Restriction map for
pF1KE0184
##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BspDI BspLU11I
BspMI BsrDI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI Ksp632I
MamI MfeI MluI MluNI MscI
MunI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflIII 344 ACACGT A^C[GA][CT]GT
Alw44I 697 GTGCAC G^TGCAC
ApaLI 697 GTGCAC G^TGCAC
AspHI 697 GTGCAC G[AT]GC[AT]^C
AvaI 119 CTCGAG C^[CT]CG[GA]G
BanII 617 GGGCTC G[GA]GC[CT]^C
BbrPI 345 CACGTG CAC^GTG
BglII 115 AGATCT A^GATCT
BsaAI 345 CACGTG [CT]AC^GT[GA]
BsiHKAI 697 GTGCAC G[AT]GC[AT]^C
BsmFI 334 GGGAC GGGAC(10/14)
BsoBI 119 CTCGAG C^[CT]CG[GA]G
Bsp1407I 689 TGTACA T^GTACA
BsrBI 29 GAGCGG CCGCTC(-3/-3)
BsrFI 40 GCCGGC [GA]^CCGG[CT]
BsrGI 689 TGTACA T^GTACA
BsrI 614 ACTGG ACTGG(1/-1)
BssHII 18 GCGCGC G^CGCGC
BssSI 620 CTCGTG CACGAG(-5/-1)
BstF5I 642 GGATG GGATG(2/0)
Cfr10I 40 GCCGGC [GA]^CCGG[CT]
DrdI 364 GACCAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 24 CCGCGG C^C[GA][CT]GG
FokI 642 GGATG GGATG(9/13)
KspI 24 CCGCGG CCGC^GG
MaeII 346 ACGT A^CGT
MseI 242 TTAA T^TAA
MslI 760 CACAAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 40 GCCGGC GCC^GGC
NgoMIV 40 GCCGGC G^CCGGC
NspI 476 ACATGC [GA]CATG^[CT]
PaeR7I 119 CTCGAG C^TCGAG
PmaCI 345 CACGTG CAC^GTG
PmlI 345 CACGTG CAC^GTG
SacII 24 CCGCGG CCGC^GG
SfaNI 175 GATGC GCATC(5/9)
SmlI 119 CTCGAG C^T[CT][GA]AG
SspBI 689 TGTACA T^GTACA
TaqI 120 TCGA T^CGA
Tru9I 242 TTAA T^TAA
Tsp45I 473 GTGAC ^GT[GC]AC
TspRI 836 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 120 TCGA T^CGA
XhoI 119 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 323 GTAC GT^AC
690 GTAC
AlwNI 499 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
516 CAGTTTCTG
AvaII 363 GGACC G^G[AT]CC
547 GGACC
BfaI 193 CTAG C^TAG
256 CTAG
BmyI 617 GGGCTC G[AGT]GC[ACT]^C
697 GTGCAC
BsmI 250 GAATGC GAATGC(1/-1)
747 GAATGC
Bsp1286I 617 GGGCTC G[AGT]GC[ACT]^C
697 GTGCAC
BspHI 419 TCATGA T^CATGA
827 TCATGA
FauI 23 CCCGC CCCGC(4/6)
57 GCGGG
MaeI 193 CTAG C^TAG
256 CTAG
MaeIII 473 GTGAC ^GT[ACGT]AC
647 GTAAC
PstI 52 CTGCAG CTGCA^G
69 CTGCAG
PvuII 129 CAGCTG CAG^CTG
685 CAGCTG
RcaI 419 TCATGA T^CATGA
827 TCATGA
RsaI 323 GTAC GT^AC
690 GTAC
SfcI 52 CTGCAG C^T[GA][CT]AG
69 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AccIII AciI AcsI AluI
AlwI ApoI BbsI BbvI BpmI
BpuAI BsaJI BsaWI BseAI BsiYI
BslI BspEI BssKI BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboI MboII MflI MnlI MroI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TfiI TseI Tsp509I XhoII