Restriction map for
pF1KF0316
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BclI BcnI
BglI BglII BlnI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI StyI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AseI 1768 ATTAAT AT^TAAT
AsnI 1768 ATTAAT AT^TAAT
BalI 167 TGGCCA TGG^CCA
BanII 246 GGGCTC G[GA]GC[CT]^C
BbsI 1141 GTCTTC GAAGAC(2/6)
BbvCI 532 GCTGAGG CCTCAGC(-5/-2)
BlpI 1267 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1267 GCTGAGC GC^T[ACGT]AGC
BpuAI 1141 GTCTTC GAAGAC(2/6)
BsaAI 1913 CACGTA [CT]AC^GT[GA]
BsmAI 1220 GAGAC GTCTC(1/5)
BsrBI 1516 GAGCGG CCGCTC(-3/-3)
BsrFI 755 ACCGGC [GA]^CCGG[CT]
BssSI 381 CTCGTG CACGAG(-5/-1)
BstXI 59 CCATCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1267 GCTGAGC GC^T[ACGT]AGC
CfoI 228 GCGC GCG^C
Cfr10I 755 ACCGGC [GA]^CCGG[CT]
DraI 780 TTTAAA TTT^AAA
DraIII 472 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 167 TGGCCA [CT]^GGCC[GA]
FauI 1518 GCGGG CCCGC(4/6)
HapII 756 CCGG C^CGG
HhaI 228 GCGC GCG^C
HinP1I 228 GCGC G^CGC
HpaII 756 CCGG C^CGG
MluNI 167 TGGCCA TGG^CCA
MscI 167 TGGCCA TGG^CCA
MspI 756 CCGG C^CGG
NdeI 787 CATATG CA^TATG
PpuMI 1418 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1768 ATTAAT AT^TAAT
SapI 97 GAAGAGC GCTCTTC(1/4)
SexAI 36 ACCAGGT A^CC[AT]GGT
SphI 1110 GCATGC GCATG^C
SwaI 779 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 1273 CTTAAG C^TTAAG
1723 CTTAAG
AlwI 1827 GATCC GGATC(4/5)
386 GGATC
Asp700I 1660 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1729 GAAGAATTTC
AvaII 324 GGTCC G^G[AT]CC
1419 GGTCC
BfrI 1273 CTTAAG C^TTAAG
1723 CTTAAG
BsmI 648 GAATGC GAATGC(1/-1)
981 GAATGC
BspHI 62 TCATGA T^CATGA
206 TCATGA
BsrDI 1134 CATTGC GCAATG(2/0)
230 GCAATG
BsrI 907 ACTGG ACTGG(1/-1)
996 ACTGG
BstYI 386 GGATCT [GA]^GATC[CT]
1826 AGATCC
DpnII 387 GATC ^GATC
1827 GATC
DsaI 155 CCGTGG C^C[GA][CT]GG
251 CCACGG
EcoNI 885 CCTGAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1118 CCTGTGAGAGG
EcoRI 114 GAATTC G^AATTC
1177 GAATTC
HindIII 1189 AAGCTT A^AGCTT
1608 AAGCTT
MaeII 1106 ACGT A^CGT
1914 ACGT
MboI 387 GATC ^GATC
1827 GATC
MflI 386 GGATCT [GA]^GATC[CT]
1826 AGATCC
MslI 60 CATCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
276 CATCTGGATG
NdeII 387 GATC ^GATC
1827 GATC
NheI 486 GCTAGC G^CTAGC
1840 GCTAGC
NspI 698 ACATGC [GA]CATG^[CT]
1110 GCATGC
RcaI 62 TCATGA T^CATGA
206 TCATGA
Sau3AI 387 GATC ^GATC
1827 GATC
SspI 776 AATATT AAT^ATT
1256 AATATT
XcmI 74 CCATTTGTCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
565 CCAGACTTTATTTGG
XhoII 386 GGATCT [GA]^GATC[CT]
1826 AGATCC
XmnI 1660 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1729 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI BbvI BfaI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboII MnlI MseI
MspA1I MvaI MwoI NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I