Restriction map for
pF1KB7324
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AscI AseI
AsnI Asp700I Asp718I AspEI BalI
BcgI BclI BfrI BglII BsaBI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluNI MroI MscI MseI
MunI NdeI NheI NotI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfuI SmlI SnaBI SpeI SspBI
SspI SwaI Tru9I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 23 enzymes) #
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AcsI 2331 GAATTT [GA]^AATT[CT]
ApoI 2331 GAATTT [GA]^AATT[CT]
AviII 2789 TGCGCA TGC^GCA
AvrII 474 CCTAGG C^CTAGG
BamHI 2843 GGATCC G^GATCC
BciVI 1556 GTATCC GTATCC(6/5)
BlnI 474 CCTAGG C^CTAGG
BlpI 1416 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1416 GCTGAGC GC^T[ACGT]AGC
BsaI 1240 GAGACC GGTCTC(1/5)
BsmBI 309 CGTCTC CGTCTC(1/5)
BsmI 1981 GCATTC GAATGC(1/-1)
BssHII 2925 GCGCGC G^CGCGC
CelII 1416 GCTGAGC GC^T[ACGT]AGC
DrdI 111 GACACCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 2789 TGCGCA TGC^GCA
MluI 2225 ACGCGT A^CGCGT
NruI 1161 TCGCGA TCG^CGA
NspI 3112 GCATGC [GA]CATG^[CT]
SacI 2125 GAGCTC GAGCT^C
SgrAI 2370 CGCCGGCG C[GA]^CCGG[CT]G
SphI 3112 GCATGC GCATG^C
XcmI 2708 CCATCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AflIII 2225 ACGCGT A^C[GA][CT]GT
3092 ACGTGT
AocI 1426 CCTGAGG CC^T[ACGT]AGG
2108 CCTCAGG
Aor51HI 1626 AGCGCT AGC^GCT
3669 AGCGCT
AspI 3007 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3705 GACACAGTC
BbrPI 2961 CACGTG CAC^GTG
3349 CACGTG
BssSI 672 CACGAG CACGAG(-5/-1)
1087 CACGAG
BstYI 2163 GGATCT [GA]^GATC[CT]
2843 GGATCC
Bsu36I 1426 CCTGAGG CC^T[ACGT]AGG
2108 CCTCAGG
DpnII 2164 GATC ^GATC
2844 GATC
DraIII 2212 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
3297 CACCCCGTG
Eco47III 1626 AGCGCT AGC^GCT
3669 AGCGCT
Eco81I 1426 CCTGAGG CC^T[ACGT]AGG
2108 CCTCAGG
MboI 2164 GATC ^GATC
2844 GATC
MflI 2163 GGATCT [GA]^GATC[CT]
2843 GGATCC
NcoI 752 CCATGG C^CATGG
3593 CCATGG
NdeII 2164 GATC ^GATC
2844 GATC
PflFI 3007 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3705 GACACAGTC
PmaCI 2961 CACGTG CAC^GTG
3349 CACGTG
PmlI 2961 CACGTG CAC^GTG
3349 CACGTG
SapI 292 GCTCTTC GCTCTTC(1/4)
1515 GCTCTTC
Sau3AI 2164 GATC ^GATC
2844 GATC
SbfI 3255 CCTGCAGG CCTGCA^GG
3357 CCTGCAGG
Sse8387I 3255 CCTGCAGG CCTGCA^GG
3357 CCTGCAGG
TaqI 185 TCGA T^CGA
813 TCGA
Tth111I 3007 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3705 GACACAGTC
TthHB8I 185 TCGA T^CGA
813 TCGA
XhoII 2163 GGATCT [GA]^GATC[CT]
2843 GGATCC
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI StuI StyI TfiI
TseI Tsp45I Tsp509I TspRI Van91I
XmaCI XmaI