Restriction map for
pF1KB7347
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# NO CUT ENZYMES ( 69 enzymes) #
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AclI AflII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
BcgI BfrI BglII BlpI Bpu1102I
BsaBI BsiWI Bsp1407I BspDI BspHI
BsrDI BsrGI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DrdI
Eam1105I EcoRI EcoRV EcoT22I FseI
HindIII HpaI MamI MfeI MluI
MseI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 2182 GACGTC GACGT^C
Acc65I 1291 GGTACC G^GTACC
AccI 2761 GTCTAC GT^[AC][GT]AC
AcsI 1690 AAATTC [GA]^AATT[CT]
AflIII 2852 ACATGT A^C[GA][CT]GT
ApoI 1690 AAATTC [GA]^AATT[CT]
Asp700I 1686 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1291 GGTACC G^GTACC
AvrII 1179 CCTAGG C^CTAGG
BalI 2195 TGGCCA TGG^CCA
BamHI 1260 GGATCC G^GATCC
BbeI 3145 GGCGCC GGCGC^C
BbrPI 3055 CACGTG CAC^GTG
BbvCI 2584 GCTGAGG CCTCAGC(-5/-2)
BciVI 2339 GTATCC GTATCC(6/5)
BclI 725 TGATCA T^GATCA
BlnI 1179 CCTAGG C^CTAGG
BsaAI 3055 CACGTG [CT]AC^GT[GA]
BsiEI 3292 CGGCCG CG[GA][CT]^CG
BsmBI 1187 GAGACG CGTCTC(1/5)
BsmI 532 GCATTC GAATGC(1/-1)
BspLU11I 2852 ACATGT A^CATGT
BssHII 544 GCGCGC G^CGCGC
BstEII 558 GGTCACC G^GT[ACGT]ACC
BstPI 558 GGTCACC G^GT[ACGT]ACC
DraIII 739 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3292 CGGCCG C^GGCCG
EarI 1877 CTCTTC CTCTTC(1/4)
EclXI 3292 CGGCCG C^GGCCG
Eco52I 3292 CGGCCG C^GGCCG
EcoO65I 558 GGTCACC G^GT[ACGT]ACC
FbaI 725 TGATCA T^GATCA
HincII 3007 GTCAAC GT[CT]^[GA]AC
HindII 3007 GTCAAC GT[CT]^[GA]AC
KasI 3145 GGCGCC G^GCGCC
KpnI 1291 GGTACC GGTAC^C
Ksp632I 1877 CTCTTC CTCTTC(1/4)
MluNI 2195 TGGCCA TGG^CCA
MscI 2195 TGGCCA TGG^CCA
NarI 3145 GGCGCC GG^CGCC
NdeI 2899 CATATG CA^TATG
PmaCI 3055 CACGTG CAC^GTG
PmlI 3055 CACGTG CAC^GTG
SbfI 3246 CCTGCAGG CCTGCA^GG
SexAI 3104 ACCAGGT A^CC[AT]GGT
SfiI 1440 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3145 GGCGCC GGC^GCC
SphI 2891 GCATGC GCATG^C
Sse8387I 3246 CCTGCAGG CCTGCA^GG
Tsp509I 1691 AATT ^AATT
XmnI 1686 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 33 TCCGGA T^CCGGA
1120 TCCGGA
AlwNI 571 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
2563 CAGATGCTG
AocI 687 CCTGAGG CC^T[ACGT]AGG
2905 CCTGAGG
Aor51HI 294 AGCGCT AGC^GCT
767 AGCGCT
AspI 1054 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3001 GACACGGTC
BseAI 33 TCCGGA T^CCGGA
1120 TCCGGA
BspEI 33 TCCGGA T^CCGGA
1120 TCCGGA
BssSI 370 CTCGTG CACGAG(-5/-1)
1341 CTCGTG
BstAPI 738 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2271 GCAGAGGGTGC
BstXI 1539 CCACCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2814 CCAGCGGAATGG
Bsu36I 687 CCTGAGG CC^T[ACGT]AGG
2905 CCTGAGG
Eco47III 294 AGCGCT AGC^GCT
767 AGCGCT
Eco81I 687 CCTGAGG CC^T[ACGT]AGG
2905 CCTGAGG
KspI 503 CCGCGG CCGC^GG
2980 CCGCGG
MroI 33 TCCGGA T^CCGGA
1120 TCCGGA
NaeI 398 GCCGGC GCC^GGC
2176 GCCGGC
NgoMIV 398 GCCGGC G^CCGGC
2176 GCCGGC
PflFI 1054 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3001 GACACGGTC
PflMI 1033 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1632 CCAACACTTGG
PvuII 1127 CAGCTG CAG^CTG
2116 CAGCTG
SacI 1756 GAGCTC GAGCT^C
2248 GAGCTC
SacII 503 CCGCGG CCGC^GG
2980 CCGCGG
ScaI 2477 AGTACT AGT^ACT
2621 AGTACT
TfiI 2030 GATTC G^A[AT]TC
3078 GATTC
Tth111I 1054 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3001 GACACGGTC
Van91I 1033 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1632 CCAACACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
AviII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I XcmI XhoII
XmaCI XmaI