGG CCCGC(4/6)
FspI 467 TGCGCA TGC^GCA
HaeII 480 AGCGCC [GA]GCGC^[CT]
HgaI 10 GCGTC GACGC(5/10)
MboI 72 GATC ^GATC
MluNI 362 TGGCCA TGG^CCA
MscI 362 TGGCCA TGG^CCA
MslI 385 CATTTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 72 GATC ^GATC
NspI 195 ACATGC [GA]CATG^[CT]
PflFI 6 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 46 GACTC GAGTC(4/5)
PmaCI 454 CACGTG CAC^GTG
PmlI 454 CACGTG CAC^GTG
PstI 217 CTGCAG CTGCA^G
RcaI 180 TCATGA T^CATGA
SacI 82 GAGCTC GAGCT^C
Sau3AI 72 GATC ^GATC
SfcI 217 CTGCAG C^T[GA][CT]AG
TseI 216 GCTGC G^C[AT]GC
Tsp509I 443 AATT ^AATT
Tth111I 6 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 434 CCATCAAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AccII 9 CGCG CG^CG
130 CGCG
AluI 83 AGCT AG^CT
215 AGCT
BsaJI 524 CCCTGG C^C[ACGT][ACGT]GG
525 CCTGGG
BsmAI 403 GAGAC GTCTC(1/5)
530 GAGAC
BspMI 21 GCAGGT ACCTGC(4/8)
423 GCAGGT
BstUI 9 CGCG CG^CG
130 CGCG
DdeI 48 CTCAG C^T[ACGT]AG
200 CTGAG
EaeI 234 TGGCCG [CT]^GGCC[GA]
362 TGGCCA
Fnu4HI 55 GCCGC GC^[ACGT]GC
216 GCTGC
ItaI 55 GCCGC GC^[ACGT]GC
216 GCTGC
MaeII 98 ACGT A^CGT
455 ACGT
MvnI 9 CGCG CG^CG
130 CGCG
TspRI 366 CACTG CA[GC]TG[ACGT][ACGT]^
534 CAGTG
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
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AciI BsrI BssKI BstNI CfoI
EcoRII HaeIII HhaI HinP1I HinfI
HphI MaeIII MboII MnlI MvaI
NlaIII ScrFI TfiI Tsp45I