Restriction map for
pF1KE0335
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII AccII AclI AflII AgeI
AocI AscI AseI AsnI Asp700I
AspI AvaI AviII AvrII BbeI
BbvCI BcgI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FseI FspI
HincII HindII HpaI KasI KspI
MamI MluI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SmlI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1895 GGTACC G^GTACC
AccI 421 GTAGAC GT^[AC][GT]AC
AccIII 294 TCCGGA T^CCGGA
AcyI 92 GGCGTC G[GA]^CG[CT]C
AhdI 424 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 964 GTGCAC G^TGCAC
Aor51HI 2015 AGCGCT AGC^GCT
ApaI 921 GGGCCC GGGCC^C
ApaLI 964 GTGCAC G^TGCAC
Asp718I 1895 GGTACC G^GTACC
AspEI 424 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 680 GGATCC G^GATCC
BbrPI 2062 CACGTG CAC^GTG
BclI 2522 TGATCA T^GATCA
BcnI 2397 CCGGG CC^[GC]GG
BfaI 1885 CTAG C^TAG
BsaHI 92 GGCGTC G[GA]^CG[CT]C
BsaWI 294 TCCGGA [AT]^CCGG[AT]
BseAI 294 TCCGGA T^CCGGA
BspEI 294 TCCGGA T^CCGGA
BspHI 2081 TCATGA T^CATGA
BsrDI 2712 CATTGC GCAATG(2/0)
BstXI 1181 CCATGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2016 GCGC GCG^C
DraII 1011 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 2722 GACTGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1926 CCGTGG C^C[GA][CT]GG
Eam1105I 424 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2015 AGCGCT AGC^GCT
EcoO109I 1011 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 610 GATATC GAT^ATC
FauI 1853 GCGGG CCCGC(4/6)
FbaI 2522 TGATCA T^GATCA
HaeII 2015 AGCGCT [GA]GCGC^[CT]
HgaI 93 GCGTC GACGC(5/10)
HhaI 2016 GCGC GCG^C
Hin1I 92 GGCGTC G[GA]^CG[CT]C
HinP1I 2016 GCGC G^CGC
HindIII 1291 AAGCTT A^AGCTT
KpnI 1895 GGTACC GGTAC^C
MaeI 1885 CTAG C^TAG
MfeI 1599 CAATTG C^AATTG
MroI 294 TCCGGA T^CCGGA
MspA1I 1104 CCGCTG C[AC]G^C[GT]G
MunI 1599 CAATTG C^AATTG
NciI 2397 CCGGG CC^[GC]GG
PmaCI 2062 CACGTG CAC^GTG
PmlI 2062 CACGTG CAC^GTG
PpuMI 1011 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 2081 TCATGA T^CATGA
SacI 1615 GAGCTC GAGCT^C
ScaI 1040 AGTACT AGT^ACT
SexAI 169 ACCAGGT A^CC[AT]GGT
SnaBI 2661 TACGTA TAC^GTA
StuI 2086 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflIII 1708 ACACGT A^C[GA][CT]GT
2061 ACACGT
AvaII 1012 GGACC G^G[AT]CC
2730 GGTCC
BanI 1741 GGTGCC G^G[CT][GA]CC
1895 GGTACC
BciVI 763 GGATAC GTATCC(6/5)
1090 GGATAC
BpmI 1904 CTGGAG CTGGAG(16/14)
2692 CTGGAG
BsaAI 2062 CACGTG [CT]AC^GT[GA]
2661 TACGTA
BsmFI 347 GGGAC GGGAC(10/14)
1229 GGGAC
BsmI 855 GAATGC GAATGC(1/-1)
1581 GAATGC
Cac8I 311 GCAAGC GC[ACGT]^[ACGT]GC
891 GCCTGC
EcoT14I 1451 CCAAGG C^C[AT][AT]GG
2294 CCTTGG
HapII 295 CCGG C^CGG
2397 CCGG
HpaII 295 CCGG C^CGG
2397 CCGG
MslI 670 CATCTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
957 CACTCATGTG
MspI 295 CCGG C^CGG
2397 CCGG
NspI 690 ACATGC [GA]CATG^[CT]
2144 ACATGC
PstI 893 CTGCAG CTGCA^G
1655 CTGCAG
StyI 1451 CCAAGG C^C[AT][AT]GG
2294 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI BalI BanII
BbsI BbvI BglII BmyI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EaeI EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII