Restriction map for
pF1KE0312
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI BstBI BstEII BstPI BstXI
BstYI CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KspI
MaeI MamI MfeI MflI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I XbaI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 1361 GGTACC G^GTACC
AccI 753 GTATAC GT^[AC][GT]AC
AccII 118 CGCG CG^CG
Aor51HI 912 AGCGCT AGC^GCT
ApaI 164 GGGCCC GGGCC^C
Asp718I 1361 GGTACC G^GTACC
AvaII 944 GGACC G^G[AT]CC
BalI 1329 TGGCCA TGG^CCA
BciVI 56 GTATCC GTATCC(6/5)
BpmI 661 CTCCAG CTGGAG(16/14)
Bst1107I 753 GTATAC GTA^TAC
BstAPI 299 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 118 CGCG CG^CG
BstZ17I 753 GTATAC GTA^TAC
DsaI 1365 CCATGG C^C[GA][CT]GG
Eco47III 912 AGCGCT AGC^GCT
EcoNI 1526 CCTTTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KpnI 1361 GGTACC GGTAC^C
MaeII 926 ACGT A^CGT
MluNI 1329 TGGCCA TGG^CCA
MscI 1329 TGGCCA TGG^CCA
MvnI 118 CGCG CG^CG
NcoI 1365 CCATGG C^CATGG
NspI 356 GCATGT [GA]CATG^[CT]
PaeR7I 272 CTCGAG C^TCGAG
PflMI 1278 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 652 CAGCTG CAG^CTG
SacI 976 GAGCTC GAGCT^C
SexAI 41 ACCTGGT A^CC[AT]GGT
SgrAI 370 CGCCGGTG C[GA]^CCGG[CT]G
TfiI 382 GATTC G^A[AT]TC
Tsp509I 198 AATT ^AATT
Van91I 1278 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 572 CCAGAGGGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 272 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwI 615 GATCC GGATC(4/5)
641 GGATC
AocI 826 CCTGAGG CC^T[ACGT]AGG
1309 CCTGAGG
AspHI 976 GAGCTC G[AT]GC[AT]^C
1296 GTGCTC
AspI 1135 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1467 GACGGAGTC
BbvCI 217 GCTGAGG CCTCAGC(-5/-2)
1270 GCTGAGG
BglI 1202 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1383 GCCATTTGGGC
BsiHKAI 976 GAGCTC G[AT]GC[AT]^C
1296 GTGCTC
BsmFI 391 GGGAC GGGAC(10/14)
586 GGGAC
BsrDI 1565 CATTGC GCAATG(2/0)
698 GCAATG
BsrFI 146 ACCGGC [GA]^CCGG[CT]
371 GCCGGT
Bsu36I 826 CCTGAGG CC^T[ACGT]AGG
1309 CCTGAGG
Cfr10I 146 ACCGGC [GA]^CCGG[CT]
371 GCCGGT
EaeI 245 CGGCCA [CT]^GGCC[GA]
1329 TGGCCA
Eco81I 826 CCTGAGG CC^T[ACGT]AGG
1309 CCTGAGG
HgaI 311 GCGTC GACGC(5/10)
1149 GCGTC
MaeIII 928 GTGAC ^GT[ACGT]AC
1473 GTCAC
MspA1I 215 CCGCTG C[AC]G^C[GT]G
652 CAGCTG
PflFI 1135 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1467 GACGGAGTC
SapI 112 GAAGAGC GCTCTTC(1/4)
742 GAAGAGC
TaqI 273 TCGA T^CGA
1196 TCGA
Tsp45I 928 GTGAC ^GT[GC]AC
1473 GTCAC
Tth111I 1135 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1467 GACGGAGTC
TthHB8I 273 TCGA T^CGA
1196 TCGA
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
BanI BanII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MboI MboII MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TseI TspRI