Restriction map for
pF1KB6507
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BalI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaI BsgI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI KspI
MaeI MfeI MluI MluNI MscI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 235 GTCTAC GT^[AC][GT]AC
AflIII 985 ACGTGT A^C[GA][CT]GT
AspI 706 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 176 TGCGCA TGC^GCA
BamHI 647 GGATCC G^GATCC
BanI 190 GGCGCC G^G[CT][GA]CC
BanII 115 GAGCTC G[GA]GC[CT]^C
BbeI 190 GGCGCC GGCGC^C
BbrPI 984 CACGTG CAC^GTG
BbvCI 693 CCTCAGC CCTCAGC(-5/-2)
BsaAI 984 CACGTG [CT]AC^GT[GA]
BsaBI 174 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 283 GAGAC GTCTC(1/5)
BsmBI 283 GAGACG CGTCTC(1/5)
BsmI 427 GAATGC GAATGC(1/-1)
BstAPI 528 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 360 GGTGACC G^GT[ACGT]ACC
BstPI 360 GGTGACC G^GT[ACGT]ACC
DraIII 859 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3 GACTCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1025 CGGCCG [CT]^GGCC[GA]
EagI 1025 CGGCCG C^GGCCG
EclXI 1025 CGGCCG C^GGCCG
Eco52I 1025 CGGCCG C^GGCCG
EcoO65I 360 GGTGACC G^GT[ACGT]ACC
FspI 176 TGCGCA TGC^GCA
HaeII 190 GGCGCC [GA]GCGC^[CT]
HincII 490 GTTGAC GT[CT]^[GA]AC
HindII 490 GTTGAC GT[CT]^[GA]AC
KasI 190 GGCGCC G^GCGCC
MamI 174 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 252 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 193 GCCGGC GCC^GGC
NarI 190 GGCGCC GG^CGCC
NcoI 278 CCATGG C^CATGG
NgoMIV 193 GCCGGC G^CCGGC
PflFI 706 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 708 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 984 CACGTG CAC^GTG
PmlI 984 CACGTG CAC^GTG
PstI 1045 CTGCAG CTGCA^G
PvuII 82 CAGCTG CAG^CTG
SacI 115 GAGCTC GAGCT^C
SapI 153 GAAGAGC GCTCTTC(1/4)
ScaI 473 AGTACT AGT^ACT
SfcI 1045 CTGCAG C^T[GA][CT]AG
SfoI 190 GGCGCC GGC^GCC
SmaI 414 CCCGGG CCC^GGG
Tth111I 706 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 708 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 414 CCCGGG C^CCGGG
XmaI 414 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 602 TCCGGA T^CCGGA
764 TCCGGA
Asp700I 243 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
930 GAAGATGTTC
AspHI 115 GAGCTC G[AT]GC[AT]^C
156 GAGCAC
AvaI 414 CCCGGG C^[CT]CG[GA]G
877 CCCGAG
BglI 256 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
904 GCCCAGGCGGC
BseAI 602 TCCGGA T^CCGGA
764 TCCGGA
BsiHKAI 115 GAGCTC G[AT]GC[AT]^C
156 GAGCAC
BsoBI 414 CCCGGG C^[CT]CG[GA]G
877 CCCGAG
BspEI 602 TCCGGA T^CCGGA
764 TCCGGA
BspMI 515 ACCTGC ACCTGC(4/8)
830 ACCTGC
BsrBI 147 GAGCGG CCGCTC(-3/-3)
634 GAGCGG
BsrFI 193 GCCGGC [GA]^CCGG[CT]
559 ACCGGC
Cfr10I 193 GCCGGC [GA]^CCGG[CT]
559 ACCGGC
DdeI 31 CTGAG C^T[ACGT]AG
694 CTCAG
DraII 410 AGGACCC [GA]G^G[ACGT]CC[CT]
827 AGGACCT
DsaI 186 CCACGG C^C[GA][CT]GG
278 CCATGG
EarI 811 CTCTTC CTCTTC(1/4)
153 GAAGAG
EcoNI 402 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
831 CCTGCTGGAGG
EcoO109I 410 AGGACCC [GA]G^G[ACGT]CC[CT]
827 AGGACCT
EcoT14I 278 CCATGG C^C[AT][AT]GG
506 CCTTGG
Ksp632I 811 CTCTTC CTCTTC(1/4)
153 GAAGAG
MaeIII 361 GTGAC ^GT[ACGT]AC
667 GTGAC
MroI 602 TCCGGA T^CCGGA
764 TCCGGA
PpuMI 410 AGGACCC [GA]G^G[AT]CC[CT]
827 AGGACCT
SexAI 860 ACCTGGT A^CC[AT]GGT
1070 ACCTGGT
StyI 278 CCATGG C^C[AT][AT]GG
506 CCTTGG
Tsp45I 361 GTGAC ^GT[GC]AC
667 GTGAC
TspRI 387 CAGTG CA[GC]TG[ACGT][ACGT]^
652 CACTG
XmnI 243 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
930 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsaWI BseRI
BsiEI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DpnII EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
TseI TthHB8I XhoII