Restriction map for
pF1KE0002
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
Eam1105I Eco81I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1409 GTCGAC GT^[AC][GT]AC
AcsI 316 AAATTT [GA]^AATT[CT]
AflII 724 CTTAAG C^TTAAG
AlwI 903 GATCC GGATC(4/5)
Aor51HI 720 AGCGCT AGC^GCT
ApoI 316 AAATTT [GA]^AATT[CT]
BbeI 1278 GGCGCC GGCGC^C
BclI 230 TGATCA T^GATCA
BfaI 1589 CTAG C^TAG
BfrI 724 CTTAAG C^TTAAG
BglI 1566 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 968 AGATCT A^GATCT
BsaWI 1291 ACCGGA [AT]^CCGG[AT]
BsmBI 766 CGTCTC CGTCTC(1/5)
BsrBI 1129 GAGCGG CCGCTC(-3/-3)
BstYI 968 AGATCT [GA]^GATC[CT]
DrdI 945 GACGGCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1430 CCACGG C^C[GA][CT]GG
Eco47III 720 AGCGCT AGC^GCT
EcoNI 261 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 230 TGATCA T^GATCA
KasI 1278 GGCGCC G^GCGCC
MaeI 1589 CTAG C^TAG
MaeII 833 ACGT A^CGT
MflI 968 AGATCT [GA]^GATC[CT]
MslI 957 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1100 GCCGGC GCC^GGC
NarI 1278 GGCGCC GG^CGCC
NgoMIV 1100 GCCGGC G^CCGGC
NlaIII 1251 CATG CATG^
PpuMI 794 GGGTCCC [GA]G^G[AT]CC[CT]
SalI 1409 GTCGAC G^TCGAC
SapI 372 GCTCTTC GCTCTTC(1/4)
SbfI 990 CCTGCAGG CCTGCA^GG
SfiI 1565 GGCCTCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1278 GGCGCC GGC^GCC
Sse8387I 990 CCTGCAGG CCTGCA^GG
StuI 258 AGGCCT AGG^CCT
TfiI 460 GAATC G^A[AT]TC
Tsp509I 317 AATT ^AATT
XhoII 968 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BsaI 100 GAGACC GGTCTC(1/5)
895 GGTCTC
BstEII 81 GGTCACC G^GT[ACGT]ACC
1116 GGTGACC
BstPI 81 GGTCACC G^GT[ACGT]ACC
1116 GGTGACC
EarI 373 CTCTTC CTCTTC(1/4)
1090 GAAGAG
EcoO65I 81 GGTCACC G^GT[ACGT]ACC
1116 GGTGACC
HaeII 720 AGCGCT [GA]GCGC^[CT]
1278 GGCGCC
HincII 1351 GTCAAC GT[CT]^[GA]AC
1409 GTCGAC
HindII 1351 GTCAAC GT[CT]^[GA]AC
1409 GTCGAC
Ksp632I 373 CTCTTC CTCTTC(1/4)
1090 GAAGAG
MaeIII 82 GTCAC ^GT[ACGT]AC
1117 GTGAC
MseI 136 TTAA T^TAA
725 TTAA
SacI 1177 GAGCTC GAGCT^C
1363 GAGCTC
SmlI 724 CTTAAG C^T[CT][GA]AG
1366 CTCAAG
Tru9I 136 TTAA T^TAA
725 TTAA
Tsp45I 82 GTCAC ^GT[GC]AC
1117 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI TaqI TseI TspRI TthHB8I
XmaCI XmaI