Restriction map for pF1KB6769
##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        KspI        MaeII       MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MroI        MseI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tsp45I      Tsp509I     TspRI       
Tth111I     XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AciI          168  CCGC                 CCGC(-3/-1)
AfaI          267  GTAC                 GT^AC
AlwNI         283  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         149  GAGCTC               G[AT]GC[AT]^C
AvaI          146  CCCGAG               C^[CT]CG[GA]G
AvaII          14  GGACC                G^G[AT]CC
BalI           59  TGGCCA               TGG^CCA
BcnI          171  CCCGG                CC^[GC]GG
BfaI          154  CTAG                 C^TAG
BglI          325  GCCAAAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI           72  CTCCAG               CTGGAG(16/14)
BsaWI         218  TCCGGT               [AT]^CCGG[AT]
BseRI          34  CTCCTC               GAGGAG(10/8)
BsiHKAI       149  GAGCTC               G[AT]GC[AT]^C
BsmFI          13  GGGAC                GGGAC(10/14)
BsoBI         146  CCCGAG               C^[CT]CG[GA]G
BsrI          210  ACTGG                ACTGG(1/-1)
DdeI           82  CTGAG                C^T[ACGT]AG
DsaI           62  CCATGG               C^C[GA][CT]GG
EaeI           59  TGGCCA               [CT]^GGCC[GA]
EarI          319  GAAGAG               CTCTTC(1/4)
EcoT14I        62  CCATGG               C^C[AT][AT]GG
Ksp632I       319  GAAGAG               CTCTTC(1/4)
MaeI          154  CTAG                 C^TAG
MluNI          59  TGGCCA               TGG^CCA
MscI           59  TGGCCA               TGG^CCA
NciI          171  CCCGG                CC^[GC]GG
NcoI           62  CCATGG               C^CATGG
NspI           41  GCATGC               [GA]CATG^[CT]
PflMI         282  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI          267  GTAC                 GT^AC
SacI          149  GAGCTC               GAGCT^C
SfiI          324  GGCCAAAGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI           41  GCATGC               GCATG^C
StyI           62  CCATGG               C^C[AT][AT]GG
TaqI          118  TCGA                 T^CGA
TfiI          245  GAATC                G^A[AT]TC
TthHB8I       118  TCGA                 T^CGA
Van91I        282  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BanII          84  GAGCCC               G[GA]GC[CT]^C
              149  GAGCTC              
BmyI           84  GAGCCC               G[AGT]GC[ACT]^C
              149  GAGCTC              
Bsp1286I       84  GAGCCC               G[AGT]GC[ACT]^C
              149  GAGCTC              
BstF5I        309  CATCC                GGATG(2/0)
              204  GGATG               
DraII         284  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              332  AGGCCCC             
EcoO109I      284  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              332  AGGCCCC             
FokI          309  CATCC                GGATG(9/13)
              204  GGATG               
HapII         172  CCGG                 C^CGG
              219  CCGG                
HpaII         172  CCGG                 C^CGG
              219  CCGG                
MspI          172  CCGG                 C^CGG
              219  CCGG                
NlaIII         42  CATG                 CATG^
               63  CATG                
PleI          101  GAGTC                GAGTC(4/5)
              115  GAGTC               
PstI           21  CTGCAG               CTGCA^G
              139  CTGCAG              
SfcI           21  CTGCAG               C^T[GA][CT]AG
              139  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsiYI       BslI        
BssKI       BstNI       Cac8I       Cfr13I      EcoRII      
Fnu4HI      HaeIII      HinfI       ItaI        MboII       
MnlI        MvaI        MwoI        NlaIV       Sau96I      
ScrFI       TseI        
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