################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeII MaeIII MamI MboI MfeI MflI MluI MroI MseI MslI MspA1I MunI MvnI NaeI NarI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AciI 168 CCGC CCGC(-3/-1) AfaI 267 GTAC GT^AC AlwNI 283 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 149 GAGCTC G[AT]GC[AT]^C AvaI 146 CCCGAG C^[CT]CG[GA]G AvaII 14 GGACC G^G[AT]CC BalI 59 TGGCCA TGG^CCA BcnI 171 CCCGG CC^[GC]GG BfaI 154 CTAG C^TAG BglI 325 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 72 CTCCAG CTGGAG(16/14) BsaWI 218 TCCGGT [AT]^CCGG[AT] BseRI 34 CTCCTC GAGGAG(10/8) BsiHKAI 149 GAGCTC G[AT]GC[AT]^C BsmFI 13 GGGAC GGGAC(10/14) BsoBI 146 CCCGAG C^[CT]CG[GA]G BsrI 210 ACTGG ACTGG(1/-1) DdeI 82 CTGAG C^T[ACGT]AG DsaI 62 CCATGG C^C[GA][CT]GG EaeI 59 TGGCCA [CT]^GGCC[GA] EarI 319 GAAGAG CTCTTC(1/4) EcoT14I 62 CCATGG C^C[AT][AT]GG Ksp632I 319 GAAGAG CTCTTC(1/4) MaeI 154 CTAG C^TAG MluNI 59 TGGCCA TGG^CCA MscI 59 TGGCCA TGG^CCA NciI 171 CCCGG CC^[GC]GG NcoI 62 CCATGG C^CATGG NspI 41 GCATGC [GA]CATG^[CT] PflMI 282 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 267 GTAC GT^AC SacI 149 GAGCTC GAGCT^C SfiI 324 GGCCAAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 41 GCATGC GCATG^C StyI 62 CCATGG C^C[AT][AT]GG TaqI 118 TCGA T^CGA TfiI 245 GAATC G^A[AT]TC TthHB8I 118 TCGA T^CGA Van91I 282 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BanII 84 GAGCCC G[GA]GC[CT]^C 149 GAGCTC BmyI 84 GAGCCC G[AGT]GC[ACT]^C 149 GAGCTC Bsp1286I 84 GAGCCC G[AGT]GC[ACT]^C 149 GAGCTC BstF5I 309 CATCC GGATG(2/0) 204 GGATG DraII 284 AGGCCCT [GA]G^G[ACGT]CC[CT] 332 AGGCCCC EcoO109I 284 AGGCCCT [GA]G^G[ACGT]CC[CT] 332 AGGCCCC FokI 309 CATCC GGATG(9/13) 204 GGATG HapII 172 CCGG C^CGG 219 CCGG HpaII 172 CCGG C^CGG 219 CCGG MspI 172 CCGG C^CGG 219 CCGG NlaIII 42 CATG CATG^ 63 CATG PleI 101 GAGTC GAGTC(4/5) 115 GAGTC PstI 21 CTGCAG CTGCA^G 139 CTGCAG SfcI 21 CTGCAG C^T[GA][CT]AG 139 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AluI BbvI BsaJI BsiYI BslI BssKI BstNI Cac8I Cfr13I EcoRII Fnu4HI HaeIII HinfI ItaI MboII MnlI MvaI MwoI NlaIV Sau96I ScrFI TseI